Effect of wortmannin on the suppression of thermotolerance in tsAF8 cells
- Authors:
- Published online on: January 1, 2002 https://doi.org/10.3892/or.9.1.81
- Pages: 81-84
Metrics: Total
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Abstract
Phosphatidylinositol 3 kinases (PI-3 kinases) play a crucial role in the inositol phospholipid signaling pathway. The effect of wortmannin, an inhibitor of PI-3 kinases, on the suppression of thermotolerance development was investigated in the temperature-sensitive cell line tsAF8. When wortmannin was added immediately after the preheating, the thermotolerance was suppressed, with this suppression being dose-dependent from 0.01 to 0.1 μM, but not dose-dependent from 0.1 to 20 μM. Our results suggest that PI-3 kinases might play an important role in thermotolerance development in tsAF8 cells.