A radioimmunoassay for the measurement of paclitaxel and taxanes in biological specimens using commercially available reagents.

  • Authors:
    • V Guillemard
    • N Landry
    • M Pagé
  • View Affiliations

  • Published online on: November 1, 1999     https://doi.org/10.3892/or.6.6.1257
  • Pages: 1257-1266
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Abstract

We have developed a rapid and sensitive radioimmunoassay for the measurement of paclitaxel and related taxanes in biological specimens using commercially available reagents. The presence of paclitaxel competitively inhibited the binding of radioactive paclitaxel to polyclonal anti-taxane antibody or anti-taxane monoclonal antibody. When using polyclonal antibody, this method detected paclitaxel in concentrations as low as 0.87 nM and was also effective in 20% methanol. This radioimmunoassay is useful for the measurement of paclitaxel and taxanes in biological specimens like culture medium of paclitaxel-producing microorganisms and may be useful for the measurement of paclitaxel and related compounds in other potential natural sources.

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Nov-Dec 1999
Volume 6 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Guillemard V, Landry N and Pagé M: A radioimmunoassay for the measurement of paclitaxel and taxanes in biological specimens using commercially available reagents.. Oncol Rep 6: 1257-1266, 1999.
APA
Guillemard, V., Landry, N., & Pagé, M. (1999). A radioimmunoassay for the measurement of paclitaxel and taxanes in biological specimens using commercially available reagents.. Oncology Reports, 6, 1257-1266. https://doi.org/10.3892/or.6.6.1257
MLA
Guillemard, V., Landry, N., Pagé, M."A radioimmunoassay for the measurement of paclitaxel and taxanes in biological specimens using commercially available reagents.". Oncology Reports 6.6 (1999): 1257-1266.
Chicago
Guillemard, V., Landry, N., Pagé, M."A radioimmunoassay for the measurement of paclitaxel and taxanes in biological specimens using commercially available reagents.". Oncology Reports 6, no. 6 (1999): 1257-1266. https://doi.org/10.3892/or.6.6.1257