ABCC10/MRP7 is associated with vinorelbine resistance in non-small cell lung cancer

  • Authors:
    • Yuji Bessho
    • Tetsuya Oguri
    • Hiroaki Ozasa
    • Takehiro Uemura
    • Hideo Sakamoto
    • Mikinori Miyazaki
    • Ken Maeno
    • Shigeki Sato
    • Ryuzo Ueda
  • View Affiliations

  • Published online on: January 1, 2009     https://doi.org/10.3892/or_00000217
  • Pages: 263-268
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Abstract

The non-small cell lung cancer (NSCLC) cells SK-LC6 and NCI-H23 were continuously exposed to vinorelbine (VNB), and the VNB-resistant clones, SK-LC6/VNB and H23/VNB were selected. Since SK-LS6/VNB and H23/VNB cells showed cross-resistance to certain anticancer drugs, such as paclitaxel and docetaxel, we examined the gene expression levels of drug efflux transporters of the ATP-binding cassette (ABC) family. We found that the gene expression of ABCB1/MDR1 and ABCC10/MRP7 in SK-LC6/VNB and H23/VNB cells was increased compared with that in SK-LS6 and NCI-H23 cells, whereas the expression of ABCC1/MRP1, ABCC2/MRP2, ABCC3/MRP3 and ABCG2/BCRP did not change among these cells. Treatment with ABCB1/MDR1 inhibitor verapamil and ABCC10/MRP7 inhibitor sulfin-pyrazone altered the sensitivity of SK-LC6/VNB cells to vinorelbine. To confirm the ABCC10/MRP7 activity, we transfected small interfering RNA against ABCC10/MRP7 to ABCC10/MRP7-expressing RERF-LC-AI cells resulting in the decrease of ABCC10/MRP7 expression concomitant with the alteration of VNB cytotoxicity. Moreover, we detected the expression of ABCC10/MRP7 in 12 of 17 NSCLC cells, whereas ABCB1/MDR1 was detected in only 3 of 17 NSCLC cells. These results indicate that ABCC10/MRP7 may confer VNB resistance in NSCLC.

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January 2009
Volume 21 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Bessho Y, Oguri T, Ozasa H, Uemura T, Sakamoto H, Miyazaki M, Maeno K, Sato S and Ueda R: ABCC10/MRP7 is associated with vinorelbine resistance in non-small cell lung cancer. Oncol Rep 21: 263-268, 2009.
APA
Bessho, Y., Oguri, T., Ozasa, H., Uemura, T., Sakamoto, H., Miyazaki, M. ... Ueda, R. (2009). ABCC10/MRP7 is associated with vinorelbine resistance in non-small cell lung cancer. Oncology Reports, 21, 263-268. https://doi.org/10.3892/or_00000217
MLA
Bessho, Y., Oguri, T., Ozasa, H., Uemura, T., Sakamoto, H., Miyazaki, M., Maeno, K., Sato, S., Ueda, R."ABCC10/MRP7 is associated with vinorelbine resistance in non-small cell lung cancer". Oncology Reports 21.1 (2009): 263-268.
Chicago
Bessho, Y., Oguri, T., Ozasa, H., Uemura, T., Sakamoto, H., Miyazaki, M., Maeno, K., Sato, S., Ueda, R."ABCC10/MRP7 is associated with vinorelbine resistance in non-small cell lung cancer". Oncology Reports 21, no. 1 (2009): 263-268. https://doi.org/10.3892/or_00000217