Open Access

Indocyanine green uptake by human tumor and non‑tumor cell lines and tissue

  • Authors:
    • Hoang-Ngan Nguyen
    • David Pertzborn
    • Rafat Ziadat
    • Günther Ernst
    • Orlando Guntinas‑Lichius
    • Ferdinand Von Eggeling
    • Franziska Hoffmann
  • View Affiliations

  • Published online on: July 26, 2024     https://doi.org/10.3892/br.2024.1824
  • Article Number: 136
  • Copyright: © Nguyen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].

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Abstract

Indocyanine green (ICG) is a potential promising dye for a better intraoperative tumor border definition and an improved patient outcome by potentially improving tumor border visualization compared with traditional white light guided surgery. Here, the cellular uptake of ICG in human squamous cell carcinoma (SCC026) and immortalized non‑cancer skin (HaCaT) cell lines was evaluated to study the tumor‑specific cellular uptake of ICG. The spatial distribution of ICG inside tumor tissue was investigated in tissue sections of head and neck squamous cell carcinoma at a microscopic level. ICG uptake and internalization was observed in living cells after 2.5 h and in the nucleus after 24 h. In dead cells, higher and faster uptake was observed. In the tissue sections, higher ICG signal intensity could be detected in connective tissue and surrounding clusters and blood vessels. In conclusion, no distinct ICG uptake by tumor cells was detected in cancer cell lines and tumor tissue. ICG localization in certain regions of tumor tissue appears to be a result of enhanced tissue permeability and retention, but not specific to tumor cells.
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September-2024
Volume 21 Issue 3

Print ISSN: 2049-9434
Online ISSN:2049-9442

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Spandidos Publications style
Nguyen H, Pertzborn D, Ziadat R, Ernst G, Guntinas‑Lichius O, Von Eggeling F and Hoffmann F: Indocyanine green uptake by human tumor and non‑tumor cell lines and tissue. Biomed Rep 21: 136, 2024.
APA
Nguyen, H., Pertzborn, D., Ziadat, R., Ernst, G., Guntinas‑Lichius, O., Von Eggeling, F., & Hoffmann, F. (2024). Indocyanine green uptake by human tumor and non‑tumor cell lines and tissue. Biomedical Reports, 21, 136. https://doi.org/10.3892/br.2024.1824
MLA
Nguyen, H., Pertzborn, D., Ziadat, R., Ernst, G., Guntinas‑Lichius, O., Von Eggeling, F., Hoffmann, F."Indocyanine green uptake by human tumor and non‑tumor cell lines and tissue". Biomedical Reports 21.3 (2024): 136.
Chicago
Nguyen, H., Pertzborn, D., Ziadat, R., Ernst, G., Guntinas‑Lichius, O., Von Eggeling, F., Hoffmann, F."Indocyanine green uptake by human tumor and non‑tumor cell lines and tissue". Biomedical Reports 21, no. 3 (2024): 136. https://doi.org/10.3892/br.2024.1824