The anti-obesity effects of a tuna peptide on 3T3-L1 adipocytes are mediated by the inhibition of the expression of lipogenic and adipogenic genes and by the activation of the Wnt/β-catenin signaling pathway

Kim,Young‑Min; Kim,In‑Hye; Choi,Jeong‑Wook; Lee,Min‑Kyeong; Nam,Taek‑Jeong

doi:10.3892/ijmm.2015.2231

The anti-obesity effects of a tuna peptide on 3T3-L1 adipocytes are mediated by the inhibition of the expression of lipogenic and adipogenic genes and by the activation of the Wnt/β-catenin signaling pathway

Authors:
- Young‑Min Kim
- In‑Hye Kim
- Jeong‑Wook Choi
- Min‑Kyeong Lee
- Taek‑Jeong Nam
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Affiliations: Department of Food Science and Nutrition, Pukyong National University, Busan 608‑737, Republic of Korea
Published online on: June 3, 2015 https://doi.org/10.3892/ijmm.2015.2231
Pages: 327-334
Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

The differentiation of 3T3-L1 cells into adipocytes involves the activation of an organized system of obesity-related genes, of which those encoding CCAAT/enhancer‑binding proteins (C/EBPs) and the Wnt-10b protein may play integral roles. In a previous study of ours, we found that a specific peptide found in tuna (sequence D‑I‑V‑D‑K‑I‑E‑I; termed TP‑D) inhibited 3T3‑L1 cell differentiation. In the present study, we observed that the expression of expression of C/EBPs and Wnt‑10b was associated with obesity. The initial step of 3T3‑L1 cell differentiation involved the upregulation of C/EBP‑α expression, which in turn activated various subfactors. An upstream effector of glycogen synthase kinase-3β (GSK‑3β) inhibited Wnt‑10b expression in 3T3‑L1 adipocytes. In a previous study of ours, we sequenced the tuna peptide via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS‑PAGE) and quadrupole time-of-flight mass spectrometry (Q-TOF MS/MS) and confirmed the anti‑obesity effects thereof in 3T3‑L1 adipocytes. In the present study, we demonstrate that TP‑D inhibits C/EBP and promotes Wnt‑10b mRNA expression, thus activating the Wnt pathway. The inhibition of lipid accumulation was measured using a glucose and triglyceride (TG) assay. Our results confirmed that TP‑D altered the expression levels of C/EBP‑related genes in a dose‑dependent manner and activated the Wnt signaling pathway. In addition, we confirmed that total adiponectin and high‑molecular weight (HMW) adiponectin levels were reduced by treatment with TP‑D. These data indicate that TP‑D inhibits adipocyte differentiation through the inhibition of C/EBP genes and the subsequent activation of the Wnt/β-catenin signaling pathway.

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