Girdin regulates the proliferation and apoptosis of pancreatic cancer cells via the PI3K/Akt signalling pathway

Wang,Sheng; Lei,Yiqun; Cai,Zeling; Ye,Xiaoman; Li,Lianhong; Luo,Xiagang; Yu,Chunzhao

doi:10.3892/or.2018.6469

Girdin regulates the proliferation and apoptosis of pancreatic cancer cells via the PI3K/Akt signalling pathway

Authors:
- Sheng Wang
- Yiqun Lei
- Zeling Cai
- Xiaoman Ye
- Lianhong Li
- Xiagang Luo
- Chunzhao Yu
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Affiliations: Department of General Surgery, The Second Clinical Medical School of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Gerontology, Jiangsu Province Hospital, Nanjing, Jiangsu 210029, P.R. China
Published online on: May 31, 2018 https://doi.org/10.3892/or.2018.6469
Pages: 599-608
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Girdin functions as an Akt phosphorylation enhancer (APE), which expedites the proliferation and survival of many types of tumours. However, the influence of Girdin on pancreatic cancer and the underlying molecular mechanisms have yet to be uncovered. Hence, in the present study, we sought to elucidate the function of Girdin in pancreatic cancer malignancy, particularly its role in pancreatic cancer cell proliferation, migration and apoptosis. Immunohistochemistry (IHC) was used to evaluate Girdin expression in pancreatic cancer tissues and to analyse its correlation with pathological grade. Girdin expression was further validated in pancreatic cancer cell lines (AsPC‑1, BxPC‑3 and PANC‑1), and human pancreatic ductal epithelial (HPNE) cells were used as a control. Recombinant adenovirus vectors containing Girdin‑siRNA were constructed to inhibit Girdin expression and were used in subsequent experiments to determine the effects of Girdin silencing on pancreatic cancer cells. Girdin silencing suppressed pancreatic cancer cell proliferation and induced pancreatic cancer cell apoptosis in vitro and in vivo. According to the results of further mechanistic investigations, Girdin may regulate cell processes through the phosphatidylinositol‑3‑kinase/protein kinase B (PI3K/Akt) signalling pathway to exert additive effects on pancreatic cancer.

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