Novel sequence tagged sites from the human 8p12-p21 chromosomal region involved in breast cancer
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- Published online on: March 1, 1997 https://doi.org/10.3892/or.4.2.367
- Pages: 367-369
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Abstract
Seventeen sequence-tagged sites (STSs) from the human 8p12-p21 chromosomal region were identified in a subset of nonchimeric yeast artificial chromosomes (YACs). YAC ends were cloned, sequenced and a PCR primer pair was designed for each of the STSs thus generated. STSs were mapped by means of PCR on somatic cell hybrids specific for chromosome 8. These STSs will contribute to the construction of physical, high-resolution genetic and transcriptional maps necessary to clone potential tumor suppressor genes of the 8p12-p21 region involved in breast cancer.