Upregulation of eukaryotic translation initiation factor 4E associates with a poor prognosis in gallbladder cancer and promotes cell proliferation in vitro and in vivo

Fang,Debao; Peng,Jing; Wang,Guobing; Zhou,Dachen; Geng,Xiaoping

doi:10.3892/ijmm.2019.4317

Upregulation of eukaryotic translation initiation factor 4E associates with a poor prognosis in gallbladder cancer and promotes cell proliferation in vitro and in vivo

Authors:
- Debao Fang
- Jing Peng
- Guobing Wang
- Dachen Zhou
- Xiaoping Geng
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Affiliations: Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230031, P.R China, Department of Surgery, The High‑Tech Zone Branch of The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230088, P.R China, Department of General Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230012, P.R China
Published online on: August 19, 2019 https://doi.org/10.3892/ijmm.2019.4317
Pages: 1325-1332
Copyright: © Fang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Eukaryotic translation initiation factor 4 (eIF4E) has been demonstrated to promote tumorigenesis in different types of cancer; however, whether eIF4E is involved in the development of GBC is unclear. The present study aimed to explore the biological function of eIF4E in gallbladder cancer (GBC) and identified that the expression level of eIF4E was significantly increased in GBC tissues compared with that in normal gallbladder tissues. The overall survival (OS) was also shorter in the group of patients with GBC with increased eIF4E expression. Increased eIF4E was correlated with advanced stage and higher histologic grade. Knockdown of eIF4E significantly inhibited cell proliferation, colony formation and cell cycle‑associated protein expression levels in 2 GBC cell lines. The weight of the tumors in the eIF4E knockdown group was remarkably decreased compared with the control group. It also was revealed that knockdown of eIF4E is associated with upregulating cyclin‑dependent kinase inhibitor 1B and downregulating the expression levels of cyclin E1 and cyclin D1 in vitro and in vivo. These data demonstrated that eIF4E is a novel prognostic marker in GBC and may serve a critical role in the regulation of cell proliferation.

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