Open Access

Antitumor activity of Notch‑1 inhibition in human colorectal carcinoma cells

  • Authors:
    • Wangdi Liao
    • Guohua Li
    • Yu You
    • Hongping Wan
    • Qiong Wu
    • Chongwen Wang
    • Nonghua Lv
  • View Affiliations

  • Published online on: December 29, 2017     https://doi.org/10.3892/or.2017.6176
  • Pages: 1063-1071
  • Copyright: © Liao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

This study investigated the roles of Notch‑1 in colorectal carcinoma, to assess the mechanisms. The expression of Notch‑1 and its ligand-Jagged1 was detected in human colorectal carcinoma, colorectal adenoma, paracancerous tissue and normal colorectal tissue by immunohistochemistry. Colorectal carcinoma cell lines were utilized to confirm the expression of Notch‑1 in colorectal carcinoma cells. Lentiviral- encoding Notch‑1‑siRNA, as well as Notch‑1 inhibitor was employed to silence Notch‑1 expression and to inhibit Notch‑1 activity in HT29 cells, respectively. As evidenced, Notch‑1 and Jagged1 were highly expressed in colorectal carcinoma and colorectal adenoma tissues, compared with those in paracancerous tissue and normal colorectal tissue. However, the expression of Notch‑1 and Jagged1 was comparable in colorectal carcinoma and colorectal adenoma tissues, and in paracancerous and normal colorectal tissues. After screening colorectal carcinoma cell lines, Notch‑1 was extensively expressed in COLO 205, HT29, SW480 and SW1116 cells, but slightly expressed in LoVo cells. Subsequently, HT29 cell line was selected to investigate the roles of Notch‑1 in tumor cell growth and apoptosis. Lenti-viral encoding Notch‑1 siRNA significantly decreased Notch‑1 expression, inhibited cell growth, arrested the cell cycle at G1 phase and promoted apoptosis. These effects were further confirmed by the Notch‑1 inhibitor DAPT. Additionally, we evidenced that Notch‑1 silence promoted P21 and PUMA expression in HT29 cells. Taken together, Notch‑1 is an oncogene in colorectal carcinoma and the inhibition of Notch‑1 could delay the cell growth and promote apoptosis in colorectal cancer.
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March-2018
Volume 39 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Liao W, Li G, You Y, Wan H, Wu Q, Wang C and Lv N: Antitumor activity of Notch‑1 inhibition in human colorectal carcinoma cells. Oncol Rep 39: 1063-1071, 2018.
APA
Liao, W., Li, G., You, Y., Wan, H., Wu, Q., Wang, C., & Lv, N. (2018). Antitumor activity of Notch‑1 inhibition in human colorectal carcinoma cells. Oncology Reports, 39, 1063-1071. https://doi.org/10.3892/or.2017.6176
MLA
Liao, W., Li, G., You, Y., Wan, H., Wu, Q., Wang, C., Lv, N."Antitumor activity of Notch‑1 inhibition in human colorectal carcinoma cells". Oncology Reports 39.3 (2018): 1063-1071.
Chicago
Liao, W., Li, G., You, Y., Wan, H., Wu, Q., Wang, C., Lv, N."Antitumor activity of Notch‑1 inhibition in human colorectal carcinoma cells". Oncology Reports 39, no. 3 (2018): 1063-1071. https://doi.org/10.3892/or.2017.6176