Predictive value of the ratio of 8‑hydroxydeoxyguanosine levels between cancerous and normal tissues in patients with stage II/III colorectal cancer

  • Authors:
    • Hiroshi Kitagawa
    • Yoshihiko Kitajima
    • Keita Kai
    • Sho Komukai
    • Tomokazu Tanaka
    • Yasuo Koga
    • Tatsuya Manabe
    • Hirokazu Noshirο
  • View Affiliations

  • Published online on: March 22, 2019     https://doi.org/10.3892/or.2019.7086
  • Pages: 3041-3050
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Abstract

Reactive oxygen species (ROS) accumulation is known to induce carcinogenesis and accelerate cancer progression. 8‑Hydroxydeoxyguanosine (8‑OHdG) is a specific marker of ROS‑mediated DNA damage. Therefore, we analysed 8‑OHdG levels in cancerous and normal tissue DNA via enzyme‑linked immunosorbent assay (ELISA) using 97 tissue specimens obtained from surgically‑treated patients with stage II/III colorectal cancer (CRC). Additionally, 8‑OHdG levels in these tissues were also assessed via quantitative immunohistochemistry (qIHC). To eliminate individual background variables, the ratio of 8‑OHdG levels between cancerous and normal tissues was calculated using both techniques. A comparative analysis demonstrated that the 8‑OHdG ratio in DNA was significantly correlated with both lymph node metastasis and lymphatic invasion. Multivariate analysis revealed that a high 8‑OHdG ratio in DNA was independently correlated with poor prognosis. These results suggest that the 8‑OHdG ratio in DNA reflects ROS‑induced cancer progression. Conversely, a low 8‑OHdG ratio as estimated via qIHC was an independent factor for poor prognosis. In Kaplan‑Meier analysis, the combination of a high 8‑OHdG ratio in DNA (ELISA) and a low 8‑OHdG ratio in cytoplasm (qIHC) was associated with markedly worse patient prognosis than other combinations. Combined evaluation of the 8‑OHdG ratio using ELISA and qIHC may be pivotal for predicting surgical outcomes for patients with stage II/III CRC.
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May-2019
Volume 41 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kitagawa H, Kitajima Y, Kai K, Komukai S, Tanaka T, Koga Y, Manabe T and Noshirο H: Predictive value of the ratio of 8‑hydroxydeoxyguanosine levels between cancerous and normal tissues in patients with stage II/III colorectal cancer. Oncol Rep 41: 3041-3050, 2019.
APA
Kitagawa, H., Kitajima, Y., Kai, K., Komukai, S., Tanaka, T., Koga, Y. ... Noshirο, H. (2019). Predictive value of the ratio of 8‑hydroxydeoxyguanosine levels between cancerous and normal tissues in patients with stage II/III colorectal cancer. Oncology Reports, 41, 3041-3050. https://doi.org/10.3892/or.2019.7086
MLA
Kitagawa, H., Kitajima, Y., Kai, K., Komukai, S., Tanaka, T., Koga, Y., Manabe, T., Noshirο, H."Predictive value of the ratio of 8‑hydroxydeoxyguanosine levels between cancerous and normal tissues in patients with stage II/III colorectal cancer". Oncology Reports 41.5 (2019): 3041-3050.
Chicago
Kitagawa, H., Kitajima, Y., Kai, K., Komukai, S., Tanaka, T., Koga, Y., Manabe, T., Noshirο, H."Predictive value of the ratio of 8‑hydroxydeoxyguanosine levels between cancerous and normal tissues in patients with stage II/III colorectal cancer". Oncology Reports 41, no. 5 (2019): 3041-3050. https://doi.org/10.3892/or.2019.7086