Isotope effect of <sup>13</sup>C‑enriched testosterone on human cells

Zhang,Mengqi; Wu,Xinchen; Yang,Wenning; Zhang,Tengfei

doi:10.3892/wasj.2022.144

Isotope effect of ¹³C‑enriched testosterone on human cells

Authors:
- Mengqi Zhang
- Xinchen Wu
- Wenning Yang
- Tengfei Zhang
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Affiliations: Department of Pediatrics, Harlem Hospital Center, Affiliated with Columbia University, New York, NY 10037, USA, Sanbo Brain Hospital, Capital Medical University, Beijing 100093, P.R. China, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, P.R. China
Published online on: February 16, 2022 https://doi.org/10.3892/wasj.2022.144
Article Number: 9
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study investigated the carbon isotope effect of carbon‑13 (¹³C)‑enriched testosterone on human cells; studies on the carbon isotope effects of a bioactive compound are limited. For this purpose, human osteoblasts, aortic endothelial cells and umbilical vein endothelial cells were treated with testosterone and ¹³C‑enriched testosterone. The cell growth rates and bioactivities of osteoblasts were measured. At physiological concentrations, testosterone promoted cell proliferation, whereas ¹³C‑enriched testosterone exerted a neutral and concentration‑independent effect on the cells. On the whole, the findings of the present study demonstrated that ¹³C‑enriched testosterone exerted a significant isotope effect on human cells; this needs to be taken into account in stable isotope‑based biology research.

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