The enhanced transcriptional activity of the V-val subtype of Epstein-Barr virus nuclear antigen 1 in epithelial cell lines

  • Authors:
    • Shi-Juan Mai
    • Dan Xie
    • Yu-Fan Huang
    • Feng-Wei Wang
    • Yi-Ji Liao
    • Hai-Xia Deng
    • Wen-Ju Liu
    • Wen-Feng Hua
    • Yi-Xin Zeng
  • View Affiliations

  • Published online on: May 1, 2010     https://doi.org/10.3892/or_00000779
  • Pages: 1417-1424
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Abstract

The Epstein-Barr virus encoded nuclear antigen 1 (EBNA1) is required for the replication and maintenance of the episomal EBV genome and for the transactivation of viral gene expression. EBNA1 has been classified into five subtypes, among which the V-val subtype was reported to be associated with nasopharyngeal carcinoma (NPC). Here we report a higher transcriptional activity of the V-val subtype of EBNA1 than for the prototype derived from B95.8 cells to transactivate FR-containing luciferase plasmid, which was mainly a consequence of the mutations in the carboxy-terminus of EBNA1. This interpretation was further supported by the finding that the variant form of EBNA1 has a higher binding affinity for the FR sequence than the prototype by electrophoretic mobility shift assays. The functional advantage of the V-val EBNA1 investigated in this study may contribute to the oncogenesis of NPC.

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May 2010
Volume 23 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Mai S, Xie D, Huang Y, Wang F, Liao Y, Deng H, Liu W, Hua W and Zeng Y: The enhanced transcriptional activity of the V-val subtype of Epstein-Barr virus nuclear antigen 1 in epithelial cell lines . Oncol Rep 23: 1417-1424, 2010.
APA
Mai, S., Xie, D., Huang, Y., Wang, F., Liao, Y., Deng, H. ... Zeng, Y. (2010). The enhanced transcriptional activity of the V-val subtype of Epstein-Barr virus nuclear antigen 1 in epithelial cell lines . Oncology Reports, 23, 1417-1424. https://doi.org/10.3892/or_00000779
MLA
Mai, S., Xie, D., Huang, Y., Wang, F., Liao, Y., Deng, H., Liu, W., Hua, W., Zeng, Y."The enhanced transcriptional activity of the V-val subtype of Epstein-Barr virus nuclear antigen 1 in epithelial cell lines ". Oncology Reports 23.5 (2010): 1417-1424.
Chicago
Mai, S., Xie, D., Huang, Y., Wang, F., Liao, Y., Deng, H., Liu, W., Hua, W., Zeng, Y."The enhanced transcriptional activity of the V-val subtype of Epstein-Barr virus nuclear antigen 1 in epithelial cell lines ". Oncology Reports 23, no. 5 (2010): 1417-1424. https://doi.org/10.3892/or_00000779