Open Access

Acanthopanax senticosus Harms extract causes G0/G1 cell cycle arrest and autophagy via inhibition of Rubicon in human liver cancer cells

  • Authors:
    • Yutaka Kawano
    • Maki Tanaka
    • Masaki Fujishima
    • Eri Okumura
    • Hideo Takekoshi
    • Kohichi Takada
    • Osamu Uehara
    • Yoshihiro Abiko
    • Hidekatsu Takeda
  • View Affiliations

  • Published online on: January 22, 2021     https://doi.org/10.3892/or.2021.7948
  • Pages: 1193-1201
  • Copyright: © Kawano et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Acanthopanax senticosus (Rupr. et Maxim) Harms (ASH), also known as Siberian ginseng or eleuthero, is a hardy shrub native to China, Korea, Russia and the northern region of Japan. ASH is used for the treatment of several diseases such as heart disease, hypertension, rheumatoid arthritis, allergies, chronic bronchitis, diabetes and cancer. In the present study, the inhibitory effect of the root extract of ASH (ASHE) on HuH‑7 and HepG2 liver cancer cells was examined. ASHE suppressed liver cancer cell proliferation by inducing cell cycle arrest at the G0/G1 phase, as well as apoptosis, as indicated by the increased number of Annexin V and 7‑AAD‑positive cells. Furthermore, the expression of LC3‑II, an autophagy marker, in these cells also increased post treatment with ASHE. LC3‑II induction was further enhanced by co‑treatment with chloroquine. Fluorescence and transmission electron micrographs of ASHE‑treated liver cancer cells showed the presence of an increased number of autophagic vesicles. A decreased protein expression level of run domain Beclin‑1‑interacting and cysteine‑rich domain‑containing, an autophagy inhibitor, with no change in RUBCN mRNA expression was observed, indicating activation of the autophagosome‑lysosome fusion step of autophagy. In conclusion, ASHE exerts cytostatic activity on liver cancer cells via both apoptosis and autophagy, and may serve as a potential therapeutic agent for management of liver cancer and autophagy‑related diseases.
View Figures
View References

Related Articles

Journal Cover

March-2021
Volume 45 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Kawano Y, Tanaka M, Fujishima M, Okumura E, Takekoshi H, Takada K, Uehara O, Abiko Y and Takeda H: Acanthopanax senticosus Harms extract causes G0/G1 cell cycle arrest and autophagy via inhibition of Rubicon in human liver cancer cells. Oncol Rep 45: 1193-1201, 2021.
APA
Kawano, Y., Tanaka, M., Fujishima, M., Okumura, E., Takekoshi, H., Takada, K. ... Takeda, H. (2021). Acanthopanax senticosus Harms extract causes G0/G1 cell cycle arrest and autophagy via inhibition of Rubicon in human liver cancer cells. Oncology Reports, 45, 1193-1201. https://doi.org/10.3892/or.2021.7948
MLA
Kawano, Y., Tanaka, M., Fujishima, M., Okumura, E., Takekoshi, H., Takada, K., Uehara, O., Abiko, Y., Takeda, H."Acanthopanax senticosus Harms extract causes G0/G1 cell cycle arrest and autophagy via inhibition of Rubicon in human liver cancer cells". Oncology Reports 45.3 (2021): 1193-1201.
Chicago
Kawano, Y., Tanaka, M., Fujishima, M., Okumura, E., Takekoshi, H., Takada, K., Uehara, O., Abiko, Y., Takeda, H."Acanthopanax senticosus Harms extract causes G0/G1 cell cycle arrest and autophagy via inhibition of Rubicon in human liver cancer cells". Oncology Reports 45, no. 3 (2021): 1193-1201. https://doi.org/10.3892/or.2021.7948