Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo

  • Authors:
    • Wei Luo
    • Junxia Wang
    • Dengfeng Xu
    • Huili Bai
    • Yangli Zhang
    • Yuhong Zhang
    • Xiaosong Li
  • View Affiliations

  • Published online on: January 17, 2018     https://doi.org/10.3892/ijmm.2018.3396
  • Pages: 2169-2176
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Abstract

In the present study, an artificial zinc-finger transcription factor eukaryotic expression vector specifically recognizing and binding to the hepatitis B virus (HBV) enhancer (Enh) was constructed, which inhibited the replication and expression of HBV DNA. The HBV EnhI‑specific pcDNA3.1‑artificial transcription factor (ATF) vector was successfully constructed, and then transformed or injected into HepG2.2.15 cells and HBV transgenic mice, respectively. The results demonstrated that the HBV EnhI (1,070‑1,234 bp)‑specific ATF significantly inhibited the replication and transcription of HBV DNA in vivo and in vitro. The HBV EnhI‑specific ATF may be a meritorious component of progressive combination therapies for eliminating HBV DNA in infected patients. A radical cure for chronic HBV infection may become feasible by using this bioengineering technology.
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April-2018
Volume 41 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Luo W, Wang J, Xu D, Bai H, Zhang Y, Zhang Y and Li X: Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo. Int J Mol Med 41: 2169-2176, 2018
APA
Luo, W., Wang, J., Xu, D., Bai, H., Zhang, Y., Zhang, Y., & Li, X. (2018). Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo. International Journal of Molecular Medicine, 41, 2169-2176. https://doi.org/10.3892/ijmm.2018.3396
MLA
Luo, W., Wang, J., Xu, D., Bai, H., Zhang, Y., Zhang, Y., Li, X."Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo". International Journal of Molecular Medicine 41.4 (2018): 2169-2176.
Chicago
Luo, W., Wang, J., Xu, D., Bai, H., Zhang, Y., Zhang, Y., Li, X."Engineered zinc-finger transcription factors inhibit the replication and transcription of HBV in vitro and in vivo". International Journal of Molecular Medicine 41, no. 4 (2018): 2169-2176. https://doi.org/10.3892/ijmm.2018.3396