Naringin protects H9C2 cardiomyocytes from chemical hypoxia‑induced injury by promoting the autophagic flux via the activation of the HIF‑1α/BNIP3 signaling pathway

  • Authors:
    • Shanghai Li
    • Jiamei Jiang
    • Junyu Fang
    • Xingyue Li
    • Chunyan Huang
    • Weijun Liang
    • Keng Wu
  • View Affiliations

  • Published online on: April 15, 2021     https://doi.org/10.3892/ijmm.2021.4935
  • Article Number: 102
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Abstract

Naringin, a natural bioflavonoid, has been shown to exert protective effects in multiple cardiovascular diseases; however, the protective effects of naringin against hypoxic/ischemia‑induced myocardial are not yet fully understood. Autophagy is a vital factor involved in the pathogenesis of myocardial injury. The aim of the present study was to investigate the protective effects of naringin on H9c2 cells against chemical hypoxia [cobalt chloride (CoCl2)]‑induced injury. The role of autophagy and the hypoxia‑inducible factor‑1α (HIF‑1α)/Bcl‑2/BCL2 interacting protein 3 (BNIP3) signaling pathway in the protective effects of naringin were also assessed. The results revealed that naringin pre‑treatment significantly attenuated the CoCl2‑induced cytotoxicity and apoptosis, and also decreased caspase‑3 activity, which had been increased by CoCl2. In addition, CoCl2 increased Beclin‑1 expression, enhanced the IL3B‑II/IL3B‑I ratio and increased p62 expression in the H9C2 cells. Treatment with 3‑methyladenine (3‑MA), a selective inhibitor of autophagy, also blocked CoCl2‑induced cytotoxicity and apoptosis. Notably, treatment with bafilomycin A1 (Baf A1), an inhibitor of the vacuolar H+ ATPase of lysosomes, resulted in an increase in the upregulation of the LC3B‑II/LC3B‑I ratio, but did not further increase the LC3B‑II/LC3B‑I ratio compared with CoCl2 treatment. These results suggested that CoCl2 inhibited the autophagic flux, which resulted in myocardial cell damage. Furthermore, naringin pre‑treatment exacerbated Beclin 1 expression and the increased IL3B‑II/IL3B‑I ratio, and reduced p62 expression in CoCl2‑treated H9C2 cells. 3‑MA and Baf A1 both reversed the protective effects of naringin against CoCl2‑induced injury, indicating that naringin attenuated CoCl2‑induced myocardial cell injury by the increasing autophagic flux. Moreover, naringin treatment resulted in upregulated expression levels of HIF‑1α and BNIP3 in the CoCl2‑treated H9C2 cells. The inhibition of the HIF‑1α/BNIP3 signaling pathway using 3‑(5'‑hydroxymethyl‑2'‑furyl)‑1‑benzylindazole (an inhibitor of HIF‑1α) prevented the effects of naringin on the autophagic flux and reversed its protective effects against CoCl2‑induced injury. Taken together, these results suggest that naringin protects the H9C2 cells against CoCl2‑induced injury by enhancing the autophagic flux via the activation of the HIF‑1α/BNIP3 signaling pathway.

Introduction

Cardiovascular disorders pose a major health burden and are associated with high morbidity and mortality rates worldwide (1). Hypoxia/ischemia is a major and crucial feature of various cardiac pathologies, including congenital heart disease, myocardial infarction and ischemic heart disease (2-4). Therefore, effective prevention and advances in myocardial protective therapy are necessary for the improvement of myocardial ischemic disease. Naringin is a non-toxic natural bioflavonoid found in citrus fruit peel with multiple pharmacological properties, including anticancer, antioxidant, anti-apoptotic, anti-inflammatory, anti-diabetic, neuroprotective and cardioprotective functions (5-8). Recently, the cardioprotective properties of naringin in various cardiovascular disease models have attracted increasing attention (9,10). Naringin has been shown to protect H9C2 myocardial cells against doxorubicin-induced cardiotoxicity and apoptosis (11). Naringin has also been shown to attenuate anoxia/reoxygenation-induced injury by inhibiting apoptosis and oxidative stress, which is dependent on the Nrf2 signaling pathway (12). However, the cardioprotective effects of naringin on ischemic injury and the underlying mechanisms are not yet fully understood.

Autophagy, a lysosome-mediated degradation pathway, is involved in major areas of cardiovascular disorders; however, it is still contested whether autophagy exerts a protective effect on cell survival or whether it results in cell death (13-15). Recent studies have found that the dysfunction of autophagy plays a vital role in the development and progression of myocardial damage (16,17). Autophagy is a vital degradation process required to maintain intracellular homeostasis under conditions of hypoxic/ischemic injury (18,19). It has been confirmed that hypoxia enhances the flux of autophagosome formation, and restoring autophagy function is thus a novel promising therapeutic option for managing ischemic injury (13,20). Hypoxia-inducible factor-1α (HIF-1α) is the main regulator of the cellular response to hypoxia (21). HIF-1α is closely associated with the regulation of myocardial survival by activating the downstream protein, BCL2 interacting protein 3 (BNIP3) (22). Notably, studies have demonstrated that BNIP3 is involve in regulating autophagy (23,24). Recently, studies have revealed that the hypoxic/ischemic condition promotes HIF-1α expression, and the HIF-1α/BNIP3 signaling pathway also plays essential roles in hypoxia-induced autophagy (23-25). In addition, studies have confirmed that naringin exerts beneficial effects by regulating autophagy (14,26). Hence, the present study aimed to further investigate whether naringin attenuates ischemic injury via modulating autophagy and the HIF-1α/BNIP3 signaling pathway.

Cobalt chloride (CoCl2), a type of chemical hypoxia mimetic agent (27), is widely used to mimic hypoxic/ischemic conditions, triggering cell damage and stabilizing intracellular HIF-1α expression, and is a key regulator in the adaptation to a low concentration of oxygen and cellular survival (28). H9C2 embryonic rat cardiac cells are derived from rat embryonic hearts and are widely used as in vitro models for exploring the molecular mechanisms or protective strategies underlying cardiovascular disorders (29). Therefore, CoCl2-treated H9C2 cells were used as a hypoxic model in in vitro in the present study. The present study explored the protective effects of naringin against CoCl2 injury and further investigated the underlying mechanisms, focusing on the autophagic flux and HIF-1α/BNIP3 signaling pathway in H9C2 cells. The findings of the present study may provide novel scientific evidence in favor of the use of naringin for the prevention of hypoxic/ischemic injury, and may highlight potentially novel targets for the management of hypoxic/ischemic injury.

Materials and methods

Cell culture and treatment

Rat H9C2 cardiomyocytes were obtained from the American Tissue Culture Collection (ATCC). Cells were maintained in DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) FBS (Gibco; Thermo Fisher Scientific, Inc.) and 100 U/ml penicillin-streptomycin (Beyotime Institute of Biotechnology) in an incubator (Thermo Fisher Scientific Inc.) with 5% CO2 at 37°C.

To mimic hypoxic conditions, H9C2 cells were treated with various concentrations of CoCl2 (200, 400, 600, 800 or 1,000 µmol/l; Sigma-Aldrich; Merck KGaA) for different periods of time (0, 1.5, 3, 6, 12 and 24 h). To investigate the protective effects of naringin (Sigma-Aldrich; Merck KGaA) on CoCl2 injury, H9C2 cells were pre-treated with naringin (5, 10, 20 and 40 µmol/l) for 1 h prior to treatment with CoCl2 (400 µmol/l) for 12 h. Cells incubated in normoxic medium (DMEM, 10% FBS, and 1% penicillin-streptomycin solution) in an incubator with 5% CO2 at 37°C served as the control group.

Treatment with 3-methyladenine (3-MA), bafilomycin A1 (Baf A1) or 3-(5′-hydroxymethyl-2′-furyl)-1-benzylindazole (YC-1)

3-MA and Baf A1 were obtained from Santa Cruz Biotechnology, Inc. YC-1 was purchased from Cayman Chemical Co. All reagents were dissolved in DMEM containing 1% DMSO. To inhibit autophagy, H9C2 cells were incubated with 3-MA (5 mM) for 1 h prior to treatment with CoCl2 (400 µmol/l) for 12 h in the presence or absence of naringin (20 µmol/l). To block autophagosome fusion with lysosomes, H9C2 cells were treated with Baf A1 (10 nmol/l) and CoCl2 (400 µmol/l) for 12 h in the presence or absence of naringin (20 µmol/l). To inhibit the HIF-1α/BNIP3 signaling pathway, H9C2 cells were treated with YC-1 (10 µM) for 1 h prior to treatment with naringin (20 µmol/l) for 2 h followed by co-treatment with CoCl2.

Cell Counting kit-8 (CCK-8) assay

Cell viability was assessed using a CCK-8 kit (Beijing Solarbio Science & Technology Co., Ltd.) according to the manufacturer's protocol. Briefly, H9C2 cells were added to 96-well plates (5×103 cells/well) and cultured. Following treatment as described above, the media from each group were replaced with serum-free DMEM (90 µl) plus CCK-8 regent (10 µl), and incubated for 3 h at 37°C. The absorbance was measured at 450 nm using a microplate reader (Thermo Fisher Scientific, Inc.). Cell viability as a percentage was calculated by comparing the value of the treated group to that of the control group.

Total lactate dehydrogenase (LDH) release assay

Cell injury was assessed based on the amount of total LDH released into the culture supernatant, measured using an LDH kit (Beyotime Institute of Biotechnology) according to the manufacturer's protocol. Following centrifugation at 1,000 × g, the supernatant was collected and plated in 96-well plates. The absorbance was measured using a microplate reader (Thermo Fisher Scientific, Inc.) at 450 nm.

Annexin V-FITC/PI double staining assay

The apoptosis of H9C2 cells was measured using an Annexin V-FITC/PI apoptosis detection kit (Nanjing Keygen Biotech Co., Ltd.) according to the manufacturer's protocol. H9C2 cells were seeded in 6-well plates (1×106 cells/well), and treated as described above. After washing with PBS twice, the cells were re-suspended in Annexin V-binding buffer (500 µl) and stained with FITC-labelled Annexin V (5 µl) and PI (10 µl) for 15 min in the dark at 37°C. The rate of apoptosis was quantified using a flow cytometer (BD LSRFortessa X-20; BD Biosciences).

Caspase-3/caspase-9 activities assay

The H9C2 cells were seeded in 6-well plates at a density of 1×106 per well. Following treatment as described above, the cells were sonicated, centrifuged at 12,000 × g for 10 min at 4°C, and the supernatant was retained. The concentration of proteins was determined using a bicinchoninic acid kit (Thermo Fisher Scientific, Inc.). The activities of caspase-3 and caspase-9 was determined using a Caspase-3 Activity Assay kit (Beyotime Institute of Biotechnology) and Caspase-9 Activity Assay kit (Beyotime Institute of Biotechnology) in accordance with the manufacturer's protocol. Caspase-3 and caspase-9 activities were determined by cleavage of the Ac-DEVD-pNA and Ac-LEHD-pNA substrates, respectively, and the absorbance was measured at 405 nm. The data are presented as fold increases over the pre-treatment levels.

Western blot analysis

The treated H9C2 cells were lysed using RIPA lysis buffer (Beyotime Institute of Biotechnology) containing 1 mM PMSF (Beyotime Institute of Biotechnology) and centrifuged at 12,000 × g for 10 min at 4°C. The concentration of proteins was determined using a BCA kit. Equivalent quantities of proteins (40 µg) were loaded on a 10% SDS-gel, resolved using SDS-PAGE and transferred to PVDF membranes (EMD Millipore). After blocking with 5% non-fat milk in TBS containing 0.01% Tween-20 (TBST) overnight at 4°C, the membranes were incubated with the following primary antibodies all at a dilution of 1:2,000: Rabbit monoclonal anti-Beclin1 (#3495), rabbit polyclonal LC3B-II/I (#3868), rabbit polyclonal anti-p62 (#39749), rabbit polyclonal anti-HIF-1α (#14179), rabbit polyclonal anti-BNIP3 (#44060) and rabbit polyclonal anti-tubulin (#2128) at 4°C overnight. All antibodies were purchased from Cell Signaling Technology, Inc. After washing twice with TBST, the membranes were incubated with HRP-conjugated goat anti-rabbit secondary antibody (1:5,000; #7074; Cell Signaling Technology, Inc.) at room temperature for 2 h. Finally, the bands were visualized using enhanced chemiluminescence reagent (Invitrogen; Thermo Fisher Scientific, Inc.). Densitometric analysis was performed using the Bio-Rad Microscopic Imaging system and Odyssey Image software (LICOR).

Statistical analysis

All data were analyzed using GraphPad Prism version 6 (GraphPad Software Inc.). Data are expressed as the means ± standard deviation. Differences between multiple groups were compared using a one-way ANOVA followed by a post-hoc Tukey's test. P<0.05 was considered to indicate a statistically significant difference.

Results

Naringin inhibits CoCl2-induced injury to H9C2 cells

To investigate the toxic effects of CoCl2 on H9C2 cells, the cells were incubated with various concentrations of CoCl2 (200, 400, 600, 800 and 1,000 µmol/l) for different periods of time (1.5, 3, 6, 12 and 24 h). The results of CCK-8 assay revealed that CoCl2 significantly reduced cell viability in a concentration- and time-dependent manner (Fig. 1A). CoCl2 (400 µmol/l) treatment for 12 h significantly decreased cell viability (P<0.01) to 40% of the control; thus, this concentration and treatment duration were used in subsequent experiments. Subsequently, the protective effects of naringin on CoCl2-induced toxicity to H9C2 cells were assessed. CCK-8 assay revealed that treatment with various concentrations of naringin (5, 10, 20 or 40 µmol/l) alone for 24 h did not exert any cytotoxic effects on the cells (Fig. 1B), and naringin (10, 20 and 40 µmol/l) pre-treatment reversed the CoCl2-induced decrease in cell viability (Fig. 1C). Pre-treatment with naringin at 20 µmol/l significantly reversed the CoCl2-induced decrease in cell viability (P<0.01), and thus this concentration was used in subsequent experiments to explore the protective effects of naringin against CoCl2 injury. These results indicated that naringin protected the H9C2 cells against CoCl2-induced injury.

Naringin reduces the CoCl2-induced apoptosis of H9C2 cells

Subsequently, the effect of naringin on the apoptosis of CoCl2-treated H9C2 cells was assessed. Flow cytometric analysis (Fig. 2A and B) revealed that treatment with CoCl2 resulted in a notable increase in the apoptotic rate (P<0.01), and this was prevented by naringin pre-treatment (P<0.01). Caspase-3 and caspase-9 are essential for apoptosis during myocardial damage (30). As shown in Fig. 2, the activities of caspase-3 and caspase-9 (Fig. 2C) were increased by CoCl2 (P<0.01), and these effects were significantly reversed by naringin (P<0.05). Naringin treatment alone had no effect on apoptosis and caspase-3/caspase-9 activity. These results demonstrate that naringin mitigates the CoCl2-induced apoptosis of H9C2 cells.

CoCl2 induces the blockage of the autophagic flux and leads to myocardial cell injury

Autophagy is closely associated with cell death in the hypoxic-ischemic process (31,32). To confirm the role of autophagy in CoCl2-induced injury to H9C2 cells, the expression of autophagy-related markers was determined. Western blot analysis revealed that following treatment with CoCl2, the levels of Beclin 1 expression (P<0.01) and the LC3B-II/LC3B-I ratio (P<0.01) were both significantly upregulated compared with the control group (Fig. 3A). p62 is a marker of the autophagic flux that is degraded in autophagosomes (33). In the present study, CoCl2 treatment increased the expression of p62 in H9C2 cells (Fig. 3A).

To determine whether the autophagic response following CoCl2 treatment resulted in damage to the H9C2 cells, the CoCl2-treated cells were co-treated with 3-MA, a selective inhibitor of autophagic initiation. The results of CCK-8 assay revealed that cell viability was significantly increased in the 3-MA and CoCl2 co-treatment groups compared with CoCl2 treatment alone (Fig. 3B). 3-MA also attenuated the CoCl2-induced increase in apoptosis (Fig. 3C and D). 3-MA treatment alone did not affect cell viability and apoptosis compared with the control group. The increases in the expression levels of pro-autophagic proteins may be due to either enhanced autophagosome formation or reduced autophagosome degradation. Furthermore, western blot analysis revealed that treatment with Baf A1 (an inhibitor of the vacuolar H+ ATPase of lysosomes) resulted in the upregulation of the LC3II/LC3I ratio compared with the control group (Fig. 3E), indicating a block on the basal lysosomal-dependent degradation of autophagosome cargo. Notably, the LC3II/LC3I ratio in the CoCl2 group was upregulated compared with the Baf A1 group, and there was no significant difference in the LC3II/LC3I ratio between CoCl2 treatment alone and co-treatment with Baf A1 and CoCl2, indicating that CoCl2 reduced the autophagic flux. Taken together, these data suggest that CoCl2-induced H9C2 cell injury results in the blockage of autophagic clearance rather than in autophagosome formation.

Naringin protects H9C2 cells against CoCl2-induced injury by restoring the impaired autophagic flux

To further investigate the role of the autophagic flux in the protective effects of naringin against CoCl2-induced H9C2 cell injury, the effects of naringin on the levels of autophagy-related markers were detected. As shown in Fig. 4, western blot analysis revealed that naringin treatment further increased Beclin 1 expression (Fig. 4A and B) and the LC3II/LC3I ratio (Fig. 4A and C) compared with CoCl2 treatment. Naringin reversed the CoCl2-induced upregulation of p62 expression in H9C2 cells (Fig. 4A and D). Compared with the control group, naringin treatment alone had no effect on the expression of these autophagy-related proteins. These results suggested an increase in the autophagic flux induced by naringin pre-treatment. In addition, the inhibitors of autophagy (3-MA and Baf A1) both attenuated the naringin-induced upregulation of cell viability (Fig. 4E) and the downregulation of LDH release (Fig. 4F) in CoCl2-treated H9C2 cells. Flow cytometric analysis also revealed that 3-MA and Baf A1 both mitigated the naringin-induced decrease in the apoptotic rate (Fig. 4G and H), as well as caspase-3 activity (Fig. 4I) and caspase-9 activity (Fig. 4J) in CoCl2-treated H9C2 cells. These results suggest that naringin enhances the autophagic flux, including autophagosome formation and autophagic clearance, and mitigates CoCl2 injury in H9C2 cells.

Naringin attenuates CoCl2-induced injury and promotes the autophagic flux by promoting the activation of the HIF-1α/BNIP3 signaling pathway in H9C2 cells

The HIF-1α/BNIP3 signaling pathway has been shown to play an essential role in hypoxia-induced autophagy (28,29). In the present study, the effects of naringin on the HIF-1α/BNIP3 signaling pathway in CoCl2-treated H9C2 cells was then assessed. The results of western blot analysis revealed that naringin pre-treatment further increased the expression of HIF-1α (Fig. 5A and B) and BNIP3 (Fig. 5A and C) in CoCl2-treated H9C2 cells, indicating that naringin promoted the activity of the HIF-1α/BNIP3 signaling pathway in the CoCl2-treated H9C2 cells. Furthermore, YC-1, an inhibitor of HIF-1α, blocked the naringin-induced increase in the expression of HIF-1α (Fig. 5D and E) and BNIP3 (Fig. 5D and F) in CoCl2-treated H9C2 cells. On these basis, YC-1 also attenuated the naringin-induced upregulation of Beclin 1 expression (Fig. 5G and H) and the ratio of IL3B-II to IL3B-I (Fig. 5G and I), as well as the downregulation of p62 expression (Fig. 5G and J) in CoCl2-treated H9C2 cells. This suggested that HIF-1α/BNIP3 mediated the naringin-induced increase in the autophagic flux. Moreover, treatment with YC-1 eliminated the naringin-induced increase in cell viability (Fig. 5K) and the decrease in LDH release (Fig. 5L). These results indicated that naringin protects H9C2 cells against CoCl2 injury by enhancing autophagic flux via activation of the HIF-1α/BNIP3 signaling pathway.

Discussion

Hypoxia/ischemia can induce sudden cardiomyocyte death, and this may result in further cardiovascular diseases (4,34). Naringin possesses anti-apoptotic, antioxidant and cardioprotective properties in the cardiovascular system (11,12). However, the effects of naringin on hypoxia/ischemia injury remain undetermined. The results of the present study demonstrated that naringin protected H9C2 cells against hypoxia-induced injury by promoting the autophagic flux via activation of the HIF-1α/BNIP3 signaling pathway.

CoCl2 (a chemical hypoxia-mimetic agent)-induced H9C2 cell injury has been shown to be a suitable in vitro model of hypoxia-associated damage in cardiomyocytes (35,36), and was thus used to explore the underlying mechanisms in the present study. The mechanisms underlying naringin-induced cardioprotection against hypoxia injury in the CoCl2-treated H9C2 cells were determined. The results revealed that CoCl2 significantly reduced H9C2 cell viability in a concentration- and time-dependent manner, consistent with the findings of previous studies (27,37). Recently, naringin, which is the major active constituent of tomentose pummelo peel, has been found to exert cardioprotective effects in animal and cell damage models (12,38). Naringin has been shown to attenuated the effects of several damaging stimuli, such as doxorubicin (11), hyperglycemia (39) and anoxia/reoxygenation induced myocardial injury (13) via the inhibition of apoptosis and oxidative stress in vitro and in vivo. Consistent with the findings of these studies, the results of the present study demonstrated that naringin attenuated the CoCl2-induced decrease in cell viability and the increase in apoptosis. These results indicate that naringin protects H9C2 cells against CoCl2-induced injury.

Autophagy is an evolutionarily controlled lysosome-mediated process that eliminates damaged proteins and intracellular organelle, involved in a variety of physiological processes (40,41). An increasing number of studies have demonstrated that autophagy dysfunction is increased in the heart following hypoxia/ischemia, and restoring impaired autophagic flux may prevent myocardial damage against hypoxia/ischemia (42-44). Recent studies have also indicated that CoCl2 increases the levels of markers associated with the autophagic-lysosomal pathway, including Beclin1 (an indicator of initiation of autophagy), the conversion of LC3B-I to LC3B-II (a surrogate index of autophagosome formation) and p62 (a commonly used indicator of autophagic flux inhibition) (13,45). Consistent with these studies, the results of the present study also indicated that CoCl2 significantly increased Beclin 1 expression and the LC3B-II/LC3B-I ratio, and decreased p62 expression in H9C2 cells. Thereafter, it is was shown that 3-MA attenuated CoCl2-induced cell damage and apoptosis in H9C2 cells, indicating that reducing autophagosome formation contributed to CoCl2-induced myocardial damage. In addition, previous studies have revealed that autophagy also plays important roles in the regulation of cell death, particularly in apoptosis-signaling pathways (46,47). Hence, it was hypothesized that the impaired autophagic flux leads to an increase in the apoptosis of CoCl2-treated H9C2 cells. Increases in the levels of pro-autophagic proteins may be due to either an enhanced autophagosome formation or reduced autophagosome degradation (48,49). To distinguish between these two possibilities, the H9C2 cells were treated with Baf A1 in the present study. The results revealed that Baf A1 treatment alone resulted in the upregulation of the LC3II/LC3I ratio, indicating a block on the basal lysosomal-dependent degradation of autophagosome cargo. Notably, the LC3B-II/LC3B-I ratio in the CoCl2 group was upregulated compared with the Baf A1 group, and there was no significant difference in LC3II/LC3I ratio between CoCl2 and Baf A1 co-treatment group with the CoCl2 group, indicating that CoCl2 inhibited the autophagic flux. Taken together, these data suggest that CoCl2-induced H9C2 cell injury is the result of blockage of autophagic flux, as evidenced by the enhancement of autophagosome formation and the reduction of lysosome-mediated autophagosome degradation.

Recently, naringin has been shown to regulate autophagy in several types of diseases (38,50,51). Naringin has been shown to promote the levels of autophagy markers, such as LC3B-II/I and Beclin-1, and autophagy is involved in the protective effect of naringin on oxidative stress-induced apoptosis in nucleus pulposus cells (14). Feng et al (50) demonstrated that naringin reduced cerebral apoptotic cell death underlying ischemia-reperfusion via the regulation of the activation of mitophagy. However, the effects of naringin on autophagy or the autophagic flux under hypoxia/ischemic conditions have not yet been studied, at least to the best of our knowledge. To the best of our knowledge, the present study was the first to demonstrate that naringin pre-treatment further increased Beclin 1 expression and the IL3B-II/IL3B-I ratio in CoCl2-treated H9C2 cells, and that naringin pretreatment decreased p62 expression in CoCl2-treated H9C2 cells, suggesting that naringin restores the autophagic flux under hypoxic conditions. In addition, Baf A1 attenuated the protective effects of naringin on cell injury and apoptosis induced by CoCl2 in H9C2 cells. These results suggest that naringin rescues the CoCl2-induced impaired autophagic flux by promoting the initiation of autophagy and degradation, and then protects H9C2 cells against CoCl2-induced injury. Combined with the role of autophagy in CoCl2 injury as discussed above, the ability of naringin to promote lysosome-mediated autophagosome degradation was more potent than the ability to promote autophagosome formation, thus exerting a cardioprotective effect against hypoxia injury.

HIF-1α is an important transcription factor in the response to hypoxia, and BNIP3, a transcriptional target of HIF-1α, is upregulated in response to hypoxia (21,52,53). Notably, previous studies have demonstrated that the HIF-1α/BNIP3 signaling pathway is closely associated with hypoxia-induced autophagy (25,54). Previous studies have also indicated that CoCl2 can disrupt HIF-1α degradation, and increase HIF-1α and BNIP3 expression, thus inducing apoptosis and autophagic cell death (25,55). Consistent with these studies, the expression levels of HIF-1α and BNIP3 in H9C2 cells were also significantly increased following CoCl2 treatment in the present study. Notably, in the present study, it was shown that naringin further increased the HIF-1α and BNIP3 expression levels, activating the HIF-1α/BNIP3 signaling pathway. The HIF-1α inhibitor, YC-1, induced the inhibition of the HIF-1α/BNIP3 signaling pathway and reversed the naringin-induced increase in Beclin 1 expression, in the IL3B-II/IL3B-1 ratio, and the decrease in p62 expression, suggesting that the enhancement of the autophagic flux induced by naringin was mediated by the HIF-1α/BNIP3 signaling pathway. In addition, YC-1 also blocked the protective effects of naringin against CoCl2-induced injury. These results suggest that naringin protects the H9C2 cells against CoCl2-induced injury by enhancing the autophagic flux through the activation of the HIF-1α/BNIP3 signaling pathway.

Limitations still exist in the present study. First, the present study did not detect the change in the autophagic flux with IL-3 fluorescence staining. In addition, drugs were simply used to intervene with the HIF-1 signaling pathway without using gene silencing. Second, as all our assays were performed using cells, in future experiments, the authors aim to introduce tests and to investigate the underlying mechanisms in in vitro animal models, such as rats. Other studies have confirmed that myocardial ischemia injury induces the inflammatory response (56,57). Naringin also has been shown to exhibit anti-inflammatory activity (58,59). Hence, it is meaningful to explore the protective mechanism of naringin on myocardial ischemia injury focusing on the inflammatory response in the future studies. In addition, naringin has been shown to exhibit anti-inflammatory activity. In addition, further identification and confirmation of the precise mechanisms underlying naringin and HIF-1α/BNIP3 signaling pathway are required.

In conclusion, the present study demonstrated that naringin attenuated CoCl2-induced injury in H9C2 cells by restoring the autophagic flux via the activation of the HIF-1α/BNIP3 signaling pathway. These findings reinforce the cardioprotective effects of naringin against hypoxic injury and indicate that the HIF-1α/BNIP3 signaling pathway may serve as a novel therapeutic target for the management of hypoxia/ischemia injury.

Availability of data and materials

The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.

Authors' contributions

SL and JJ designed the experiments and contributed to the drafting of the manuscript. JF, CH and XL collated and analyzed the data. WL and KW performed the experiments and wrote the manuscript. JJ and CH wrote and revised the manuscript. SL and KW confirm the authenticity of all the raw data. All authors read and approved the final manuscript.

Ethics approval and consent to participate

Not applicable.

Patient consent for publication

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Acknowledgments

Not applicable.

Funding

The present study was supported by the Guangdong Medical Science And Technology Research Fund Project (grant nos. A2017494 and A2016267) and the Project of Traditional Chinese Medicine Bureau of Guangdong Province (grant no. 20181149).

References

1 

GBD 2017 Causes of Death Collaborators: Global, regional, and national age-sex-specific mortality for 282 causes of death in 195 countries and territories, 1980-2017: A systematic analysis for the global burden of disease study 2017. Lancet. 392:1736–1788. 2018. View Article : Google Scholar

2 

Aouiss A, Anka Idrissi D, Kabine M and Zaid Y: Update of inflammatory proliferative retinopathy: Ischemia, hypoxia and angiogenesis. Curr Res Transl Med. 67:62–71. 2019. View Article : Google Scholar : PubMed/NCBI

3 

Cordova Martinez A, Pascual Fernandez J, Fernandez Lazaro D and Alvarez Mon M: Muscular and heart adaptations of execise in hypoxia. Is training in slow hypoxy healthy? Med Clin (Barc). 148:469–474. 2017.In English, Spanish.

4 

Rodgers JL, Iyer D, Rodgers LE, Vanthenapalli S and Panguluri SK: Impact of hyperoxia on cardiac pathophysiology. J Cell Physiol. 234:12595–12603. 2019. View Article : Google Scholar : PubMed/NCBI

5 

Den Hartogh DJ and Tsiani E: Antidiabetic properties of naringenin: A citrus fruit polyphenol. Biomolecules. 9:992019. View Article : Google Scholar :

6 

Kwatra M, Kumar V, Jangra A, Mishra M, Ahmed S, Ghosh P, Vohora D and Khanam R: Ameliorative effect of naringin against doxorubicin-induced acute cardiac toxicity in rats. Pharm Biol. 54:637–647. 2016. View Article : Google Scholar

7 

Ming H, Chuang Q, Jiashi W, Bin L, Guangbin W and Xianglu J: Naringin targets Zeb1 to suppress osteosarcoma cell proliferation and metastasis. Aging (Albany NY). 10:4141–4151. 2018. View Article : Google Scholar

8 

Salehi B, Fokou PVT, Sharifi-Rad M, Zucca P, Pezzani R, Martins N and Sharifi-Rad J: The therapeutic potential of naringenin: A review of clinical trials. Pharmaceuticals (Basel). 12:112019. View Article : Google Scholar

9 

Adebiyi OA, Adebiyi OO and Owira PM: Naringin reduces hyperglycemia-induced cardiac fibrosis by relieving oxidative stress. PLoS One. 11:e01498902016. View Article : Google Scholar : PubMed/NCBI

10 

Park JH, Ku HJ, Kim JK, Park JW and Lee JH: Amelioration of high fructose-induced cardiac hypertrophy by naringin. Sci Rep. 8:94642018. View Article : Google Scholar : PubMed/NCBI

11 

Jian CY, Ouyang HB, Xiang XH, Chen JL, Li YX, Zhou X, Wang JY, Yang Y, Zhong EY, Huang WH and Zhang HW: Naringin protects myocardial cells from doxorubicin-induced apoptosis partially by inhibiting the p38MAPK pathway. Mol Med Rep. 16:9457–9463. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Chen RC, Sun GB, Wang J, Zhang HJ and Sun XB: Naringin protects against anoxia/reoxygenation-induced apoptosis in H9c2 cells via the Nrf2 signaling pathway. Food Funct. 6:1331–1344. 2015. View Article : Google Scholar : PubMed/NCBI

13 

Cai CC, Zhu JH, Ye LX, Dai YY, Fang MC, Hu YY, Pan SL, Chen S, Li PJ, Fu XQ and Lin ZL: Glycine protects against hypoxic-ischemic brain injury by regulating mitochondria-mediated autophagy via the AMPK pathway. Oxid Med Cell Longev. 2019:42485292019. View Article : Google Scholar : PubMed/NCBI

14 

Zhang Z, Wang C, Lin J, Jin H, Wang K, Yan Y, Wang J, Wu C, Nisar M, Tian N, et al: Therapeutic potential of naringin for intervertebral disc degeneration: Involvement of autophagy against oxidative stress-induced apoptosis in nucleus pulposus cells. Am J Chin Med. Oct 4–2018.Epub ahead of print. View Article : Google Scholar

15 

Sousa Fialho MD, Abd Jamil AH, Stannard GA and Heather LC: Hypoxia-inducible factor 1 signalling, metabolism and its therapeutic potential in cardiovascular disease. Biochim Biophys Acta Mol Basis Dis. 1865:831–843. 2019. View Article : Google Scholar

16 

Bravo-San Pedro JM, Kroemer G and Galluzzi L: Autophagy and mitophagy in cardiovascular disease. Circ Res. 120:1812–1824. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Sciarretta S, Maejima Y, Zablocki D and Sadoshima J: The role of autophagy in the heart. Annu Rev Physiol. 80:1–26. 2018. View Article : Google Scholar

18 

Qiao J, Huang J, Zhou M, Cao G and Shen H: Inhibition of HIF-1α restrains fracture healing via regulation of autophagy in a rat model. Exp Ther Med. 17:1884–1890. 2019.PubMed/NCBI

19 

Zimmerman MA, Biggers CD and Li PA: Rapamycin treatment increases hippocampal cell viability in an mTOR-independent manner during exposure to hypoxia mimetic, cobalt chloride. BMC Neurosci. 19:822018. View Article : Google Scholar : PubMed/NCBI

20 

Gallo S, Gatti S, Sala V, Albano R, Costelli P, Casanova E, Comoglio PM and Crepaldi T: Agonist antibodies activating the Met receptor protect cardiomyoblasts from cobalt chloride-induced apoptosis and autophagy. Cell Death Dis. 5:e11852014. View Article : Google Scholar : PubMed/NCBI

21 

Lee JW, Bae SH, Jeong JW, Kim SH and Kim KW: Hypoxia-inducible factor (HIF-1)alpha: Its protein stability and biological functions. Exp Mol Med. 36:1–12. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Wang H, Zhang D, Jia S, Huang S, Xiao L, Ma L, Liu G, Gong K and Xu L: Effect of sustained hypoxia on autophagy of genioglossus muscle-derived stem cells. Med Sci Monit. 24:2218–2224. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Zhang Y, Liu D, Hu H, Zhang P, Xie R and Cui W: HIF-1α/BNIP3 signaling pathway-induced-autophagy plays protective role during myocardial ischemia-reperfusion injury. Biomed Pharmacother. 120:1094642019. View Article : Google Scholar

24 

Liu XW, Lu MK, Zhong HT, Wang LH and Fu YP: Panax notoginseng saponins attenuate myocardial ischemia-reperfusion injury through the HIF-1α/BNIP3 pathway of autophagy. J Cardiovasc Pharmacol. 73:92–99. 2019. View Article : Google Scholar

25 

Wu H, Huang S, Chen Z, Liu W, Zhou X and Zhang D: Hypoxia-induced autophagy contributes to the invasion of salivary adenoid cystic carcinoma through the HIF-1α/BNIP3 signaling pathway. Mol Med Rep. 12:6467–6474. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Wang K, Peng S, Xiong S, Niu A, Xia M, Xiong X, Zeng G and Huang Q: Naringin inhibits autophagy mediated by PI3K-Akt-mTOR pathway to ameliorate endothelial cell dysfunction induced by high glucose/high fat stress. Eur J Pharmacol. 874:1730032020. View Article : Google Scholar : PubMed/NCBI

27 

Manu TM, Anand T, Pandareesh MD, Kumar PB and Khanum F: Terminalia arjuna extract and arjunic acid mitigate cobalt chloride-induced hypoxia stress-mediated apoptosis in H9c2 cells. Naunyn Schmiedebergs Arch Pharmacol. 392:1107–1119. 2019. View Article : Google Scholar : PubMed/NCBI

28 

Warbrick I and Rabkin SW: Hypoxia-inducible factor 1-alpha (HIF-1α) as a factor mediating the relationship between obesity and heart failure with preserved ejection fraction. Obes Rev. 20:701–712. 2019. View Article : Google Scholar : PubMed/NCBI

29 

Hescheler J, Meyer R, Plant S, Krautwurst D, Rosenthal W and Schultz G: Morphological, biochemical, and electrophysiological characterization of a clonal cell (H9c2) line from rat heart. Circ Res. 69:1476–1486. 1991. View Article : Google Scholar : PubMed/NCBI

30 

Haider N, Narula N and Narula J: Apoptosis in heart failure represents programmed cell survival, not death, of cardiomyocytes and likelihood of reverse remodeling. J Card Fail. 8(6 Suppl): S512–S517. 2002. View Article : Google Scholar

31 

Semenza GL: Hypoxia-inducible factor 1: Regulator of mitochondrial metabolism and mediator of ischemic preconditioning. Biochim Biophys Acta. 1813:1263–1268. 2011. View Article : Google Scholar

32 

Yadav AK, Yadav PK, Chaudhary GR, Tiwari M, Gupta A, Sharma A, Pandey AN, Pandey AK and Chaube SK: Autophagy in hypoxic ovary. Cell Mol Life Sci. 76:3311–3322. 2019. View Article : Google Scholar : PubMed/NCBI

33 

Lamark T, Svenning S and Johansen T: Regulation of selective autophagy: The p62/SQSTM1 paradigm. Essays Biochem. 61:609–624. 2017. View Article : Google Scholar : PubMed/NCBI

34 

Mallet RT, Manukhina EB, Ruelas SS, Caffrey JL and Downey HF: Cardioprotection by intermittent hypoxia conditioning: Evidence, mechanisms, and therapeutic potential. Am J Physiol Heart Circ Physiol. 315:H216–H232. 2018. View Article : Google Scholar : PubMed/NCBI

35 

Cheng CI, Lee YH, Chen PH, Lin YC, Chou MH and Kao YH: Cobalt chloride induces RhoA/ROCK activation and remodeling effect in H9c2 cardiomyoblasts: Involvement of PI3K/Akt and MAPK pathways. Cell Signal. 36:25–33. 2017. View Article : Google Scholar : PubMed/NCBI

36 

Munoz-Sanchez J and Chanez-Cardenas ME: The use of cobalt chloride as a chemical hypoxia model. J Appl Toxicol. 39:556–570. 2019. View Article : Google Scholar

37 

Shi YN, Zhang XQ, Hu ZY, Zhang CJ, Liao DF, Huang HL and Qin L: Genistein protects H9c2 cardiomyocytes against chemical hypoxia-induced injury via inhibition of apoptosis. Pharmacology. 103:282–290. 2019. View Article : Google Scholar : PubMed/NCBI

38 

Caglayan C: The effects of naringin on different cyclophosphamide-induced organ toxicities in rats: Investigation of changes in some metabolic enzyme activities. Environ Sci Pollut Res Int. 26:26664–26673. 2019. View Article : Google Scholar : PubMed/NCBI

39 

You Q, Wu Z, Wu B, Liu C, Huang R, Yang L, Guo R, Wu K and Chen J: Naringin protects cardiomyocytes against hyperglycemia-induced injuries in vitro and in vivo. J Endocrinol. 230:197–214. 2016. View Article : Google Scholar : PubMed/NCBI

40 

Moosavi MA and Djavaheri-Mergny M: Autophagy: New insights into mechanisms of action and resistance of treatment in acute promyelocytic leukemia. Int J Mol Sci. 20:35592019. View Article : Google Scholar :

41 

Ravanan P, Srikumar IF and Talwar P: Autophagy: The spotlight for cellular stress responses. Life Sci. 188:53–67. 2017. View Article : Google Scholar : PubMed/NCBI

42 

Gao C, Wang R, Li B, Guo Y, Yin T, Xia Y, Zhang F, Lian K, Liu Y, Wang H, et al: TXNIP/Redd1 signaling and excessive autophagy: A novel mechanism of myocardial ischemia/reperfusion injury in mice. Cardiovasc Res. 116:645–657. 2019. View Article : Google Scholar

43 

McCormick J, Knight RA, Barry SP, Scarabelli TM, Abounit K, Latchman DS and Stephanou A: Autophagy in the stress-induced myocardium. Front Biosci (Elite Ed). 4:2131–2141. 2012. View Article : Google Scholar

44 

Ryter SW, Lee SJ, Smith A and Choi AM: Autophagy in vascular disease. Proc Am Thorac Soc. 7:40–47. 2010. View Article : Google Scholar : PubMed/NCBI

45 

Chen Y, Yan Q, Xu Y, Ye F, Sun X, Zhu H and Wang H: BNIP3-mediated autophagy induced inflammatory response and inhibited VEGF expression in cultured retinal pigment epithelium cells under hypoxia. Curr Mol Med. 19:395–404. 2019. View Article : Google Scholar : PubMed/NCBI

46 

Glick D, Barth S and Macleod KF: Autophagy: Cellular and molecular mechanisms. J Pathol. 221:3–12. 2010. View Article : Google Scholar : PubMed/NCBI

47 

Maiuri MC, Zalckvar E, Kimchi A and Kroemer G: Self-eating and self-killing: Crosstalk between autophagy and apoptosis. Nat Rev Mol Cell Biol. 8:741–752. 2007. View Article : Google Scholar : PubMed/NCBI

48 

Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, Kodama A, Ishihara T, Mizushima T and Mizushima N: An autophagic flux probe that releases an internal control. Mol Cell. 64:835–849. 2016. View Article : Google Scholar : PubMed/NCBI

49 

Wu J and Lipinski MM: Autophagy in neurotrauma: Good, bad, or dysregulated. Cells. 8:6932019. View Article : Google Scholar :

50 

Feng J, Chen X, Lu S, Li W, Yang D, Su W, Wang X and Shen J: Naringin attenuates cerebral ischemia-reperfusion injury through inhibiting peroxynitrite-mediated mitophagy activation. Mol Neurobiol. 55:9029–9042. 2018. View Article : Google Scholar : PubMed/NCBI

51 

Raha S, Yumnam S, Hong GE, Lee HJ, Saralamma VV, Park HS, Heo JD, Lee SJ, Kim EH, Kim JA and Kim GS: Naringin induces autophagy-mediated growth inhibition by downregulating the PI3K/Akt/mTOR cascade via activation of MAPK pathways in AGS cancer cells. Int J Oncol. 47:1061–1069. 2015. View Article : Google Scholar : PubMed/NCBI

52 

Adams JM, Difazio LT, Rolandelli RH, Luján JJ, Haskó G, Csóka B, Selmeczy Z and Németh ZH: HIF-1: A key mediator in hypoxia. Acta Physiol Hung. 96:19–28. 2009. View Article : Google Scholar : PubMed/NCBI

53 

Chinnadurai G, Vijayalingam S and Gibson SB: BNIP3 subfamily BH3-only proteins: Mitochondrial stress sensors in normal and pathological functions. Oncogene. 27(Suppl 1): S114–S127. 2008. View Article : Google Scholar

54 

Lu N, Li X, Tan R, An J, Cai Z, Hu X, Wang F, Wang H, Lu C and Lu H: HIF-1α/beclin1-mediated autophagy is involved in neuroprotection induced by hypoxic preconditioning. J Mol Neurosci. 66:238–250. 2018. View Article : Google Scholar : PubMed/NCBI

55 

Chen R, Jiang T, She Y, Xu J, Li C, Zhou S, Shen H, Shi H and Liu S: Effects of cobalt chloride, a hypoxia-mimetic agent, on autophagy and atrophy in skeletal C2C12 myotubes. Biomed Res Int. 2017:70975802017.PubMed/NCBI

56 

Qian X, Zhu M, Qian W and Song J: Vitamin D attenuates myocardial ischemia-reperfusion injury by inhibiting inflammation via suppressing the RhoA/ROCK/NF-κB pathway. Biotechnol Appl Biochem. 66:850–857. 2019. View Article : Google Scholar : PubMed/NCBI

57 

Vinten-Johansen J, Jiang R, Reeves JG, Mykytenko J, Deneve J and Jobe LJ: Inflammation, proinflammatory mediators and myocardial ischemia-reperfusion Injury. Hematol Oncol Clin North Am. 21:123–145. 2007. View Article : Google Scholar : PubMed/NCBI

58 

Zhao H, Liu M, Liu H, Suo R and Lu C: Naringin protects endothelial cells from apoptosis and inflammation by regulating the hippo-YAP pathway. Biosci Rep. 40:BSR201934312020. View Article : Google Scholar : PubMed/NCBI

59 

Sun LJ, Qiao W, Xiao YJ, Cui L, Wang X and Ren WD: Naringin mitigates myocardial strain and the inflammatory response in sepsis-induced myocardial dysfunction through regulation of PI3K/AKT/NF-κB pathway. Int Immunopharmacol. 75:1057822019. View Article : Google Scholar

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Volume 47 Issue 6

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Spandidos Publications style
Li S, Jiang J, Fang J, Li X, Huang C, Liang W and Wu K: Naringin protects H9C2 cardiomyocytes from chemical hypoxia‑induced injury by promoting the autophagic flux via the activation of the HIF‑1α/BNIP3 signaling pathway. Int J Mol Med 47: 102, 2021.
APA
Li, S., Jiang, J., Fang, J., Li, X., Huang, C., Liang, W., & Wu, K. (2021). Naringin protects H9C2 cardiomyocytes from chemical hypoxia‑induced injury by promoting the autophagic flux via the activation of the HIF‑1α/BNIP3 signaling pathway. International Journal of Molecular Medicine, 47, 102. https://doi.org/10.3892/ijmm.2021.4935
MLA
Li, S., Jiang, J., Fang, J., Li, X., Huang, C., Liang, W., Wu, K."Naringin protects H9C2 cardiomyocytes from chemical hypoxia‑induced injury by promoting the autophagic flux via the activation of the HIF‑1α/BNIP3 signaling pathway". International Journal of Molecular Medicine 47.6 (2021): 102.
Chicago
Li, S., Jiang, J., Fang, J., Li, X., Huang, C., Liang, W., Wu, K."Naringin protects H9C2 cardiomyocytes from chemical hypoxia‑induced injury by promoting the autophagic flux via the activation of the HIF‑1α/BNIP3 signaling pathway". International Journal of Molecular Medicine 47, no. 6 (2021): 102. https://doi.org/10.3892/ijmm.2021.4935