Piplartine induces caspase-mediated apoptosis in PC-3 human prostate cancer cells

  • Authors:
    • Eun-Hee Kong
    • Yun-Jin Kim
    • Young-Jin Kim
    • Hyo-Jin Cho
    • Sun-Nyoung Yu
    • Kwang-Youn Kim
    • Jeong-Hyun Chang
    • Soon-Cheol Ahn
  • View Affiliations

  • Published online on: October 1, 2008     https://doi.org/10.3892/or_00000075
  • Pages: 785-792
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Abstract

The present study examined the anti-proliferative effects of piplartine on the human prostate cancer cell line PC-3. This is the first report demonstrating the piplartine anti-cancer activity toward prostate cancer cell lines, although its precise mechanism of action is still not completely defined. In MTT assays, it preferentially inhibited growth of androgen-independent PC-3 cells in a dose-dependent (3-30 µM) and time-dependent (12-48 h) manner. In PC-3 cells, it showed an IC50 of 15 µM after 24 h of treatment. After a 24-30 µM treatment for 24 h, there were some reduction of cell volume, cell vacuolization, chromatin condensation and increased number of apoptotic cells visible by light and fluorescence microscopy. Agarose gel electrophoresis revealed that cells treated with piplartine exhibited DNA fragmentation. In addition, growth inhibition of PC-3 cells was associated with G2/M arrest and sub-G1 accumulation. Higher concentrations (24-30 µM) of piplartine modulated apoptosis-related protein expression by down-regulating cdc-2 expression and up-regulating PARP/procaspase-3 cleavage. Also, PC-3 cells treated with piplartine demonstrated caspase-3 activation, as observed with an in vitro caspase-3 colorimetric assay kit. Taken together, these results demonstrated that high concentrations of piplartine exhibited anti-proliferative and anti-cancer effects on PC-3 cells and that caspase-3-mediated PARP cleavage and cell cycle arrest at G2/M phase are involved in the underlying cellular mechanism of the apoptosis process.

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October 2008
Volume 20 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kong E, Kim Y, Kim Y, Cho H, Yu S, Kim K, Chang J and Ahn S: Piplartine induces caspase-mediated apoptosis in PC-3 human prostate cancer cells. Oncol Rep 20: 785-792, 2008.
APA
Kong, E., Kim, Y., Kim, Y., Cho, H., Yu, S., Kim, K. ... Ahn, S. (2008). Piplartine induces caspase-mediated apoptosis in PC-3 human prostate cancer cells. Oncology Reports, 20, 785-792. https://doi.org/10.3892/or_00000075
MLA
Kong, E., Kim, Y., Kim, Y., Cho, H., Yu, S., Kim, K., Chang, J., Ahn, S."Piplartine induces caspase-mediated apoptosis in PC-3 human prostate cancer cells". Oncology Reports 20.4 (2008): 785-792.
Chicago
Kong, E., Kim, Y., Kim, Y., Cho, H., Yu, S., Kim, K., Chang, J., Ahn, S."Piplartine induces caspase-mediated apoptosis in PC-3 human prostate cancer cells". Oncology Reports 20, no. 4 (2008): 785-792. https://doi.org/10.3892/or_00000075