Rapid DNA quantification method in microplates using daunorubicine fluorescence quenching.
- Authors:
- Published online on: May 1, 1998 https://doi.org/10.3892/or.5.3.653
- Pages: 653-658
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Abstract
We have developed a rapid, sensitive and inexpensive method to quantify DNA in crude extracts or PCR reaction products using daunorubicine fluorescence quenching. We obtained a linear standard curve from 4.7 ng to 600 ng DNA and no interaction was observed in the presence of proteins.