Establishment and characterization of novel human oral squamous cell carcinoma cell lines from advanced‑stage tumors of buccal mucosa

  • Authors:
    • Nilesh P. Gawas
    • Sushant S. Navarange
    • Gopal L. Chovatiya
    • Pankaj Chaturvedi
    • Sanjeev K. Waghmare
  • View Affiliations

  • Published online on: February 7, 2019     https://doi.org/10.3892/or.2019.7003
  • Pages: 2289-2298
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Oral squamous cell carcinoma (OSCC) is a leading cause of mortality in India owing to the high percentage of tobacco chewers, smokers and alcohol consumption. OSCC is highly heterogeneous in nature; therefore poses a challenge in the treatment of the patient. To better understand the heterogeneity of the tumors, an in vitro cell line model is required. However, the efficiency of establishing cell lines from the oral tumors is low. In the present study, three novel cell lines, namely ACOSC3, ACOSC4, and ACOSC16, were isolated and characterized from advanced‑stage treatment‑naive OSCCs originating from the buccal mucosa. The three cell lines exhibited polygonal morphology, which is typical of epithelial cells. Furthermore, immunofluorescence revealed the expression of keratins 8 and 14, thereby confirming the epithelial origin of the cells. DNA content analysis of the three OSCC cell lines revealed aneuploidy. Furthermore, an in vitro orosphere assay revealed the formation of primary orospheres. Notably, the OSCC cell lines were able to give rise to tumors when administered subcutaneously into non‑obese diabetic/severe combined immune deficiency mice. The novelty of the cell lines was also validated by performing short tandem repeat profiling; the STR profiles of the present cell lines did not significantly match with any known established OSCC cell lines present in the DSMZ database, thereby confirming the unique identity of these lines. These cell lines established from tumor samples derived from Indian OSCC patients provide a valuable resource to understand the molecular mechanism involved in tumor resistance and recurrence.
View Figures
View References

Related Articles

Journal Cover

April-2019
Volume 41 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Gawas NP, Navarange SS, Chovatiya GL, Chaturvedi P and Waghmare SK: Establishment and characterization of novel human oral squamous cell carcinoma cell lines from advanced‑stage tumors of buccal mucosa. Oncol Rep 41: 2289-2298, 2019.
APA
Gawas, N.P., Navarange, S.S., Chovatiya, G.L., Chaturvedi, P., & Waghmare, S.K. (2019). Establishment and characterization of novel human oral squamous cell carcinoma cell lines from advanced‑stage tumors of buccal mucosa. Oncology Reports, 41, 2289-2298. https://doi.org/10.3892/or.2019.7003
MLA
Gawas, N. P., Navarange, S. S., Chovatiya, G. L., Chaturvedi, P., Waghmare, S. K."Establishment and characterization of novel human oral squamous cell carcinoma cell lines from advanced‑stage tumors of buccal mucosa". Oncology Reports 41.4 (2019): 2289-2298.
Chicago
Gawas, N. P., Navarange, S. S., Chovatiya, G. L., Chaturvedi, P., Waghmare, S. K."Establishment and characterization of novel human oral squamous cell carcinoma cell lines from advanced‑stage tumors of buccal mucosa". Oncology Reports 41, no. 4 (2019): 2289-2298. https://doi.org/10.3892/or.2019.7003