MELK inhibition targets cancer stem cells through downregulation of SOX2 expression in head and neck cancer cells

  • Authors:
    • Lili Ren
    • Boya Deng
    • Vassiliki Saloura
    • Jae‑Hyun Park
    • Yusuke Nakamura
  • View Affiliations

  • Published online on: January 30, 2019     https://doi.org/10.3892/or.2019.6988
  • Pages: 2540-2548
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Abstract

Maternal embryonic leucine zipper kinase (MELK) has been reported to serve critical roles in the maintenance of stemness of cancer cells, although its mechanism remains unclear. Since SRY‑box 2 (SOX2) was demonstrated to be involved in self‑renewal and tumorigenicity of head and neck squamous cell carcinoma (HNSCC) and is aberrantly expressed in HNSCC tumors, the association between MELK and SOX2 was examined. Firstly, MELK inhibition was performed by small interfering RNA or MELK inhibitor OTS167, and it was determined that MELK inhibition by these approaches could decrease the SOX2 expression in HNSCC cells and OTS167 could suppress the SOX2 expression in a dose‑dependent manner. The present results indicated that MELK inhibition may target cancer stem cells (CSCs) through downregulation of the SOX2 gene. To further confirm the transcriptional regulation of SOX2, the transcription factors (TFs) were screened for SOX2 using a promoter‑binding TF assay followed by reverse transcription‑quantitative polymerase chain reaction and a decrease of the majority of the SOX2 TFs following MELK knockdown was observed. The present results provide evidence that MELK serves a key role in CSCs through the regulation of SOX2 and further indicates that MELK inhibition may also be promising for clinical applications in the treatment of HNSCC.
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April-2019
Volume 41 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ren L, Deng B, Saloura V, Park JH and Nakamura Y: MELK inhibition targets cancer stem cells through downregulation of SOX2 expression in head and neck cancer cells. Oncol Rep 41: 2540-2548, 2019.
APA
Ren, L., Deng, B., Saloura, V., Park, J., & Nakamura, Y. (2019). MELK inhibition targets cancer stem cells through downregulation of SOX2 expression in head and neck cancer cells. Oncology Reports, 41, 2540-2548. https://doi.org/10.3892/or.2019.6988
MLA
Ren, L., Deng, B., Saloura, V., Park, J., Nakamura, Y."MELK inhibition targets cancer stem cells through downregulation of SOX2 expression in head and neck cancer cells". Oncology Reports 41.4 (2019): 2540-2548.
Chicago
Ren, L., Deng, B., Saloura, V., Park, J., Nakamura, Y."MELK inhibition targets cancer stem cells through downregulation of SOX2 expression in head and neck cancer cells". Oncology Reports 41, no. 4 (2019): 2540-2548. https://doi.org/10.3892/or.2019.6988