Open Access

Probiotic potential of β‑galactosidase‑producing lactic acid bacteria from fermented milk and their molecular characterization

  • Authors:
    • Mahanthesh Vasudha
    • Chakra S Prashantkumar
    • Mallika Bellurkar
    • Vishwas Kaveeshwar
    • Devaraja Gayathri
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  • Published online on: February 8, 2023     https://doi.org/10.3892/br.2023.1605
  • Article Number: 23
  • Copyright: © Vasudha et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Probiotics have attained significant interest in recent years as a result of their gut microbiome modulation and gastrointestinal health benefits. Numerous fermented foods contain lactic acid bacteria (LAB) which are considered as GRAS and probiotic bacteria. The present study aimed to investigate indigenous LAB from homemade fermented milk samples collected in remote areas of Karnataka (India), in order to isolate the most potent and well‑adapted to local environmental conditions bacteria, which were then evaluated using a step‑by‑step approach focused on the evaluation of probiotic traits and β‑galactosidase‑producing ability. LAB were screened using 5‑bromo‑4‑chloro‑3‑indole‑D‑­galactopyranoside (X‑Gal) and O‑nitrophenyl‑β‑D‑galactopyranoside (ONPG) as substrate, and exhibited β‑galactosidase activity ranging from 728.25 to 1,203.32 Miller units. The most promising isolates were selected for 16S rRNA gene sequence analysis and identified as Lactiplantibacillus plantarum, Limosilactobacillus fermentum, Lactiplantibacillus pentosus and Lactiplantibacillus sp. Furthermore, these isolates were evaluated by in vitro, viz., survival in gastrointestinal tract, antibiotic susceptibility, antimicrobial activity, cell surface characteristics, and haemolytic activity. All eight isolates demonstrated strong adherence and prevented pathogen penetration into HT‑29 cells, indicating potential of the bacteria to scale up industrial level production of milk products for lactose intolerants.
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March-2023
Volume 18 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Vasudha M, Prashantkumar CS, Bellurkar M, Kaveeshwar V and Gayathri D: Probiotic potential of β‑galactosidase‑producing lactic acid bacteria from fermented milk and their molecular characterization. Biomed Rep 18: 23, 2023.
APA
Vasudha, M., Prashantkumar, C.S., Bellurkar, M., Kaveeshwar, V., & Gayathri, D. (2023). Probiotic potential of β‑galactosidase‑producing lactic acid bacteria from fermented milk and their molecular characterization. Biomedical Reports, 18, 23. https://doi.org/10.3892/br.2023.1605
MLA
Vasudha, M., Prashantkumar, C. S., Bellurkar, M., Kaveeshwar, V., Gayathri, D."Probiotic potential of β‑galactosidase‑producing lactic acid bacteria from fermented milk and their molecular characterization". Biomedical Reports 18.3 (2023): 23.
Chicago
Vasudha, M., Prashantkumar, C. S., Bellurkar, M., Kaveeshwar, V., Gayathri, D."Probiotic potential of β‑galactosidase‑producing lactic acid bacteria from fermented milk and their molecular characterization". Biomedical Reports 18, no. 3 (2023): 23. https://doi.org/10.3892/br.2023.1605