Association of Wilms' tumor 1 gene single-nucleotide polymorphism rs16754 with colorectal cancer

Sangkhathat,Surasak; Maneechay,Wanwisa; Chaiyapan,Welawee; Kanngern,Samornmas; Boonpipattanapong,Teeranut

doi:10.3892/mco.2015.647

Association of Wilms' tumor 1 gene single-nucleotide polymorphism rs16754 with colorectal cancer

Authors:
- Surasak Sangkhathat
- Wanwisa Maneechay
- Welawee Chaiyapan
- Samornmas Kanngern
- Teeranut Boonpipattanapong
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Affiliations: Department of Surgery, Tumor Biology Research Unit, Faculty of Medicine, Prince of Songkhla University, Songkhla 90110, Thailand, Central Research Laboratory, Faculty of Medicine, Prince of Songkhla University, Songkhla 90110, Thailand, Department of Biomedical Science, Biomedical Science Research Program, Faculty of Medicine, Prince of Songkhla University, Songkhla 90110, Thailand, Department of Pathology, Bumrungrad International Hospital, Bangkok 10110, Thailand
Published online on: September 16, 2015 https://doi.org/10.3892/mco.2015.647
Pages: 1401-1405

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Abstract

Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality worldwide. Our recent study demonstrated that the expression of Wilms' tumor 1 gene (WT1) is associated with surgical outcome in CRC patients. The present study aimed to investigate the genetic association of the single‑nucleotide polymorphism rs16754 in the WT1 gene with the occurrence of CRC, using an age‑matched case‑control study design. In addition, the correlation between genotype and WT1 expression was investigated. Genomic DNA samples from 104 CRC cases, aged 15‑65 years, and 208 healthy controls, were genotyped for rs16754 using the TaqMan genotyping method. The genotype distribution conformed to the Hardy‑Weinberg equilibrium (P=0.80). The overall minor allele frequency (MAF) of rs16754 (allele A) was 0.33. The MAF among CRC cases was significantly higher compared with that in controls (0.39 vs. 0.31, respectively; P=0.03). The AA genotype was significantly associated with the disease (odds ratio = 2.51, 95% confidence interval: 1.24‑5.07, P=0.01). Cases with the AA genotype exhibited a significantly poorer 3‑year overall survival (60%), compared with those with the GG or GA genotypes (80%) (log‑rank test, P<0.01). Reverse transcription quantitative polymerase chain reaction analysis demonstrated that the expression of WT1 in tumor tissues was higher compared with that in normal tissue; however, there were no significant differences in its expression among different genotypes. Therefore, rs16754 was found to be associated with the occurrence and prognosis of CRC in our subjects.

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