Open Access

Reduced expression of exosomal miR‑29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement

  • Authors:
    • Hideyuki Ohzawa
    • Akira Saito
    • Yuko Kumagai
    • Yuki Kimura
    • Hironori Yamaguchi
    • Yoshinori Hosoya
    • Alan Kawarai Lefor
    • Naohiro Sata
    • Joji Kitayama
  • View Affiliations

  • Published online on: February 17, 2020     https://doi.org/10.3892/or.2020.7505
  • Pages: 1081-1088
  • Copyright: © Ohzawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The peritoneal surface is the most frequent site of metastasis disease in patients with gastric cancer. Even after curative surgery and adjuvant chemotherapy, peritoneal recurrences often develop. Exosomes play pivotal roles in tumor metastasis via the transfer of microRNAs (miRNAs). In the present study, exosomes were isolated from peritoneal lavage fluid or ascites in 85 patients with gastric cancer and the relative expression levels of miR‑29s were examined. The expression of miR‑29a‑3p, miR‑29b‑3p and miR‑29c‑3p in peritoneal exosomes were all downregulated in patients with peritoneal metastases (PM) compared to those without PM. In 30 patients who underwent curative gastrectomy with serosa‑involved (T4) gastric cancer, 6 patients exhibited recurrence in the peritoneum within 12 months. The expression levels of miR‑29s at gastrectomy tended to be lower in these 6 patients than in the other 24 patients with significant differences in miR‑29b‑3p (P=0.003). When the patients were divided into two groups based on median levels of miR‑29s, peritoneal recurrence developed more frequently in patients with low expression of miR‑29b‑3p, and lower expression of miR‑29s were related with worse overall survival. miR‑29s are thought to play a suppressive role in the growth of disseminated peritoneal tumor cells. Reduced expression of miR‑29b in peritoneal exosomes is a strong risk factor of developing postoperative peritoneal recurrence.
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April-2020
Volume 43 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ohzawa H, Saito A, Kumagai Y, Kimura Y, Yamaguchi H, Hosoya Y, Lefor AK, Sata N and Kitayama J: Reduced expression of exosomal miR‑29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement. Oncol Rep 43: 1081-1088, 2020.
APA
Ohzawa, H., Saito, A., Kumagai, Y., Kimura, Y., Yamaguchi, H., Hosoya, Y. ... Kitayama, J. (2020). Reduced expression of exosomal miR‑29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement. Oncology Reports, 43, 1081-1088. https://doi.org/10.3892/or.2020.7505
MLA
Ohzawa, H., Saito, A., Kumagai, Y., Kimura, Y., Yamaguchi, H., Hosoya, Y., Lefor, A. K., Sata, N., Kitayama, J."Reduced expression of exosomal miR‑29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement". Oncology Reports 43.4 (2020): 1081-1088.
Chicago
Ohzawa, H., Saito, A., Kumagai, Y., Kimura, Y., Yamaguchi, H., Hosoya, Y., Lefor, A. K., Sata, N., Kitayama, J."Reduced expression of exosomal miR‑29s in peritoneal fluid is a useful predictor of peritoneal recurrence after curative resection of gastric cancer with serosal involvement". Oncology Reports 43, no. 4 (2020): 1081-1088. https://doi.org/10.3892/or.2020.7505