Mechanistic investigations of antitumor activity of a Rhodamine B‑oleanolic acid derivative bioconjugate Corrigendum in /10.3892/or.2021.7981

Macașoi,Ioana; Pavel,Ioana Zinuca; Moacă,Alina Elena; Avram,Ștefana; David,Vlad Laurențiu; Coricovac,Dorina; Mioc,Alexandra; Spandidos,Demetrios A.; Tsatsakis,Aristidis; Șoica,Codruța; Dumitrașcu,Victor; Dehelean,Cristina

doi:10.3892/or.2020.7666

Mechanistic investigations of antitumor activity of a Rhodamine B‑oleanolic acid derivative bioconjugate

Corrigendum in: /10.3892/or.2021.7981

Authors:
- Ioana Macașoi
- Ioana Zinuca Pavel
- Alina Elena Moacă
- Ștefana Avram
- Vlad Laurențiu David
- Dorina Coricovac
- Alexandra Mioc
- Demetrios A. Spandidos
- Aristidis Tsatsakis
- Codruța Șoica
- Victor Dumitrașcu
- Cristina Dehelean
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Affiliations: Department of Toxicology and Drug Industry, Faculty of Pharmacy, ‘Victor Babes’, University of Medicine and Pharmacy, 300041 Timisoara, Romania, Department of Pharmacognosy, Faculty of Pharmacy, ‘Victor Babes’, University of Medicine and Pharmacy, 300041 Timisoara, Romania, Department of Pediatric Surgery and Orthopedics, Faculty of Medicine, ‘Victor Babes’, University of Medicine and Pharmacy, 300041 Timisoara, Romania, Department of Anatomy, Physiology and Pathophysiology, Faculty of Pharmacy, ‘Victor Babes’, University of Medicine and Pharmacy, 300041 Timisoara, Romania, Laboratory of Clinical Virology, Medical School, University of Crete, 70013 Heraklion, Greece, Department of Forensic Sciences and Toxicology, Faculty of Medicine, University of Crete, 71003 Heraklion, Greece, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, ‘Victor Babes’, University of Medicine and Pharmacy, 300041 Timisoara, Romania, Department of Pharmacology, Faculty of Medicine, ‘Victor Babes’, University of Medicine and Pharmacy, 300041 Timisoara, Romania
Published online on: June 26, 2020 https://doi.org/10.3892/or.2020.7666
Pages: 1169-1183
Copyright: © Macașoi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Cancer remains a major health problem worldwide due to its high mortality rate. New therapeutic options highlight the importance of discovering new compounds that target the tumor microenvironment, interrupt angiogenesis and act selectively. The present study assessed the antitumor effect and investigated the mechanism of action of a rhodamine B‑conjugated oleanolic acid derivative (RhodOA). Consequently, the compound was tested on different human tumor cell lines (A375 melanoma, A549 lung adenocarcinoma and MDA‑MB‑231 breast adenocarcinoma) and on a non‑tumor cell line HaCaT human keratinocyte. RhodOA produced a dose‑dependent decrease in tumor cell viability especially in the melanoma cells while affecting the keratinocytes less. In melanoma cells, RhodOA reduced cell migration and produced condensation of cell nuclei and of actin fibers. Furthermore, an impairment in melanoma cell mitochondrial function was observed, while the mitochondrial function of keratinocytes was left intact. In the in ovo chorioallantoic membrane model, RhodOA elicited antiangiogenic effect, without showing irritation effect on the membrane. The study provides information on the selective antitumor effect of the derivative and its ability to inhibit cellular respiration, therefore RhodOA can be classified as ‘MITOCAN’.

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