Construction and investigation of lncRNA-associated ceRNA regulatory network in papillary thyroid cancer

  • Authors:
    • Yangjing Zhao
    • Hui Wang
    • Chengjiang Wu
    • Meina Yan
    • Haojie Wu
    • Jingzhe Wang
    • Xinxin Yang
    • Qixiang Shao
  • View Affiliations

  • Published online on: January 10, 2018     https://doi.org/10.3892/or.2018.6207
  • Pages: 1197-1206
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Abstract

Increasing evidence has experimentally proved the competitive endogenous RNA (ceRNA) hypothesis that long non-coding RNA (lncRNA) can affect the expression of RNA targets by competitively combining microRNA (miRNA) via miRNA response elements. However, an extensive ceRNA network of thyroid carcinoma in a large cohort has not been evaluated. We analyzed the RNAseq and miRNAseq data of 348 cases of primary papillary thyroid cancer (PTC) patients with clinical information downloaded from The Cancer Genome Atlas (TCGA) project to search for potential biomarkers or therapeutic targets. A computational approach was applied to build an lncRNA-miRNA-mRNA regulatory network of PTC. In total, 780 lncRNAs were detected as collectively dysregulated lncRNAs in all 3 PTC variants compared with normal tissues (fold change >2 and false discovery rate <0.05). The interactions among 45 lncRNAs, 13 miRNAs and 86 mRNAs constituted a ceRNA network of PTC. Nine out of the 45 aberrantly expressed lncRNAs were related to the clinical features of PTC patients. However, the expression levels of 3 lncRNAs (LINC00284, RBMS3-AS1 and ZFX-AS1) were identified to be tightly correlated with the patients overall survival (log-rank, P<0.05). The present study identified a list of specific lncRNAs associated with PTC progression and prognosis. This complex ceRNA interaction network in PTC may provide guidance for better understanding the molecular mechanisms underlying PTC.
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March-2018
Volume 39 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhao Y, Wang H, Wu C, Yan M, Wu H, Wang J, Yang X and Shao Q: Construction and investigation of lncRNA-associated ceRNA regulatory network in papillary thyroid cancer. Oncol Rep 39: 1197-1206, 2018.
APA
Zhao, Y., Wang, H., Wu, C., Yan, M., Wu, H., Wang, J. ... Shao, Q. (2018). Construction and investigation of lncRNA-associated ceRNA regulatory network in papillary thyroid cancer. Oncology Reports, 39, 1197-1206. https://doi.org/10.3892/or.2018.6207
MLA
Zhao, Y., Wang, H., Wu, C., Yan, M., Wu, H., Wang, J., Yang, X., Shao, Q."Construction and investigation of lncRNA-associated ceRNA regulatory network in papillary thyroid cancer". Oncology Reports 39.3 (2018): 1197-1206.
Chicago
Zhao, Y., Wang, H., Wu, C., Yan, M., Wu, H., Wang, J., Yang, X., Shao, Q."Construction and investigation of lncRNA-associated ceRNA regulatory network in papillary thyroid cancer". Oncology Reports 39, no. 3 (2018): 1197-1206. https://doi.org/10.3892/or.2018.6207