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Sarcolemmal ATP-sensitive potassium channel protects cardiac myocytes against lipopolysaccharide-induced apoptosis
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- Published online on: July 5, 2016 https://doi.org/10.3892/ijmm.2016.2664
- Pages: 758-766
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Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Sepsis, also known as systemic inflammatory response syndrome (SIRS), is caused by infection associated with organ damage. Sepsis is a life-threatening condition characterized by rapid progression and a high fatality rate. The mortality rate of sepsis is complicated by heart failure and may reach 70–90% (1). Cardiac dysfunction in sepsis is a complex pathophysiological process and the mechanisms underlying sepsis-induced myocardial dysfunction remain to be elucidated. Pro-inflammatory cytokines (such as tumor necrosis factor (TNF)-α and interleukin (IL)-1), endothelin-1, nitric oxide and adhesion molecules act directly or indirectly to suppress cardiac function in sepsis, which results in myocardial dysfunction (2).
Apoptosis has been reported to play an important role in sepsis-induced cardiac dysfunction (3). The pharmacological inhibition of cardiac apoptosis prevents sepsis-induced myocardial dysfunction (4). Although a number of mediators and pathways have been associated with apoptosis which occurs in septic myocardial dysfunction, the precise cause remains elusive.
The ATP-sensitive K+ (KATP) channels, first discovered in the heart more than 20 years ago, represent a unique group of channels that are regulated predominantly by the cellular metabolic state (5). The function of the cardiac sarcolemmal KATP (sarcKATP) channel was initially associated with the cardiac response to stress (5), and this was later confirmed by a number of studies. The opening of sarcKATP channels under conditions of metabolic stress affects excitability and other membrane potential-related functions, such as Ca2+ loading, thus, helping to maintain cellular homeostasis during cardiac challenge (5). Studies suggest that the opening of KATP channels prevents activation of the inflammatory process and the production of a variety of pro-inflammatory factors in microglial cells (6), and blocks the apoptosis of cardiocytes in ischemia-reperfusion (7). KATP channels also play an essential role in the cardiovascular adaptive response during stress (8). Furthermore, Buckley et al proposed that the opening of KATP channels may actually represent a protective mechanism against cellular damage in endotoxemia (9). Several researchers have reported that KATP channels open in sepsis (10–12); however, whether or not they exert a regulatory effect on the apoptosis of septic myocytes has yet to be determined. It is well established that sepsis releases lipopolysaccharide (LPS) into the circulation. LPS exerts a deleterious effect on cardiac function and plays a significant role in the development of acute and chronic heart failure (13). In the present study, we examined the role of cardiac sarcKATP channels in the LPS-induced apoptosis of cultured neonatal rat cardiomyocytes (NRCs). Furthermore, we identified the downstream effects of cardiac sarcKATP channel inhibition and activation by focusing on the interaction between the sarcKATP channel and mitochondrial calcium.
Materials and methods
Animals
The animal studies were conducted in accordance with the guidelines of the Experimental Animal Center of Guangdong Province (Guangzhou, China). This study was approved by the Ethics Committee of Guangzhou University of Traditional Chinese Medicine (Shenzhen, China). The rats were housed in a temperature- and humidity-controlled room under a 12-h light/dark cycle prior to the beginning of the experiments. No anesthetics were administered in order to avoid interference with biochemical values.
Reagents
LPS from Escherichia coli serotype 055:B5 was purchased from Sigma-Aldrich (St. Louis, MO, USA). A terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) kit was purchased from Roche (Mannheim, Germany). Assay kits for the determination of caspase-3 activity were purchased from Beyotime Institute of Biotechnology (Haimen, China).
Cultured NRCs
Primary cultures of NRCs were prepared from the ventricles of 1–3-day-old Sprague-Dawley rats, as described previously (14), with some modifications. Briefly, the neonatal rats were decapitated, the hearts were excised, and ventricular myocardium was sectioned into 1 mm3-thick slices and incubated with 0.25% trypsin (3–5 ml) in a shaker at 37°C for fractionated digestion. The tissue pieces were allowed to settle, and all the supernatant was collected, and centrifuged at 1,000 × g for 10 min. The supernatant was discarded, and a single cell suspension was obtained with Dulbecco's modified Eagle's medium (DMEM) containing 20% fetal bovine serum (FBS). Ventricular myocytes were separated from the faster-attaching nonmyocytes. The ventricular myocytes in the supernatant were collected and plated on a 12-well culture plate. The NRCs were used for experiments following a demonstration of confluence and rhythmic contraction after 72 h. To explore the roles of sarcKATP channel and mitochondrial calcium in the LPS-induced apoptosis of myocytes, the following activators and blockers were used: sarcKATP channel opener (P-1075, 100 µM), sarcKATP channel blocker (HMR-1098, 30 µM) and mitochondrial Ca2+ uniporter inhibitor [ruthenium red (RR), 50 µM], respectively. They were applied following stimulation with 25 µg/ml LPS or vehicle for 24 h. The negative control included cells maintained in DMEM containing 10% FBS with or without inhibitors and not exposed to LPS challenge.
Analysis of cardiomyocyte viability
The cytotoxic effects of LPS on cardiac myocytes were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and the optimal exposure time and dose of LPS was established. Exogenous MTT was reduced to insoluble purple crystal sediment, which dissolves in dimethyl sulfoxide (DMSO), within the cells by mitochondrial succinate dehydrogenase in the viable cells, but not in the dead cells. The cells were seeded in 96-well plates at a density of 5×104 cells/well. The cardiomyocytes were incubated with 20 µl MTT solution (5 mg/ml; HyClone, Logan, UT, USA) for 4 h at 37°C. Next, 150 µl DMSO (HyClone) was added to each well to dissolve the formazan crystals, and the plate was agitated for 10 min until all the crystals were dissolved. The amount of MTT formazan was quantified by determining the absorbance at 570 nm using a microplate reader (ELX808; Biotek, Winooski, VT, USA). The viability was calculated as follows: viability (%) = (A570, sample-A570, blank)/(A570, control-A570, blank) ×100.
Assessment of apoptosis by TUNEL assay
Apoptosis was analyzed by TUNEL assay (Roche) and Hoechst 33258 staining (H1399; Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. The TUNEL assay was used in order to detect DNA strand breaks. Briefly, the NRCs were grown on laminin-coated chamber slides and exposed to LPS challenge as described above. Twenty-four hours later, the cells were fixed in 4% paraformaldehyde (PFA) for 1 h, and the TUNEL assay was performed, according to the manufacturer's instructions using a commercial kit (Roche). Apoptotic nuclei stained with fluorescein isothiocyanate (FITC; TUNEL assay) were visualized by confocal microscopy (FV1000; Olympus, Tokyo, Japan). TO-PRO®-3 stain was used to stain all nuclei. TUNEL-positive cells were counted and the number of apoptotic cells was expressed as a percentage of the total number of cells.
Expression of recombinant adenovirus vector containing subunits of KATP channel mutant Kir6.2AAA in primary cultured NRCs
A recombinant adenovirus vector carrying KATP channel mutant subunit Kir6.2AAA was constructed and expressed in rat cardiomyocytes. Based upon primers for Kir6.2 sites, site-directed mutagenesis of Kir6.2 GFG amino acids into AAA was performed by means of overlap polymerase chain reaction (PCR). PCR products were cloned into a pShuttle vector for sequence analysis. After PmeI linearization, it was transformed into the adenovirus expression vector pAdEasy-1. The pAdEasy-1 vector was then packaged into a liposome and transfected into primary cultured rat cardiomyocytes. The green fluorescent protein (GFP)-expressing vector (Invitrogen, Shanghai, China) was used as a control. The expression of Kir6.2AAA was confirmed by RT-PCR and western blot analysis. The effects on the LPS-induced apoptosis of myocytes were observed in the presence or absence of LPS.
Caspase-3 activity
To measure caspase-3 enzymatic activity, the NRCs were cultured for 48 h followed by treatment with P-1075 (100 µM), HMR-1098 (30 µM) and/or RR (50 µM) for 30 min following treatment with or without LPS (25 µg/ml). The activity of caspase-3 was determined using a caspase assay kit (Beyotime Institute of Biotechnology) based on the ability of caspase-3 to change acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) into a yellow formazan product p-nitroaniline (pNA). Protein concentrations were determined using a Bradford protein assay (Bio-Rad, Hercules, CA, USA).
Western blot analysis
Cultured myocytes were harvested and lysed for 30 min on ice in lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% NP-40, 10% glycerol, 0.4 mM sodium orthovanadate, 10 mM sodium pyrophosphate, 10 mM sodium fluoride, 0.5 mM dithiothreitol (DTT) and 2 µl/ml protease inhibitor). Following 15 min of centrifugation at 12,000 × g and 4°C, the supernatants were obtained and used in subsequent experiments. Equal amounts of protein from each sample were resolved on sodium dodecyl sulfate (SDS)-polyacrylamide gel by electrophoresis, and transferred to Immobilon polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA), blocked with 5% BSA in TBST (20 mM Tris-HCl, 137 mM NaCl, and 0.1% Tween-20, pH 7.5) at room temperature for 1 h. The membranes were incubated with primary antibodies overnight at 4°C. Following incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3,000; Forevergen, Guangzhou, China), the immunoblots were exposed to enhanced chemiluminescence (ECL) reagent (Forevergen). The bands were quantified by optical density using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as a control. The primary antibodies were as follows: anti-Bcl-2 (CST2870; 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-Bax (SC-493; 1:200; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and anti-GAPDH (HC301; 1:5,000; TransGen Biotech, Beijing, China).
Determination of apoptosis by flow cytometry
The apoptotic ratio was determined by flow cytometry with Annexin V-FITC/PI staining, according to manufacturer's instructions. Briefly, the NRCs were treated with P-1075 (100 µM), HMR-1098 (30 µM) and/or RR (50 µM) for 24 h in the presence or absence of LPS (25 µg/ml). Following experimental treatment, the cells were collected, washed with calcium-free phosphate-buffered saline (PBS) and resuspended in binding buffer. The cells were treated with Annexin V-FITC and PI, left in the dark at room temperature for 15 min, and analyzed using a Beckton-Dickinson flow cytometer (Navios; Beckman Coulter, Brea, CA, USA) [fluorescence-activated cell sorting (FACS) analysis].
Statistical analysis
Average values are presented in histograms and in the text as the means ± SD. Data were analyzed using SPSS 11.5 for Windows (SPSS, Inc., Chicago, IL, USA). The total number of cells analyzed in each condition is given in the figure legends. The t test was used to determine statistical significance. P-values in the figures are represented by asterisks.
Results
NRC culture and LPS exposure
After continuous culture for 24 h, all adherent cardiac myocytes were spindle-, diamond- or polygonal-shaped and spontaneously beating at 60–100 beats/min. The cells migrated across the surface and developed pseudopodia, forming irregular star shapes and weaving into a network of mutual contacts (data not shown). After 72 h, the cultured cells were arranged in radial clusters (Fig. lA) beating synchronously and autonomously at a rate of 100 beats/min. After stable growth for 24–48 h, the cardiomyocytes were exposed to different concentrations of LPS (0, 5, 25 or 50 µg/ml) for various time periods (0, 4, 8 and 24 h) in order to determine the optimal observation window. The viability of the cardiomyocytes was determined using the MTT method, for the optimal dose and time duration of LPS exposure. We found that LPS induced cardiomyocyte death in a time- and concentration-dependent manner. Treating the myocytes with 25 µg/ml LPS for 24 h resulted in a nearly 60% survival rate (Fig. 1B), which was an appropriate observation window for the follow-up experiments.
Effects of sarcKATP channel and mitochondrial calcium on the viability and LPS-induced apoptosis of NRCs
To determine whether further activation or inhibition of the sarcKATP channel affected the viability and LPS-induced apoptosis of NRCs, the cells were exposed to 25 µg/ml LPS for 24 h followed by treatment with 100 µM P-1075, 30 µM HMR-1098 and 50 µM RR.
Cellular viability is an important indicator of NRC injury. Following LPS exposure, cellular viability was 62.6±4.0%, and significantly reduced compared with the control group (P<0.01). P-1075 markedly increased cellular viability to 71.3±7.1% (P<0.05, compared with the LPS group). HMR-1098 reduced cellular viability to 42.8±6.3% (P<0.01, compared with the LPS group). These results indicated that the sarcKATP channel significantly preserved cellular viability (Fig. 1C).
Fig. 2A shows the TUNEL and Hoechst staining images of the NRCs. As shown in the bar graph in Fig. 2B, there was a significant increase in apoptosis in the LPS group compared with that in the control group (40.1±6.7% in the LPS group vs. 8.7±2.6% in the control group, P<0.01). The percentage of apoptotic cells was further increased in the presence of HMR-1098 (69.3±6.9% in the LPS + HMR group, P<0.01 compared with the LPS group). The percentage of apoptotic cells was decreased in the presence of P-1075 (24.3±6.6% in the LPS + P group, P<0.01 compared with LPS group). The application of RR resulted in the attenuation of LPS-induced apoptosis in the presence of HMR-1098 (43.3±2.4% in the LPS + RR group and 54.7±6.2% in the LPS + HMR + RR group, respectively, P<0.01 vs. LPS + HMR group) and P-1075 (43.3±2.4% in the LPS + RR group and 37.0±2.4%).
Effects of sarcKATP channel and mitochondrial calcium on caspase-3 activity in NRCs
LPS-induced apoptosis of cardiomyocytes is multifactorial. We examined the activity of caspase-3 in the NRCs. As shown in Fig. 3, caspase-3 activity was markedly higher 24 h after LPS stimulation in the LPS group compared with that in the control group (P<0.01). Caspase-3 activity was suppressed markedly in the LPS + P-1075 group compared with the LPS group (P<0.01). Caspase-3 activity was significantly increased in the LPS + HMR-1098 group (P<0.01, compared with LPS group), which was partly attenuated in the presence of 50 µM RR (P<0.01, compared with the LPS group).
Effects of sarcKATP channel and mitochondrial calcium on the expression of the apoptosis-related proteins Bcl-2 and Bax
In order to determine whether the sarcKATP channel modulates some of the anti- and pro-apoptotic regulators, the protein levels of Bcl-2 and Bax were assessed in the present study by western blot analysis (Fig. 4A). As also shown in Fig. 4B and C, LPS stimulus downregulated Bcl-2 expression and upregulated Bax expression, and P-1075 increased the Bcl-2 protein level and decreased the Bax protein level in the LPS-exposed NRCs. By contrast, HMR-1098 decreased the Bcl-2 protein level and increased the Bax protein level in the LPS-exposed NRCs. The results suggested that sarcKATP prevented the apoptosis of NRCs by increasing Bcl-2 expression and inhibiting Bax expression (Fig. 4)
Effect of Kir6.2AAA overexpression on LPS-induced apoptosis
The recombinant adenovirus carrying the gene fragment Kir6.2AAA and GFP was constructed successfully, with a viral titer of 2.64×1011 VP/ml. Following infection with recombinant adenovirus Kir6.2AAA, the rat myocytes expressed GFP and emitted green fluorescence (Fig. 5A). The expression of Kir6.2AAA was significantly upregulated in recombinant adenovirus vector Ad-Kir6.2AAA-infected myocytes and this was confirmed by RT-PCR (data not shown). The increased expression of Kir6.2AAA was confirmed in rat myocytes by western blot analysis, which suggested that the recombinant adenovirus carrying the gene fragment Kir6.2AAA and GFP was constructed successfully and expressed correctly in the rat myocytes (data not shown).
Adenovirus infection has little effect on cellular viability. Following LPS exposure, cellular viability of the GFP + LPS group was significantly reduced (72.1±3.6%) compared with the GFP group (P<0.01). Overexpression of Kir6.2AAA (Kir6.2 group) markedly reduced the cellular viability to 43.4±2.8% (P<0.01, compared with GFP + LPS group). RR treatment increased the cellular viability to 62.0±6.9% (P<0.05, compared with GFP + LPS group) (Fig. 5B).
Apoptosis was measured by flow cytometry (Fig. 7A). Adenovirus infection had little effect on myocardial apoptosis: apoptotic rate of the Ad-GFP group was 3.4±0.7%; apoptotic rate of the Ad-Kir6.2AAA group was 3.7±0.7%; apoptotic rate of the Ad-Kir6.2AAA + RR group was 4.1±0.6%. The apoptotic rate of the myocytes significantly increased following LPS exposure: apoptotic rate of the Ad-GFP + LPS group was 14.9±2.8%; apoptotic rate of the Ad-Kir6.2AAA + LPS was 36.0±3.8%; apoptotic rate of the Ad-Kir6.2AAA + RR + LPS group was 19.4±3.7% (Fig. 7B). The results suggested that in the model of LPS-induced apoptosis in NRCs, overexpression of Kir6.2AAA further promoted the apoptosis of myocytes, and RR treatment protected the myocytes from LPS-induced apoptosis.
The effect of Kir6.2AAA overexpression on Bcl-2 and Bax protein levels was detected by western blot analysis (Fig. 6A). Our results showed that adenovirus infection has no effect on the protein expression of Bcl-2 and Bax. LPS downregulated Bcl-2 expression and upregulated Bax expression in the GFP + LPS group. Overexpression of Kir6.2AAA (Kir6.2 group) markedly decreased the Bcl-2 protein level and increased the Bax protein level in the LPS-exposed NRCs (Fig. 6B and C). RR treatment increased the Bcl-2 protein level and decreased the Bax protein level in the LPS-exposed NRCs (Fig. 6).
Discussion
In the present study, we examined the role of the sarcKATP channel in the LPS-induced apoptosis of NRCs. Our findings suggest that the sarcKATP channel exerts a cardioprotective effect against apoptosis induced by LPS and is mediated by mitochondrial Ca2+ in rat cardiomyocytes.
The protective role of the sarcKATP channels has been proposed since the time of their discovery due to their ability to sense intracellular metabolic conditions (15). In 1983, Noma (5) identified a KATP channel in membrane patches prepared from isolated guinea pig ventricular myocytes. Subsequently, KATP channels were discovered in other tissues, including the brain, smooth muscle, skeletal muscle, intestine, kidney and pancreas. The channel coupled myocardial energy metabolism to membrane electrochemistry, which highlighted its role in all types of ion channels. The metabolic gating of the sarcKATP channel is attributed to its molecular composition. Structurally, KATP channels are composed of two distinct proteins, an inwardly rectifying potassium channel (Kir) pore subunit and the sulfonylurea receptor (SUR), which may have a regulatory role in modulating the sensitivity of the channel to ATP, other nucleotides, and pharmacological agonists or antagonists (16). The cardiac sarcolemmal KATP channel is composed of an octomeric complex of two types of subunits, the Kir6.2 and the SUR2A subunit.
The cardiac sarcKATP channel is the sensory receptor of cellular energy metabolism and highly sensitive to fluctuations in its microenvironment. It is blocked by ATP in the cell, normally closed, and opened under various pathological or stress conditions. ATP production is reduced in sepsis, myocardial ischemia and hypoxia as well as other stress conditions, which results in the channel opening followed by cell membrane hyperpolarization, closing of voltage-dependent Ca2+ channels and reduced internal calcium current. Simultaneously, the opening of sarcKATP channels causes the outflow of K+ and accelerated repolarization, which results in shortening the duration of the cellular action potential and reduced inflow of Ca2+. In addition, due to the increase in ion outflow and rapid recovery of resting membrane potential, Ca2+ is easily released into the cytoplasm by Na+/Ca2+ exchange under a lower resting membrane potential. These mechanisms alleviate the extent of cellular damage from cytosolic Ca2+ overload. On the other hand, intracellular myocardial [Ca2+]i levels are reduced, leading to depression of the myocardical contraction, thereby reducing the cellular ATP consumption (5). Thus, sarcKATP channels mediate endogenous protective effects in myocytes by sensing and regulating the cellular levels of energy metabolism through electrical feedback, which helps the myocytes survive metabolic crisis. Genetic deletion or alterations to the Kir6.2 subunit of the sarcKATP channel results in a loss of both ischemic preconditioning and adaptation to stress (16–19) In addition, a reduction in the number of functional sarcKATP channels eliminates the transient action potentials during metabolic inhibition, leading to reduced metabolic stress tolerance, and increased Ca2+ loading during metabolic inhibition (20).
The role of sarcKATP channels in cardiac sepsis remains poorly understood. Many scholars have confirmed that the channel was activated or upregulated in response to LPS challenge. Since the sarcKATP channel plays an important role in maintaining cellular homeostasis, it mediates several cellular pathological and physiological activities, such as apoptosis. In the present study, P-1075, a selective sarcKATP opener, reduced LPS-induced apoptosis. By contrast, HMR-1098, a selective sarcKATP blocker, or the overexpression of Kir6.2AAA increased LPS-induced apoptosis in the cultured NRCs, as measured by flow cytometry and the TUNEL assay, which was consistent with the results of a previous study (10). LPS induced the apoptosis of cardiomyocytes. The sarcKATP channel prevented the apoptosis of cardiomyocytes induced by oxidative stress (21) and the apoptosis of the bladder smooth muscle induced by acute urinary retention (22). The channel also exerts a protective effect against ischemia-reperfusion-induced apoptosis in testicular damage (23).
As the cellular power houses, mitochondria are not only associated closely with the sarcKATP channel, but play a central role in apoptosis. Since mitochondria generate the majority of cellular ATP and its metabolite ADP in the cell, which are the main regulating factors of sarcKATP channels, mitochondria invariably affect the activity of sarcKATP channels. On the other hand, mitochondria also regulate ionic steady state in the cell. Under different stress conditions, mitochondria act as a buffer and reduce intracellular Ca2+ overload. However, uncontrolled Ca2+ overload initiates cell death including apoptosis (24). Mitochondrial Ca2+ overload triggers the opening of the mitochondrial permeability transport channel (mPTP), and the mitochondrial permeability transition (MPT), followed by phagocytosis by lysosomes. When a large number of mitochondria reach MPT, cytochrome c and apoptosis-inducing factor enter the cytoplasm, to activate cellular apoptosis, and even necrosis (25). In sepsis, mitochondrial function is impaired in cardiomyocytes and ATP production is reduced, which decreases sarcoplasm/endoplasmic reticulum (S/ER) calcium ATPase (SERCA) activity, leading to reduced levels of Ca2+ in the S/ER, increased levels of cytoplasmic Ca2+, resulting in mitochondrial Ca2+ accumulation (26). Opening sarcKATP channels reduces intracellular Ca2+overload by cell membrane hyperpolarization, shortening the action potential duration. Stimulating Na+-Ca2+ exchange also affects the mitochondrial Ca2+ load. In the present study, the mitochondrial calcium transport inhibitor RR partially attenuated the pro-apoptotic effect of HMR-1098. The results suggested that the mitochondrial pathway is involved in the sarcKATP-channel-mediated apoptosis of NRCs induced by LPS.
The present study provides new evidence correlating sarcKATP channel activity with downstream mitochondrial function. By elucidating the mechanism responsible for the sarcKATP channel-mediated regulation of apoptosis, we have provided a new perspective for the possible pathogenesis of sepsis being associated with cardiac dysfunction, which should facilitate the development of novel targeted therapies and strategies.
Acknowledgments
This study was supported by the grants from the National Natural Science Foundation of China (no. 81101450)
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