Open Access

Inhibitory effect of Salvia plebeia leaf extract on ultraviolet‑induced photoaging‑associated ion channels and enzymes

  • Authors:
    • You‑Jin Chang
    • Dong‑Ung Lee
    • Da Yeong Nam
    • Sung Min Cho
    • Seungug Hong
    • Joo Hyun Nam
    • Woo Kyung Kim
  • View Affiliations

  • Published online on: January 5, 2017     https://doi.org/10.3892/etm.2017.4025
  • Pages: 567-575
  • Copyright: © Chang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In traditional Korean/Asian medicine, Salvia plebeia R.Br. (S. plebeia) leaves are used to treat inflammatory diseases, including dermatitis, cough, asthma and toothache. Recently, S. plebeia leaves have been applied in skin care, as they promote skin lightening and elasticity. Therefore, the present study investigated the anti‑aging effects of S. plebeia leaf methanolic extract and its fractions (dichloromethane, ethylacetate and n‑butanol). The results of a whole‑cell patch clamp analysis indicated that the methanolic extract mediated ultraviolet (UV)‑induced photoaging‑associated ion channels, transient receptor potential vanilloid 1 (TRPV1) and calcium release‑activated calcium channel protein 1 (ORAI1) channel activity in HEK293T cells overexpressing TRPV1 or ORAI1 and STIM1. Electrophysiological analysis revealed that the butanol fraction inhibited capsaicin‑induced TRPV1 (84±8% at ‑60 mV/86±1% at 100 mV at 100 µg/ml) and ORAI1 (87±2% at ‑120 mV at 100 µg/ml) currents. Furthermore, the dichloromethane and hexane fractions inhibited tyrosinase activity by 32.4±0.69 and 22.6±0.96% at 330 µg/ml, respectively. Furthermore, the ethylacetate and butanol fractions inhibited elastase activity by 65.2±1.30 and 31.7±1.23% at 330 µg/ml, respectively. Tyrosinase and elastase, which are UV‑induced photoaging‑associated enzymes, regulate skin pigmentation and wrinkle formation, respectively. The results of the present study indicated that S. plebeia leaves may be a novel treatment for UV‑induced photoaging.

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February-2017
Volume 13 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chang YJ, Lee DU, Nam D, Cho S, Hong S, Nam J and Kim W: Inhibitory effect of Salvia plebeia leaf extract on ultraviolet‑induced photoaging‑associated ion channels and enzymes. Exp Ther Med 13: 567-575, 2017
APA
Chang, Y., Lee, D., Nam, D., Cho, S., Hong, S., Nam, J., & Kim, W. (2017). Inhibitory effect of Salvia plebeia leaf extract on ultraviolet‑induced photoaging‑associated ion channels and enzymes. Experimental and Therapeutic Medicine, 13, 567-575. https://doi.org/10.3892/etm.2017.4025
MLA
Chang, Y., Lee, D., Nam, D., Cho, S., Hong, S., Nam, J., Kim, W."Inhibitory effect of Salvia plebeia leaf extract on ultraviolet‑induced photoaging‑associated ion channels and enzymes". Experimental and Therapeutic Medicine 13.2 (2017): 567-575.
Chicago
Chang, Y., Lee, D., Nam, D., Cho, S., Hong, S., Nam, J., Kim, W."Inhibitory effect of Salvia plebeia leaf extract on ultraviolet‑induced photoaging‑associated ion channels and enzymes". Experimental and Therapeutic Medicine 13, no. 2 (2017): 567-575. https://doi.org/10.3892/etm.2017.4025