Open Access

NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma

  • Authors:
    • Qiuyun Guo
    • Na Han
    • Lei Shi
    • Li Yang
    • Xiaoxi Zhang
    • Yangmei Zhou
    • Shiying Yu
    • Mengxian Zhang
  • View Affiliations

  • Published online on: July 10, 2019     https://doi.org/10.3892/or.2019.7227
  • Pages: 963-972
  • Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glioblastoma (GBM) is the most common primary intracranial malignancy. GBM still exhibits high recurrence and mortality rates even following combined treatment with surgery, radiotherapy and chemotherapy, Therefore, the identification of novel therapeutic targets is urgent. Previous research has shown that nicotinamide phosphoribosyltransferase (NAMPT) plays a key role in cell metabolism and is closely related to the occurrence and development of many tumor types; yet, little is known concerning its relationship with GBM. Oncomine database analysis showed that the expression of NAMPT in GBM was higher than that in normal tissues; this finding was further confirmed by immunohistochemical staining of a tissue microarray. Data analysis with the R2 platform showed that patients with higher expression of NAMPT had worse prognoses than those with lower NAMPT expression. Using the GBM data in TCGA, four pathways enriched in the high NAMPT expression group were identified by gene set enrichment analysis (GSEA). NAMPT expression was knocked down in U87 and U251 GBM cells by lentiviral vectors carrying a small hairpin RNA (shRNA) targeting NAMPT. CCK‑8, colony formation, wound healing, Transwell and apoptosis assays were carried out. The results showed that NAMPT knockdown decreased cell proliferation, migration, and invasion and promoted apoptosis. U87 GBM cells were used in a model of subcutaneous tumorigenesis in nude mice. The results showed that NAMPT knockdown slowed the growth of tumors in vivo. Therefore, we speculate that NAMPT may be a potential prognostic and therapeutic biomarker for glioblastoma.
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September-2019
Volume 42 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Guo Q, Han N, Shi L, Yang L, Zhang X, Zhou Y, Yu S and Zhang M: NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma. Oncol Rep 42: 963-972, 2019.
APA
Guo, Q., Han, N., Shi, L., Yang, L., Zhang, X., Zhou, Y. ... Zhang, M. (2019). NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma. Oncology Reports, 42, 963-972. https://doi.org/10.3892/or.2019.7227
MLA
Guo, Q., Han, N., Shi, L., Yang, L., Zhang, X., Zhou, Y., Yu, S., Zhang, M."NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma". Oncology Reports 42.3 (2019): 963-972.
Chicago
Guo, Q., Han, N., Shi, L., Yang, L., Zhang, X., Zhou, Y., Yu, S., Zhang, M."NAMPT: A potential prognostic and therapeutic biomarker in patients with glioblastoma". Oncology Reports 42, no. 3 (2019): 963-972. https://doi.org/10.3892/or.2019.7227