search button

C-reactive protein is a biomarker of AFP-negative HBV-related hepatocellular carcinoma

  • Authors:
    • Sha She
    • Yi Xiang
    • Min Yang
    • Xiangchun Ding
    • Xiaoyan Liu
    • Lina Ma
    • Qing Liu
    • Bin Liu
    • Zhenhui Lu
    • Shiying Li
    • Yi Liu
    • Xiaoping Ran
    • Xiaoming Xu
    • Huaidong Hu
    • Peng Hu
    • Dazhi Zhang
    • Hong Ren
    • Yixuan Yang

  • View Affiliations

  • Published online on: June 10, 2015     https://doi.org/10.3892/ijo.2015.3042
  • Pages: 543-554
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Hepatocellular carcinoma (HCC) is one of the most aggressive cancers worldwide and is associated with the high rates of morbidity and mortality. α-fetoprotein (AFP) is common used in diagnosis of HCC; however, a growing body of research is questioning the diagnostic power of AFP. There is, therefore, an urgent need to develop additional novel non-invasive techniques for the early diagnosis of HCC, particularly for patients with AFP-negative [AFP(-)] HCC. Accordingly, in the present study, we employed iTRAQ-based mass spectro-metry to analyze the plasma proteins of subjects with AFP(-) HBV-related HCC, AFP(+) HBV-related HCC and non-malignant cirrhosis. We identified 14 aberrantly expressed proteins specific to the HCC patients, including 10 upregulated and 4 downregulated proteins. We verified C-reactive protein (CRP) overexpression by ELISA and immunohistochemical staining of clinical samples. Per ROC curve analyses, CRP was positive in 73.3% of patients with HBV-related HCC, and CRP overexpression had significant diagnostic power for AFP(-) HBV-related HCC. Furthermore, we found that silencing CRP caused a >2-fold decease in HBV replication. Additionally, we determined that this reduction in HBV replication involved the interferon-signaling pathway. However, silencing CRP also promoted HCC invasion and migration in vitro. In conclusion, we demonstrated that CRP can serve as a diagnostic biomarker for AFP(-) HBV-related HCC.
View Figures
View References

Related Articles

Journal Cover

August-2015
Volume 47 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 4.5
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 9.6
  • CiteScore Rank: Q1: #66/404 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.88
  • SCImago Journal Rank (SJR): 1.099
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
She S, Xiang Y, Yang M, Ding X, Liu X, Ma L, Liu Q, Liu B, Lu Z, Li S, Li S, et al: C-reactive protein is a biomarker of AFP-negative HBV-related hepatocellular carcinoma. Int J Oncol 47: 543-554, 2015.
APA
She, S., Xiang, Y., Yang, M., Ding, X., Liu, X., Ma, L. ... Yang, Y. (2015). C-reactive protein is a biomarker of AFP-negative HBV-related hepatocellular carcinoma. International Journal of Oncology, 47, 543-554. https://doi.org/10.3892/ijo.2015.3042
MLA
She, S., Xiang, Y., Yang, M., Ding, X., Liu, X., Ma, L., Liu, Q., Liu, B., Lu, Z., Li, S., Liu, Y., Ran, X., Xu, X., Hu, H., Hu, P., Zhang, D., Ren, H., Yang, Y."C-reactive protein is a biomarker of AFP-negative HBV-related hepatocellular carcinoma". International Journal of Oncology 47.2 (2015): 543-554.
Chicago
She, S., Xiang, Y., Yang, M., Ding, X., Liu, X., Ma, L., Liu, Q., Liu, B., Lu, Z., Li, S., Liu, Y., Ran, X., Xu, X., Hu, H., Hu, P., Zhang, D., Ren, H., Yang, Y."C-reactive protein is a biomarker of AFP-negative HBV-related hepatocellular carcinoma". International Journal of Oncology 47, no. 2 (2015): 543-554. https://doi.org/10.3892/ijo.2015.3042