STAT3 blockade with shRNA enhances radiosensitivity in Hep-2 human laryngeal squamous carcinoma cells

  • Authors:
    • Xiaoming Li
    • Hairu Wang
    • Xiuying Lu
    • Bin Di
  • View Affiliations

  • Published online on: February 1, 2010     https://doi.org/10.3892/or_00000642
  • Pages: 345-353
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Abstract

Signal transducer and activator of transcription 3 (STAT3) is an oncogene aberrantly activated in many human tumors. We studied whether radiation combined with STAT3 siRNA enhances radiosensitivity of Hep-2 human laryngeal squamous carcinoma cells (Hep-2 cells). Firstly, STAT3 targeting recombinant plasmid was constructed. Hep-2 cells were transfected with expression vector of STAT3 siRNA using Lipofectamine 2000. Semiquantitive RT-PCR detected effective STAT3 mRNA down-regulation by STAT3 siRNA. Secondly, Hep-2 cells were radiated with different doses of γ-rays after transfection with STAT3 small interference RNA (siRNA). MTT assay showed cell proliferation decreased significantly (P<0.05) after STAT3 siRNA transfection combined with radiation. Thirdly, flow cytometry (FCM) demonstrated that cell apoptosis of combined treatment group increased significantly (P<0.05) and exhibited time dependency after 6 Gy irradiation (P<0.05). Simultaneously, STAT3, p-STAT3, Bcl-2, VEGF, p53 protein levels decreased in Hep-2 cells, with positive correlations between level of p-STAT3 and levels of Bcl-2, VEGF, p53, respectively (r=0.974, 0.988, 0.976, all P<0.01). Above all, specific siRNA targeting STAT3 gene is able to enhance the radiosensitivity in Hep-2 cells by regulating expression of Bcl-2, VEGF and p53 proteins.

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February 2010
Volume 23 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Li X, Wang H, Lu X and Di B: STAT3 blockade with shRNA enhances radiosensitivity in Hep-2 human laryngeal squamous carcinoma cells. Oncol Rep 23: 345-353, 2010.
APA
Li, X., Wang, H., Lu, X., & Di, B. (2010). STAT3 blockade with shRNA enhances radiosensitivity in Hep-2 human laryngeal squamous carcinoma cells. Oncology Reports, 23, 345-353. https://doi.org/10.3892/or_00000642
MLA
Li, X., Wang, H., Lu, X., Di, B."STAT3 blockade with shRNA enhances radiosensitivity in Hep-2 human laryngeal squamous carcinoma cells". Oncology Reports 23.2 (2010): 345-353.
Chicago
Li, X., Wang, H., Lu, X., Di, B."STAT3 blockade with shRNA enhances radiosensitivity in Hep-2 human laryngeal squamous carcinoma cells". Oncology Reports 23, no. 2 (2010): 345-353. https://doi.org/10.3892/or_00000642