Open Access

Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold

  • Authors:
    • Ji Bao
    • Qiong Wu
    • Yujia Wang
    • Yi Li
    • Li Li
    • Fei Chen
    • Xiujuan Wu
    • Mingjun Xie
    • Hong Bu
  • View Affiliations

  • Published online on: June 14, 2016     https://doi.org/10.3892/ijmm.2016.2638
  • Pages: 457-465
  • Copyright: © Bao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

In the present study, we aimed to determine whether the combination of aggregate culture and decellularized liver scaffolds (DLSs) promoted the hepatic differentiation of murine bone marrow-derived mesenchymal stem cells (BM-MSCs) into high yields of mature hepatocytes in vitro. Four culturing methods for differentiation [single cell (2D), spheroids (3D), 2D + DLS and 3D + DLS] were studied. To determine the differentiation stages of the MSCs, RT-qPCR of the hepatocyte genes, immunostaining of hepatocyte markers, and functional analyses were all performed. Compared with the other groups, hepatocyte-like cells which differentiated from BM‑MSC spheroids on extracellular matrix (ECM) exhibited more intensive staining of stored glycogen, an elevated level of urea biosynthesis and albumin secretion as well as the higher expression of hepatocyte-specific genes. Our results indicated that DLSs combined with spheroidal aggregate culture may be used as an effective method to facilitate the hepatic maturation of BM-MSCs and may have future applications in stem cell-based liver regenerative medicine.
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August-2016
Volume 38 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Bao J, Wu Q, Wang Y, Li Y, Li L, Chen F, Wu X, Xie M and Bu H: Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold. Int J Mol Med 38: 457-465, 2016.
APA
Bao, J., Wu, Q., Wang, Y., Li, Y., Li, L., Chen, F. ... Bu, H. (2016). Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold. International Journal of Molecular Medicine, 38, 457-465. https://doi.org/10.3892/ijmm.2016.2638
MLA
Bao, J., Wu, Q., Wang, Y., Li, Y., Li, L., Chen, F., Wu, X., Xie, M., Bu, H."Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold". International Journal of Molecular Medicine 38.2 (2016): 457-465.
Chicago
Bao, J., Wu, Q., Wang, Y., Li, Y., Li, L., Chen, F., Wu, X., Xie, M., Bu, H."Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold". International Journal of Molecular Medicine 38, no. 2 (2016): 457-465. https://doi.org/10.3892/ijmm.2016.2638