Lgr5-positive cells in the lung and their clinical significance in patients with lung adenocarcinoma

Zhang,Xiaohan; Xu,Miao; Su,Shixin; Zhou,Zeqi; Yang,Hong; Zhao,Suwen; Zeng,Danni; Yang,Kaiying; Liu,Yanan; Wang,Lijing; Li,Jiangchao

doi:10.3892/mco.2016.934

Lgr5-positive cells in the lung and their clinical significance in patients with lung adenocarcinoma

Authors:
- Xiaohan Zhang
- Miao Xu
- Shixin Su
- Zeqi Zhou
- Hong Yang
- Suwen Zhao
- Danni Zeng
- Kaiying Yang
- Yanan Liu
- Lijing Wang
- Jiangchao Li
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Affiliations: Vascular Biology Research Institute, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, State Key Laboratory of Oncology in South China, Sun Yat‑Sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China, Department of Thoracic Surgery, Sun Yat‑Sen University Cancer Center, Guangzhou, Guangdong 510060, P.R. China
Published online on: June 14, 2016 https://doi.org/10.3892/mco.2016.934
Pages: 283-288
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) is well-characterized as a marker of intestinal stem cells and certain types of tumor stem cells, where positive cells may develop into intestinal epithelial cells or intestinal adenomas. However, the roles of Lgr5 in the lung and in lung tumors remain unclear. An immunohistochemistry (IHC) analysis was performed to detect the expression of Lgr5 in the lung from a normal mouse. Histopathological sections of the lungs from Lgr5 heterozygous knockout mice (Lgr5+/‑) were observed following with hematoxylin and eosin. Furthermore, tissue microarrays containing tumor cores from lung cancer patients were also analyzed by IHC. Lgr5‑positive cells were present in the pulmonary alveoli and bronchi of normal mice, whereas the lungs of Lgr5+/‑ mice lost their normal morphological structure compared with the lungs of the normal mice. Lgr5 was expressed in lung adenocarcinoma, however, not in squamous carcinoma, and Lgr5 expression was positively associated with tumor, node, metastasis stage. Lgr5 is expressed in normal murine lung and is associated with TNM stage in patients with lung adenocarcinoma.

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