Study on the <em>in vitro</em> changes of human bone marrow‑related mesenchymal stem cells

Wang,Cheng; Wang,Lu; Wang,Ziling; Yang,Zesong; Du,Kunhang; Song,Jiaqi; Hou,Jiying; Wang,Yaping

doi:10.3892/ijmm.2024.5464

Study on the in vitro changes of human bone marrow‑related mesenchymal stem cells

Authors:
- Cheng Wang
- Lu Wang
- Ziling Wang
- Zesong Yang
- Kunhang Du
- Jiaqi Song
- Jiying Hou
- Yaping Wang
View Affiliations

Affiliations: Department of Histology and Embryology, Laboratory of Stem Cells and Tissue Engineering, Chongqing Medical University, Chongqing 400016, P.R. China, Department of Hematology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Institute of Neuroscience, Chongqing Medical University, Chongqing 400016, P.R. China, Faculty of Basic Medical Sciences, Chongqing Medical and Pharmaceutical College, Chongqing 400016, P.R. China
Published online on: November 26, 2024 https://doi.org/10.3892/ijmm.2024.5464
Article Number: 23
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Bone marrow mesenchymal stem cells (MSCs) serve a pivotal role in the hematopoietic niche. The present study collected bone marrow samples from individuals across various age groups to investigate the biological characteristics of MSCs. By modifying the bone marrow microenvironment through co‑culture techniques, changes in the stemness of MSCs were examined. An in vitro hematopoietic co‑culture system was established to simulate the impact of MSCs on hematopoietic stem cells. The results demonstrated that the mode of cell‑to‑cell contact among stem cells is more influential in shaping bone marrow function compared with the effects of aging on these stem cells. Transcriptomic analysis revealed that MSCs serve as essential mediators, with their growth variations being both a consequence and a cause of changes in the bone marrow microenvironment. Furthermore, the decline in hematopoietic function observed in the elderly is a manifestation of this phenomenon. Data from the present study suggest that targeting MSCs is essential for enhancing bone marrow function and improving the outcomes of bone marrow transplantation.