Alterations in the expression of IGF‑I isoforms and binding proteins during the wound healing process

Garoufalia,Zoe; Papadopetraki,Argyro; Vardakostas,Dimitrios; Karatza,Elli; Philippou,Anastassios; Tsourouflis,Gerasimos; Kouraklis,Gregory; Mantas,Dimitrios

doi:10.3892/wasj.2022.148

Alterations in the expression of IGF‑I isoforms and binding proteins during the wound healing process

Authors:
- Zoe Garoufalia
- Argyro Papadopetraki
- Dimitrios Vardakostas
- Elli Karatza
- Anastassios Philippou
- Gerasimos Tsourouflis
- Gregory Kouraklis
- Dimitrios Mantas
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Affiliations: Second Department of Propaedeutic Surgery, ‘Laiko’ General Hospital, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece, Department of Physiology, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece
Published online on: March 3, 2022 https://doi.org/10.3892/wasj.2022.148
Article Number: 13
Copyright: © Garoufalia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

There is increasing evidence to indicate that insulin‑like growth factor (IGF)‑1 plays a crucial role in the regeneration of different tissues following injury. Notably, despite the escalating number of animal studies, studies investigating the role of IGF‑1 in the wound healing process in humans are fewer. In this context, the aim of the present study was to evaluate the variations in the expression IGF‑1 isoforms (IGF1‑Ea, IGF1‑Eb and IGF1‑Ec), as well as its binding protein and receptor (IGF‑BP3 and IGF‑1R) during wound healing in patients. The study population comprised of 21 patients presenting with the first episode of sacrococcygeal pilonidal disease. Samples were obtained during surgery, as well as on days 2, 7 and 14 post‑operatively. The expression levels of IGF‑1 isoforms, as well as that of its binding protein and receptor were evaluated using reverse transcription‑quantitative PCR. Statistical analyses were performed using GraphPad Prism software. The Kruskal‑Wallis test and Dunn's post hoc test were utilized. The results revealed a statistically significant difference in the expression of IGF‑BP3 and IGF‑1R during wound healing (P=0.014 and P=0.018, respectively). Specifically, the pairwise post‑hoc Dunn test indicated that IGF‑BP3 expression was significantly decreased on the 2nd post‑operative day compared to the day of surgery, while IGF‑1R expression was significantly increased at 14 days post‑operatively. The expression of the remaining IGF‑1 isoforms was not significantly altered during wound healing. On the whole, as demonstrated herein, IGF‑BP3 and IGF‑1R appear to play a crucial role during the wound healing process, particularly in patients with large open wounds following pilonidal disease treatment. Further studies are warranted to evaluate the exact role, as well as the possible use of these proteins as enhancers of wound healing.

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