Open Access

Ribosome assembly factor PNO1 is associated with progression and promotes tumorigenesis in triple‑negative breast cancer

  • Authors:
    • Jie Li
    • Liya Liu
    • Youqin Chen
    • Meizhu Wu
    • Xiaoying Lin
    • Zhiqing Shen
    • Ying Cheng
    • Xiaoping Chen
    • Nathaniel Weygant
    • Xiangyan Wu
    • Lihui Wei
    • Thomas J. Sferra
    • Yuying Han
    • Xi Chen
    • Aling Shen
    • Jun Peng
  • View Affiliations

  • Published online on: April 21, 2022     https://doi.org/10.3892/or.2022.8319
  • Article Number: 108
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the expression of ribosome assembly factor partner of NOB1 homolog (PNO1) and its association with the progression of breast cancer (BC) in patients, as well as its biological function and underlying mechanism of action in BC cells. Bioinformatics and immunohistochemical analyses revealed that PNO1 expression was significantly increased in BC tissues and its high mRNA expression was associated with shorter overall survival (OS) and relapse‑free survival (RFS) of patients with BC, as well as multiple clinical characteristics (including advanced stage of NPI and SBR, etc.) of patients with BC. Biological functional studies revealed that transduction of lentivirus encoding sh‑PNO1 significantly downregulated PNO1 expression, reduced cell confluency and the number of BC cells in vitro and inhibited tumor growth in vivo. Moreover, PNO1 knockdown decreased the cell viability and arrested cell cycle progression at the G2/M phase, as well as downregulated cyclin B1 (CCNB1) and cyclin‑dependent kinase 1 (CDK1) protein expression in BC cells. Correlation analysis demonstrated that PNO1 expression was positively correlated with both CDK1 and CCNB1 expression in BC samples. Collectively, PNO1 was upregulated in BC and associated with BC patient survival, and PNO1 knockdown suppressed tumor growth in vitro and in vivo. In addition, positive regulation of CCNB1 and CDK1 may be one of the underlying mechanisms.
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June-2022
Volume 47 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li J, Liu L, Chen Y, Wu M, Lin X, Shen Z, Cheng Y, Chen X, Weygant N, Wu X, Wu X, et al: Ribosome assembly factor PNO1 is associated with progression and promotes tumorigenesis in triple‑negative breast cancer. Oncol Rep 47: 108, 2022.
APA
Li, J., Liu, L., Chen, Y., Wu, M., Lin, X., Shen, Z. ... Peng, J. (2022). Ribosome assembly factor PNO1 is associated with progression and promotes tumorigenesis in triple‑negative breast cancer. Oncology Reports, 47, 108. https://doi.org/10.3892/or.2022.8319
MLA
Li, J., Liu, L., Chen, Y., Wu, M., Lin, X., Shen, Z., Cheng, Y., Chen, X., Weygant, N., Wu, X., Wei, L., Sferra, T. J., Han, Y., Chen, X., Shen, A., Peng, J."Ribosome assembly factor PNO1 is associated with progression and promotes tumorigenesis in triple‑negative breast cancer". Oncology Reports 47.6 (2022): 108.
Chicago
Li, J., Liu, L., Chen, Y., Wu, M., Lin, X., Shen, Z., Cheng, Y., Chen, X., Weygant, N., Wu, X., Wei, L., Sferra, T. J., Han, Y., Chen, X., Shen, A., Peng, J."Ribosome assembly factor PNO1 is associated with progression and promotes tumorigenesis in triple‑negative breast cancer". Oncology Reports 47, no. 6 (2022): 108. https://doi.org/10.3892/or.2022.8319