Open Access

Circadian clock protein CRY1 prevents paclitaxel‑induced senescence of bladder cancer cells by promoting p53 degradation

  • Authors:
    • Min Jia
    • Bijia Su
    • Lijun Mo
    • Wen Qiu
    • Jiaxu Ying
    • Peng Lin
    • Bingxuan Yang
    • Danying Li
    • Dongxia Wang
    • Lili Xu
    • Hongwei Li
    • Zhongxin Zhou
    • Xing Li
    • Jinlong Li
  • View Affiliations

  • Published online on: December 30, 2020     https://doi.org/10.3892/or.2020.7914
  • Pages: 1033-1043
  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Bladder cancer is a common tumor type of the urinary system, which has high levels of morbidity and mortality. The first‑line treatment is cisplatin‑based combination chemotherapy, but a significant proportion of patients relapse due to the development of drug resistance. Therapy‑induced senescence can act as a ‘back‑up’ response to chemotherapy in cancer types that are resistant to apoptosis‑based anticancer therapies. The circadian clock serves an important role in drug resistance and cellular senescence. The aim of the present study was to investigate the regulatory effect of the circadian clock on paclitaxel (PTX)‑induced senescence in cisplatin‑resistant bladder cancer cells. Cisplatin‑resistant bladder cancer cells were established via long‑term cisplatin incubation. PTX induced apparent senescence in bladder cancer cells as demonstrated via SA‑β‑Gal staining, but this was not observed in the cisplatin‑resistant cells. The cisplatin‑resistant cells entered into a quiescent state with prolonged circadian rhythm under acute PTX stress. It was identified that the circadian protein cryptochrome1 (CRY1) accumulated in these quiescent cisplatin‑resistant cells, and that CRY1 knockdown restored PTX‑induced senescence. Mechanistically, CRY1 promoted p53 degradation via increasing the binding of p53 with its ubiquitin E3 ligase MDM2 proto‑oncogene. These data suggested that the accumulated CRY1 in cisplatin‑resistant cells could prevent PTX‑induced senescence by promoting p53 degradation.
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March-2021
Volume 45 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Jia M, Su B, Mo L, Qiu W, Ying J, Lin P, Yang B, Li D, Wang D, Xu L, Xu L, et al: Circadian clock protein CRY1 prevents paclitaxel‑induced senescence of bladder cancer cells by promoting p53 degradation. Oncol Rep 45: 1033-1043, 2021.
APA
Jia, M., Su, B., Mo, L., Qiu, W., Ying, J., Lin, P. ... Li, J. (2021). Circadian clock protein CRY1 prevents paclitaxel‑induced senescence of bladder cancer cells by promoting p53 degradation. Oncology Reports, 45, 1033-1043. https://doi.org/10.3892/or.2020.7914
MLA
Jia, M., Su, B., Mo, L., Qiu, W., Ying, J., Lin, P., Yang, B., Li, D., Wang, D., Xu, L., Li, H., Zhou, Z., Li, X., Li, J."Circadian clock protein CRY1 prevents paclitaxel‑induced senescence of bladder cancer cells by promoting p53 degradation". Oncology Reports 45.3 (2021): 1033-1043.
Chicago
Jia, M., Su, B., Mo, L., Qiu, W., Ying, J., Lin, P., Yang, B., Li, D., Wang, D., Xu, L., Li, H., Zhou, Z., Li, X., Li, J."Circadian clock protein CRY1 prevents paclitaxel‑induced senescence of bladder cancer cells by promoting p53 degradation". Oncology Reports 45, no. 3 (2021): 1033-1043. https://doi.org/10.3892/or.2020.7914