Silencing of CDC20 suppresses metastatic castration-resistant prostate cancer growth and enhances chemosensitivity to docetaxel

  • Authors:
    • Ke Li
    • Yunhua Mao
    • Li Lu
    • Cheng Hu
    • Dejuan Wang
    • Jie Si-Tu
    • Minhua Lu
    • Shubin Peng
    • Jianguang Qiu
    • Xin Gao
  • View Affiliations

  • Published online on: August 23, 2016     https://doi.org/10.3892/ijo.2016.3671
  • Pages: 1679-1685
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Abstract

The role of cell division cycle 20 (CDC20) was investigated in chemoresistance to decetaxel and the underlying mechanisms in metastatic castration-resistant prostate cancer (mCRPC). MTT assays were performed to determine effects of siRNA-mediated CDC20 knockdown on cell proliferation and anticancer activity of docetaxel. Western blot analyses were conducted to detect changes of Akt and Wnt signaling. Furthermore, in vivo growth of PCa was examined in nude mice treated with siCDC20 or docetaxel alone or in combination. CDC20 was overexpressed in mCRPC cells. Knockdown of CDC20 suppressed cell proliferation and enhanced anticancer effect of docetaxel with IC50 reducing from 0.358 to 0.188 µg/ml in PC3 cells and 0.307 to 0.162 µg/ml in DU145 cells (P<0.01). While no change of Akt signaling was observed, inhibition of Wnt/β-catenin signaling was detected upon CDC20 silencing. Xenograft tumor growth was significantly reduced in nude mice by CDC20 inhibition. The additional treatment of siCDC20 achieved better anticancer effects than that of docetaxel alone. Silencing of CDC20 may be a new strategy to improve chemosensitization to docetaxel in mCRPC.
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October-2016
Volume 49 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Li K, Mao Y, Lu L, Hu C, Wang D, Si-Tu J, Lu M, Peng S, Qiu J, Gao X, Gao X, et al: Silencing of CDC20 suppresses metastatic castration-resistant prostate cancer growth and enhances chemosensitivity to docetaxel. Int J Oncol 49: 1679-1685, 2016.
APA
Li, K., Mao, Y., Lu, L., Hu, C., Wang, D., Si-Tu, J. ... Gao, X. (2016). Silencing of CDC20 suppresses metastatic castration-resistant prostate cancer growth and enhances chemosensitivity to docetaxel. International Journal of Oncology, 49, 1679-1685. https://doi.org/10.3892/ijo.2016.3671
MLA
Li, K., Mao, Y., Lu, L., Hu, C., Wang, D., Si-Tu, J., Lu, M., Peng, S., Qiu, J., Gao, X."Silencing of CDC20 suppresses metastatic castration-resistant prostate cancer growth and enhances chemosensitivity to docetaxel". International Journal of Oncology 49.4 (2016): 1679-1685.
Chicago
Li, K., Mao, Y., Lu, L., Hu, C., Wang, D., Si-Tu, J., Lu, M., Peng, S., Qiu, J., Gao, X."Silencing of CDC20 suppresses metastatic castration-resistant prostate cancer growth and enhances chemosensitivity to docetaxel". International Journal of Oncology 49, no. 4 (2016): 1679-1685. https://doi.org/10.3892/ijo.2016.3671