Relevance of nuclear localization and functions of RNA helicase A

  • Authors:
    • Hidetoshi Fujita
    • Takayuki Ohshima
    • Takayuki Oishi
    • Satoko Aratani
    • Ryouji Fujii
    • Akiyoshi Fukamizu
    • Toshihiro Nakajima
  • View Affiliations

  • Published online on: April 1, 2005     https://doi.org/10.3892/ijmm.15.4.555
  • Pages: 555-560
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Abstract

RNA helicase A (RHA) regulates gene expression at transcriptional and post-transcriptional levels. It is also known that RHA shuttles between the nucleus and the cytoplasm. RHA possesses nuclear transport domain which functions as a nuclear localization signal (NLS) and nuclear export signal (NES), and predominantly localizes in the nucleus. Here, we identified regions that influence cellular localization of RHA. We showed that double-stranded RNA binding domain I and II (dsRBD I and II) positively localized to the cytoplasm. Mutational analysis revealed that RNA-binding ability was important to localization of dsRBD to the cytoplasm. RHA mutants lacking association with Pol II complexes were partially spilled from the nucleus. Furthermore, a minimal transactivation domain (MTAD), which is minimal region to interact with Pol II complexes, mainly localized to the nucleus in comparison with GFP. Our results indicated that the intracellular localization of RHA might be regulated by not only NLS and NES but also functional domains.

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April 2005
Volume 15 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Fujita H, Ohshima T, Oishi T, Aratani S, Fujii R, Fukamizu A and Nakajima T: Relevance of nuclear localization and functions of RNA helicase A. Int J Mol Med 15: 555-560, 2005.
APA
Fujita, H., Ohshima, T., Oishi, T., Aratani, S., Fujii, R., Fukamizu, A., & Nakajima, T. (2005). Relevance of nuclear localization and functions of RNA helicase A. International Journal of Molecular Medicine, 15, 555-560. https://doi.org/10.3892/ijmm.15.4.555
MLA
Fujita, H., Ohshima, T., Oishi, T., Aratani, S., Fujii, R., Fukamizu, A., Nakajima, T."Relevance of nuclear localization and functions of RNA helicase A". International Journal of Molecular Medicine 15.4 (2005): 555-560.
Chicago
Fujita, H., Ohshima, T., Oishi, T., Aratani, S., Fujii, R., Fukamizu, A., Nakajima, T."Relevance of nuclear localization and functions of RNA helicase A". International Journal of Molecular Medicine 15, no. 4 (2005): 555-560. https://doi.org/10.3892/ijmm.15.4.555