Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells

  • Authors:
    • Hsien‑Kuo Chin
    • Chi‑Ting Horng
    • Yi‑Shan Liu
    • Chi‑Cheng Lu
    • Chen‑Ying Su
    • Pei‑Syuan Chen
    • Hong‑Yi Chiu
    • Fuu‑Jen Tsai
    • Po‑Chuen Shieh
    • Jai‑Sing Yang
  • View Affiliations

  • Published online on: March 14, 2018     https://doi.org/10.3892/or.2018.6312
  • Pages: 2351-2357
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Abstract

Anti-angiogenesis is one of the most general clinical obstacles in cancer chemotherapy. Kaempferol is a flavonoid phytochemical found in many fruits and vegetables. Our previous study revealed that kaempferol triggered apoptosis in human umbilical vein endothelial cells (HUVECs) by ROS‑mediated p53/ATM/death receptor signaling. However, the anti‑angiogenic potential of kaempferol remains unclear and its underlying mechanism warranted further exploration in VEGF‑stimulated HUVECs. In the present study, kaempferol significantly reduced VEGF‑stimulated HUVEC viability. Kaempferol treatment also inhibited cell migration, invasion, and tube formation in VEGF‑stimulated HUVECs. VEGF receptor‑2 (VEGFR‑2), and its downstream signaling cascades (such as AKT, mTOR and MEK1/2‑ERK1/2) were reduced as determined by western blotting and kinase activity assay in VEGF‑stimulated HUVECs after treatment with kaempferol. The present study revealed that kaempferol may possess angiogenic inhibition through regulation of VEGF/VEGFR‑2 and its downstream signaling cascades (PI3K/AKT, MEK and ERK) in VEGF-stimulated endothelial cells.
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May-2018
Volume 39 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chin HK, Horng CT, Liu YS, Lu CC, Su CY, Chen PS, Chiu HY, Tsai FJ, Shieh PC, Yang JS, Yang JS, et al: Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells. Oncol Rep 39: 2351-2357, 2018.
APA
Chin, H., Horng, C., Liu, Y., Lu, C., Su, C., Chen, P. ... Yang, J. (2018). Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells. Oncology Reports, 39, 2351-2357. https://doi.org/10.3892/or.2018.6312
MLA
Chin, H., Horng, C., Liu, Y., Lu, C., Su, C., Chen, P., Chiu, H., Tsai, F., Shieh, P., Yang, J."Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells". Oncology Reports 39.5 (2018): 2351-2357.
Chicago
Chin, H., Horng, C., Liu, Y., Lu, C., Su, C., Chen, P., Chiu, H., Tsai, F., Shieh, P., Yang, J."Kaempferol inhibits angiogenic ability by targeting VEGF receptor-2 and downregulating the PI3K/AKT, MEK and ERK pathways in VEGF-stimulated human umbilical vein endothelial cells". Oncology Reports 39, no. 5 (2018): 2351-2357. https://doi.org/10.3892/or.2018.6312