Open Access

miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1

  • Authors:
    • Yuxia Li
    • Jun Zhuang
  • View Affiliations

  • Published online on: November 2, 2020     https://doi.org/10.3892/etm.2020.9434
  • Article Number: 2
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recent studies have demonstrated that microRNAs (miRs) serve a crucial role during the development of gestational diabetes mellitus (GDM). However, the mechanisms underlying miR‑345‑3p and its protective role during GDM have not been previously reported. The present study investigated miR‑345‑3p expression and function in vitro, and the possible molecular mechanisms underlying GDM. Compared with healthy pregnant women, miR‑345‑3p was downregulated in the placental tissue and peripheral blood of patients with GDM. Further investigation revealed that BCL2‑antagonist/killer 1 (BAK1) was a predicted target gene of miR‑345‑3p, and the expression of BAK1 was significantly increased in patients with GDM compared with healthy pregnant women. In vitro analysis revealed that miR‑345‑3p mimic significantly increased cell viability, migration and invasion, inhibited apoptosis, upregulated Bcl‑2 and matrix metallopeptidase 9 expression, and decreased Bax expression compared with the control group. Furthermore, miR‑245‑3p mimic‑induced alterations were reversed by BAK1 overexpression. The results suggested that miR‑345‑3p overexpression exhibited a protective role in patients with GDM by inhibiting HTR8‑/SVneo cell apoptosis, and promoting cell proliferation and migration via targeting BAK1. The use of miR‑345‑3p for the diagnosis of GDM requires further investigation.
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January-2021
Volume 21 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Li Y and Li Y: miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1. Exp Ther Med 21: 2, 2021
APA
Li, Y., & Li, Y. (2021). miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1. Experimental and Therapeutic Medicine, 21, 2. https://doi.org/10.3892/etm.2020.9434
MLA
Li, Y., Zhuang, J."miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1". Experimental and Therapeutic Medicine 21.1 (2021): 2.
Chicago
Li, Y., Zhuang, J."miR‑345‑3p serves a protective role during gestational diabetes mellitus by targeting BAK1". Experimental and Therapeutic Medicine 21, no. 1 (2021): 2. https://doi.org/10.3892/etm.2020.9434