Antitumor activity of yulangsan polysacchrides in mice bearing S180 sarcoma tumors

Cai,Wen'e; Chen,Xiaoyu; Pan,Qingdong; Zhang,Shijun; Tan,Luojiao; Sun,Xuyong; Huang,Renbin; Xia,Aijun

doi:10.3892/mco.2017.1378

Antitumor activity of yulangsan polysacchrides in mice bearing S180 sarcoma tumors

Authors:
- Wen'e Cai
- Xiaoyu Chen
- Qingdong Pan
- Shijun Zhang
- Luojiao Tan
- Xuyong Sun
- Renbin Huang
- Aijun Xia
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Affiliations: Department of Clinical Pharmacy, 303 Hospital of PLA, Nanning, Guangxi 530021, P.R. China, Department of Pharmacology, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Diagnosis, Administration of Old Officer of Beiji Temple, Beijing 100191, P.R. China, Guangxi Key Laboratory for Transplantation Medicine, Institute of Transplantation Medicine, 303 Hospital of PLA, Nanning, Guangxi 530021, P.R. China
Published online on: August 16, 2017 https://doi.org/10.3892/mco.2017.1378
Pages: 716-720

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Abstract

Sarcoma is one of the most prevalent pediatric tumors and the therapeutic role of chemotherapy has yet to be elucidated. It has been reported that extracts of Longyanshen (Yulangsan) may enhance the sensitivity of drug‑resistant cancer cell lines, and improve the immune dysfunction induced by cyclophosphamide (CTX) in mice. The present in vivo study investigated the antitumor effects of Yulangsan polysaccharides (YLSPS) and their interaction with CTX in murine sarcoma 180 (S180)‑bearing mice. Immunohistochemistry was used to detect the expression of apoptosis‑related proteins. The ultrastructure of sarcoma cells was examined by transmission electron microscopy and the tumor growth rate was determined by measuring the tumor weight. A dose‑dependent inhibition of sarcoma growth was observed in S180‑bearing mice following administration of YLSPS. In combination with CTX, an additive antitumor effect was obtained, which was accompanied by amelioration of immune function. YLSPS also potentiated the tumor suppression effect of CTX while avoiding cytotoxicity against immune cells. YLSPS inhibited sarcoma growth in S180‑bearing mice through the induction of apoptosis in S180 sarcoma cells. YLSPS also attenuated CTX‑induced cytotoxicity to the immune system while potentiating the tumor suppression effect. These results provide additional information regarding combination therapy with YLSPS and chemotherapy for the treatment of sarcoma.

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