MicroRNA expression and its detection in human supraglottic laryngeal squamous cell carcinoma

  • Authors:
    • Xin Sun
    • Yan Song
    • Xuhui Tai
    • Bin Liu
    • Wenyue Ji
  • View Affiliations

  • Published online on: July 22, 2013     https://doi.org/10.3892/br.2013.143
  • Pages: 743-746
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Abstract

Laryngeal squamous cell carcinoma (LSCC) is one of the most common head and neck malignancies with a poor prognosis. The aim of this study was to detect the expression of microRNAs (miRNAs) in supraglottic LSCC and attached normal mucosa using microarray assay, and explore the role of miRNAs in supraglottic LSCC. Fresh‑frozen samples of five supraglottic LSCC and attached normal mucosa were obtained, and their various endogenous miRNAs were screened by microarray. miRNAs of interest were confirmed by quantitative real‑time polymerase chain reaction (qRT‑PCR) in 48 supraglottic LSCC tumors and 10 normal mucosa tissues. In total, 38 miRNAs were differentially expressed in supraglottic LSCC, with 22 miRNAs being upregulated and 16 downregulated. Of the six miRNAs selected for qRT‑PCR, miR‑375, miR‑139‑3P, miR‑1290 and miR‑106b were differentially expressed in supraglottic LSCC with and without lymphatic metastasis.
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September-October 2013
Volume 1 Issue 5

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Copy and paste a formatted citation
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Spandidos Publications style
Sun X, Song Y, Tai X, Liu B and Ji W: MicroRNA expression and its detection in human supraglottic laryngeal squamous cell carcinoma. Biomed Rep 1: 743-746, 2013.
APA
Sun, X., Song, Y., Tai, X., Liu, B., & Ji, W. (2013). MicroRNA expression and its detection in human supraglottic laryngeal squamous cell carcinoma. Biomedical Reports, 1, 743-746. https://doi.org/10.3892/br.2013.143
MLA
Sun, X., Song, Y., Tai, X., Liu, B., Ji, W."MicroRNA expression and its detection in human supraglottic laryngeal squamous cell carcinoma". Biomedical Reports 1.5 (2013): 743-746.
Chicago
Sun, X., Song, Y., Tai, X., Liu, B., Ji, W."MicroRNA expression and its detection in human supraglottic laryngeal squamous cell carcinoma". Biomedical Reports 1, no. 5 (2013): 743-746. https://doi.org/10.3892/br.2013.143