Cryopreserved biopsy tissues of rectal cancer liver metastasis for assessment of anticancer drug response in vitro and in vivo

Zhang,Yuan; Huang,Wei‑Jian; Yang,Qiu‑Rui; Zhang,Hong‑Dan; Zhu,Xue‑Jing; Zeng,Min; Zhou,Xu; Wang,Zhen‑Yu; Li,Wei‑Jian; Jing,Hong‑Shu; Zhang,Xue‑Bin; Shi,Yao‑Ping; Hu,Hao; Yan,He‑Xin; Li,Zong‑Hai; Zhai,Bo

doi:10.3892/or.2019.7450

Cryopreserved biopsy tissues of rectal cancer liver metastasis for assessment of anticancer drug response in vitro and in vivo

Authors:
- Yuan Zhang
- Wei‑Jian Huang
- Qiu‑Rui Yang
- Hong‑Dan Zhang
- Xue‑Jing Zhu
- Min Zeng
- Xu Zhou
- Zhen‑Yu Wang
- Wei‑Jian Li
- Hong‑Shu Jing
- Xue‑Bin Zhang
- Yao‑Ping Shi
- Hao Hu
- He‑Xin Yan
- Zong‑Hai Li
- Bo Zhai
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Affiliations: Department of Tumor Interventional Oncology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200127, P.R. China, International Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, The Second Military Medical University, Shanghai 200438, P.R. China, Shanghai Celliver Biotechnology Co., Shanghai 201203, P.R. China, State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200032, P.R. China
Published online on: December 30, 2019 https://doi.org/10.3892/or.2019.7450
Pages: 405-414
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Living tumors are of great scientific value for clinical medicine and basic research, especially for drug testing. An increasing number of drug tests fail due to the use of imperfect models. The aim of the present study was to develop a novel method combining vitrification‑based cryopreservation of tumor biopsies and precision‑cut slice cultivation for the assessment of anticancer drug responses. Biological characteristics of rectal cancer liver metastasis biopsies could be retained by vitrification‑based cryopreservation. The patient‑derived xenograft models were successfully established using both fresh and warmed biopsy tissues. Precision‑cut slicing provided a similar three‑dimensional architecture and heterogeneity to the original tumor. The positive drug responses in the xenograft model were consistent with those in precision‑cut slice cultures in vitro. The present study demonstrated that live tumor biopsies could be preserved using vitrification‑based cryopreservation. The warmed tissues developed xenograft tumors, which were also useful for either in vivo or in vitro anticancer drug testing. Precision‑cut slices derived from the warmed tissues provided an efficient tool to assess anticancer drug response in vitro.

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