Identification of ITGB4 as a novel tumor promoting gene in lung adenocarcinoma (LUAD)

Lu,Xuan; Lu,Xuan; Lu,Xuan; Lu,Xuan; Ma,Sai; Ma,Sai; Ma,Sai; Ma,Sai; Li,Ying; Li,Ying; Li,Ying; Li,Ying; Pan,Yunzhi; Pan,Yunzhi; Pan,Yunzhi; Pan,Yunzhi; Kang,Ningning; Kang,Ningning; Kang,Ningning; Kang,Ningning

doi:10.3892/or.2023.8689

Identification of ITGB4 as a novel tumor promoting gene in lung adenocarcinoma (LUAD)

Authors:
- Xuan Lu
- Sai Ma
- Ying Li
- Yunzhi Pan
- Ningning Kang
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Affiliations: School of Life Sciences, Hefei Normal University, Hefei, Anhui 230000, P.R. China, Department of Central Laboratory, The Affiliated Suzhou Hospital of Nanjing Medical University, Gusu School, Nanjing Medical University, Suzhou, Jiangsu 215000, P.R. China, Department of Pharmacy, The Affiliated Infectious Diseases Hospital of Soochow University, The Fifth People's Hospital of Suzhou, Suzhou, Jiangsu 215000, P.R. China, Department of Thoracic Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China
Published online on: December 20, 2023 https://doi.org/10.3892/or.2023.8689
Article Number: 30
Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

As the most frequently diagnosed cancer, lung cancer (LC) is the most common cause of cancer‑related death worldwide. In total, ~85% of malignant lung tumors belong to non‑small cell LC, of which ~50% are lung adenocarcinoma (LUAD). Integrin subunit β4 (ITGB4) is upregulated in lung glandular cancer and elevated ITGB4 levels predict an adverse clinical outcome. However, the biological function of ITGB4 in promoting LUAD progression remains unclear. In the present study, the upregulation of ITGB4 in LUAD tissue samples was demonstrated. To understand the biological role of ITGB4, ITGB4 expression was knocked down in A549 and PC9 cells through transfection with specific small interfering RNAs. The results demonstrated that the downregulation of ITGB4 attenuated A549 and PC9 cell proliferation, promoted cell apoptosis and inhibited colony formation, cell migration and cell invasion. To understand the mechanism of ITGB4, high throughput sequencing was performed using ITGB4‑knocked down A549 cells, followed by bioinformatics analysis. It was found that the genes upregulated by ITGB4 were significantly enriched in metabolism and related pathways, and the genes downregulated by ITGB4 were enriched in cell cycle and related pathways. In conclusion, the findings of the present study highlighted the oncogenic function of ITGB4 in LUAD and uncovered potential mechanisms fundamental to the progression of the disease.

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