Open Access

Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism

  • Authors:
    • Liya Zhang
    • Kunpeng Wu
    • Tao Bo
    • Lingyan Zhou
    • Ling Gao
    • Xiaoming Zhou
    • Wenbin Chen
  • View Affiliations

  • Published online on: December 4, 2019     https://doi.org/10.3892/etm.2019.8281
  • Pages: 897-906
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Subclinical hypothyroidism (SCH) is becoming a global health problem due to its increasing prevalence and potential adverse effects, including cardiovascular diseases and nonalcoholic fatty liver disease (NAFLD). However, the association between SCH and NAFLD remains controversial. MicroRNAs (miRNAs/miRs) have been reported to be implicated in lipid metabolism disorders; however, how miRNAs regulate hepatic lipid metabolism in SCH mice remains unknown. The present study investigated miRNA alterations and proteome profiles in an SCH mouse model, which was generated by methimazole administration in mice for 16 weeks. Next, the profiles of 17 miRNAs that are critical to hepatic lipid metabolism and the proteome were investigated using reverse transcription‑quantitative polymerase chain reaction and iTRAQ labeling in the liver specimens of SCH (n=9) and control (n=7) mice. Putative target prediction of miRNAs was also conducted using TargetScan and miRanda. Compared with the control mice, SCH mice had 8 miRNAs and 36 proteins with significantly different expression in the liver tissues. Furthermore, a regulatory module containing 3 miRNAs (miR‑34a‑5p, miR‑24‑3p and miR‑130a‑3p) and 4 proteins (thioredoxin, selenium‑binding protein 2, elongation factor 1β and prosaposin) was identified. Overall, integrated analysis of miRNAs and the proteome highlighted a regulatory module between miRNAs and proteins, which, to a certain extent, may contribute to a better understanding of hepatic lipid metabolism disorders in SCH mice.
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February-2020
Volume 19 Issue 2

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Zhang L, Wu K, Bo T, Zhou L, Gao L, Zhou X and Chen W: Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism. Exp Ther Med 19: 897-906, 2020.
APA
Zhang, L., Wu, K., Bo, T., Zhou, L., Gao, L., Zhou, X., & Chen, W. (2020). Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism. Experimental and Therapeutic Medicine, 19, 897-906. https://doi.org/10.3892/etm.2019.8281
MLA
Zhang, L., Wu, K., Bo, T., Zhou, L., Gao, L., Zhou, X., Chen, W."Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism". Experimental and Therapeutic Medicine 19.2 (2020): 897-906.
Chicago
Zhang, L., Wu, K., Bo, T., Zhou, L., Gao, L., Zhou, X., Chen, W."Integrated microRNA and proteome analysis reveal a regulatory module in hepatic lipid metabolism disorders in mice with subclinical hypothyroidism". Experimental and Therapeutic Medicine 19, no. 2 (2020): 897-906. https://doi.org/10.3892/etm.2019.8281