Open Access

Sirtuin 5 alleviates apoptosis and autophagy stimulated by ammonium chloride in bovine mammary epithelial cells

  • Authors:
    • Junhui He
    • Luping Feng
    • Hanlin Yang
    • Shikai Gao
    • Jinru Dong
    • Guangyang Lu
    • Luya Liu
    • Xinyi Zhang
    • Kai Zhong
    • Shuang Guo
    • Guangming Zha
    • Liqiang Han
    • Heping Li
    • Yueying Wang
  • View Affiliations

  • Published online on: May 22, 2024     https://doi.org/10.3892/etm.2024.12584
  • Article Number: 295
  • Copyright: © He et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ammonia (NH3) is an irritating and harmful gas that affects cell apoptosis and autophagy. Sirtuin 5 (SIRT5) has multiple enzymatic activities and regulates NH3‑induced autophagy in tumor cells. In order to determine whether SIRT5 regulates NH3‑induced bovine mammary epithelial cell apoptosis and autophagy, cells with SIRT5 overexpression or knockdown were generated and in addition, bovine mammary epithelial cells were treated with SIRT5 inhibitors. The results showed that SIRT5 overexpression reduced the content of NH3 and glutamate in cells by inhibiting glutaminase activity in glutamine metabolism, and reduced the ratio of ADP/ATP. The results in the SIRT5 knockdown and inhibitor groups were comparable, including increased content of NH3 and glutamate in cells by activating glutaminase activity, and an elevated ratio of ADP/ATP. It was further confirmed that SIRT5 inhibited the apoptosis and autophagy of bovine mammary epithelial cells through reverse transcription‑quantitative PCR, western blot, flow cytometry with Annexin V FITC/PI staining and transmission electron microscopy. In addition, it was also found that the addition of LY294002 or Rapamycin inhibited the PI3K/Akt or mTOR kinase signal, decreasing the apoptosis and autophagy activities of bovine mammary epithelial cells induced by SIRT5‑inhibited NH3. In summary, the PI3K/Akt/mTOR signal involved in NH3‑induced cell autophagy and apoptosis relies on the regulation of SIRT5. This study provides a new theory for the use of NH3 to regulate bovine mammary epithelial cell apoptosis and autophagy, and provides guidance for improving the health and production performance of dairy cows.
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July-2024
Volume 28 Issue 1

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
He J, Feng L, Yang H, Gao S, Dong J, Lu G, Liu L, Zhang X, Zhong K, Guo S, Guo S, et al: Sirtuin 5 alleviates apoptosis and autophagy stimulated by ammonium chloride in bovine mammary epithelial cells. Exp Ther Med 28: 295, 2024
APA
He, J., Feng, L., Yang, H., Gao, S., Dong, J., Lu, G. ... Wang, Y. (2024). Sirtuin 5 alleviates apoptosis and autophagy stimulated by ammonium chloride in bovine mammary epithelial cells. Experimental and Therapeutic Medicine, 28, 295. https://doi.org/10.3892/etm.2024.12584
MLA
He, J., Feng, L., Yang, H., Gao, S., Dong, J., Lu, G., Liu, L., Zhang, X., Zhong, K., Guo, S., Zha, G., Han, L., Li, H., Wang, Y."Sirtuin 5 alleviates apoptosis and autophagy stimulated by ammonium chloride in bovine mammary epithelial cells". Experimental and Therapeutic Medicine 28.1 (2024): 295.
Chicago
He, J., Feng, L., Yang, H., Gao, S., Dong, J., Lu, G., Liu, L., Zhang, X., Zhong, K., Guo, S., Zha, G., Han, L., Li, H., Wang, Y."Sirtuin 5 alleviates apoptosis and autophagy stimulated by ammonium chloride in bovine mammary epithelial cells". Experimental and Therapeutic Medicine 28, no. 1 (2024): 295. https://doi.org/10.3892/etm.2024.12584