β‑arrestin2 promotes 5‑FU‑induced apoptosis via the NF‑κB pathway in colorectal cancer

  • Authors:
    • Weixia Ren
    • Tingting Wang
    • Xiangjun He
    • Qi Zhang
    • Jianhua Zhou
    • Fangfang Liu
    • Fangfang Gao
    • Yujun Zhang
    • Yulan Liu
  • View Affiliations

  • Published online on: March 30, 2018     https://doi.org/10.3892/or.2018.6340
  • Pages: 2711-2720
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Abstract

It has been demonstrated that β‑arrestin2 is involved in the initiation and development of many types of cancers. However, its role in colorectal cancer (CRC) remains poorly understood. The present study investigated the role of β‑arrestin2 in CRC using CRC patient tissues as well as the LoVo and HCT116 CRC cell lines. Briefly, significantly higher expression of β‑arrestin2 was observed in CRC tissues compared with normal colon tissues. In addition, the downregulation of β‑arrestin2 reduced 5‑FU‑induced apoptosis in the LoVo cells, while the overexpression of β‑arrestin2 increased the apoptosis of HCT116 cells in vitro. Furthermore, the downregulation of β‑arrestin2 reduced the expression of the pro‑apoptotic proteins cleaved‑caspase‑3 and Bax, and increased the expression of the anti‑apoptotic protein Bcl‑2 after 5‑FU treatment. In addition, the expression of p‑p65 was increased after the β‑arrestin2 downregulation and was decreased after the β‑arrestin2 overexpression. However, β‑arrestin2 downregulation had no effect on the proliferation, migration and invasion capacity of the LoVo cells. In conclusion, these results indicated that β‑arrestin2 promoted 5‑FU‑induced CRC cell apoptosis via the NF‑κB pathway and may be used as a prognosis marker for CRC.
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June-2018
Volume 39 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ren W, Wang T, He X, Zhang Q, Zhou J, Liu F, Gao F, Zhang Y and Liu Y: β‑arrestin2 promotes 5‑FU‑induced apoptosis via the NF‑κB pathway in colorectal cancer. Oncol Rep 39: 2711-2720, 2018.
APA
Ren, W., Wang, T., He, X., Zhang, Q., Zhou, J., Liu, F. ... Liu, Y. (2018). β‑arrestin2 promotes 5‑FU‑induced apoptosis via the NF‑κB pathway in colorectal cancer. Oncology Reports, 39, 2711-2720. https://doi.org/10.3892/or.2018.6340
MLA
Ren, W., Wang, T., He, X., Zhang, Q., Zhou, J., Liu, F., Gao, F., Zhang, Y., Liu, Y."β‑arrestin2 promotes 5‑FU‑induced apoptosis via the NF‑κB pathway in colorectal cancer". Oncology Reports 39.6 (2018): 2711-2720.
Chicago
Ren, W., Wang, T., He, X., Zhang, Q., Zhou, J., Liu, F., Gao, F., Zhang, Y., Liu, Y."β‑arrestin2 promotes 5‑FU‑induced apoptosis via the NF‑κB pathway in colorectal cancer". Oncology Reports 39, no. 6 (2018): 2711-2720. https://doi.org/10.3892/or.2018.6340