Correlation between SATB1 and Bcl-2 expression in human glioblastoma multiforme

Chu,Sheng-Hua; Ma,Yan-Bin; Feng,Dong-Fu; Li,Zhi-Qiang; Jiang,Pu-Cha

doi:10.3892/mmr.2012.1131

Correlation between SATB1 and Bcl-2 expression in human glioblastoma multiforme

Authors:
- Sheng-Hua Chu
- Yan-Bin Ma
- Dong-Fu Feng
- Zhi-Qiang Li
- Pu-Cha Jiang
View Affiliations

Affiliations: Department of Neurosurgery, No. 3 People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201900, P.R. China, Department of Neurosurgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, P.R. China
Published online on: October 16, 2012 https://doi.org/10.3892/mmr.2012.1131
Pages: 139-143

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Special AT-rich sequence-binding protein-1 (SATB1) has been reported to be overexpressed in numerous human tumors. The aim of the present study was to determine the correlation and clinical significance between the expression of SATB1 and B-cell lymphoma 2 (Bcl-2) in human glioblastoma multiforme (GBM). Samples from 70 patients with GBMs were analyzed and 10 normal brain tissues were used as the control group. In situ hybridization was used to detect SATB1 mRNA expression and immunohistochemistry was used to detect Bcl-2 and proliferating cell nuclear antigen (PCNA) protein expression. Apoptosis was detected with flow cytometry. The SATB1 mRNA and Bcl-2 protein levels were found to be significantly higher in GBM tissues than in normal brain and their levels were associated with patient survival, but not associated with patient gender, age and tumor size and site. A positive correlation was observed between SATB1 mRNA and Bcl-2 protein and between SATB1 mRNA and PCNA. A negative correlation was observed between SATB1 mRNA and apoptosis and between Bcl-2 and apoptosis. A positive correlation existed between Bcl-2 and PCNA. Patients with GBM identified as SATB1 mRNA (+) and Bcl-2 (+) were associated with a poor prognosis. Therefore, assessment of SATB1 and Bcl-2 co-expression may provide important information for the diagnosis, therapy and prognosis of GBM.

View Figures

View References

1	Wen PY and Kesari S: Malignant gliomas in adults. N Engl J Med. 359:492–507. 2008. View Article : Google Scholar : PubMed/NCBI
2	Chu SH, Ma YB, Feng DF, Zhang H, Zhu ZA, Li ZQ and Jiang PC: Correlation of low SLC22A18 expression with poor prognosis in patients with glioma. J Clin Neurosci. 19:95–98. 2012. View Article : Google Scholar : PubMed/NCBI
3	Chu SH, Feng DF, Ma YB, Zhang H, Zhu ZA, Li ZQ and Jiang PC: Promoter methylation and downregulation of SLC22A18 are associated with the development and progression of human glioma. J Transl Med. 9:1562011. View Article : Google Scholar : PubMed/NCBI
4	Chu SH, Ma YB, Feng DF, Zhang H, Qiu JH and Zhu ZA: Effect of 5-Aza-2′-deoxycytidine on SLC22A18 in glioma U251 cells. Mol Med Rep. 5:138–141. 2012.
5	Yang FY, Teng MC, Lu M, Liang HF, Lee YR, Yen CC, Liang ML and Wong TT: Treating glioblastoma multiforme with selective high-dose liposomal doxorubicin chemotherapy induced by repeated focused ultrasound. Int J Nanomedicine. 7:965–974. 2012. View Article : Google Scholar : PubMed/NCBI
6	Kouzarides T: Histone acetylases and deacetylases in cell proliferation. Curr Opin Genet Dev. 9:40–48. 1999. View Article : Google Scholar
7	Dickinson LA, Joh T, Kohwi Y and Kohwi-Shigematsu T: A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition. Cell. 70:631–645. 1992. View Article : Google Scholar : PubMed/NCBI
8	Han HJ, Russo J, Kohwi Y and Kohwi-Shigematsu T: SATB1 reprogrammes gene expression to promote breast tumour growth and metastasis. Nature. 452:187–193. 2008. View Article : Google Scholar : PubMed/NCBI
9	Meng WJ, Yan H, Zhou B, Zhang W, Kong XH, Wang R, Zhan L, Li Y, Zhou ZG and Sun XF: Correlation of SATB1 overexpression with the progression of human rectal cancer. Int J Colorectal Dis. 27:143–150. 2012. View Article : Google Scholar : PubMed/NCBI
10	Chen H, Takahara M, Oba J, Xie L, Chiba T, Takeuchi S, Tu Y, Nakahara T, Uchi H, Moroi Y and Furue M: Clinicopathologic and prognostic significance of SATB1 in cutaneous malignant melanoma. J Dermatol Sci. 64:39–44. 2011. View Article : Google Scholar : PubMed/NCBI
11	Liu M, Xiao GG, Rong P, Zhang Z, Dong J, Zhao H, Li H, Li Y, Pan J, Liu H, Wang W, Zha Q and Ju D: Therapeutic effects of Radix Dipsaci, Pyrola Herb and Cynomorium Songaricum on bone metabolism of ovariectomized rats. BMC Complement Altern Med. 12:672012.
12	Chu SH, Yuan XH, Jiang PC, Li ZQ, Zhang J, Wen ZH, Zhao SY, Chen XJ and Cao CJ: The expression of hepatocyte growth factor and its receptor in brain astrocytomas. Zhonghua Yi Xue Za Zhi. 85:835–838. 2005.(In Chinese).
13	Chu SH, Feng DF, Ma YB, Zhang H, Zhu ZA, Li ZQ and Zhang ZH: Expression of HGF and VEGF in the cerebral tissue of adult rats with chronic hydrocephalus after subarachnoid hemorrhage. Mol Med Rep. 4:785–791. 2011.PubMed/NCBI
14	Chu SH, Ma YB, Feng DF, Zhang H, Qiu JH and Zhu ZA: Elevated expression of solute carrier family 22 member 18 increases the sensitivity of U251 glioma cells to BCNU. Oncol Lett. 2:1139–1142. 2011.PubMed/NCBI
15	Zhang H, Liu L, Huang G, Zhou L, Wu W, Zhang T and Huang H: Protective effect of electroacupuncture at the Neiguan point in a rabbit model of myocardial ischemia-reperfusion injury. Can J Cardiol. 25:359–363. 2009. View Article : Google Scholar : PubMed/NCBI
16	Demurtas A, Aliberti S, Bonello L, Di Celle PF, Cavaliere C, Barreca A, Novero D and Stacchini A: Usefulness of multiparametric flow cytometry in detecting composite lymphoma: study of 17 cases in a 12-year period. Am J Clin Pathol. 135:541–555. 2011.PubMed/NCBI
17	Wang E, Hutchinson CB, Huang Q, Lu CM, Crow J, Wang FF, Sebastian S, Rehder C, Lagoo A, Horwitz M, Rizzieri D, Yu J, Goodman B, Datto M and Buckley P: Donor cell-derived leukemias/myelodysplastic neoplasms in allogeneic hematopoietic stem cell transplant recipients: a clinicopathologic study of 10 cases and a comprehensive review of the literature. Am J Clin Pathol. 135:525–540. 2011. View Article : Google Scholar
18	Chu SH, Feng DF, Zhang H, Chen ET, Duan ZX, Li XY, Li J, Ma YB, Zhu ZA and Qiu JH: c-Met-targeted RNA interference inhibits growth and metastasis of glioma U251 cells in vitro. J Neurooncol. 93:183–189. 2009. View Article : Google Scholar : PubMed/NCBI
19	Chu S, Yuan X, Li Z, Jiang P and Zhang J: C-Met antisense oligodeoxynucleotide inhibits growth of glioma cells. Surg Neurol. 65:533–538. 2006. View Article : Google Scholar : PubMed/NCBI
20	Lu X, Cheng C, Zhu S, Yang Y, Zheng L, Wang G, Shu X, Wu K, Liu K and Tong Q: SATB1 is an independent prognostic marker for gastric cancer in a Chinese population. Oncol Rep. 24:981–987. 2010.PubMed/NCBI
21	Wensveen FM, Alves NL, Derks IA, Reedquist KA and Eldering E: Apoptosis induced by overall metabolic stress converges on the Bcl-2 family proteins Noxa and Mcl-1. Apoptosis. 16:708–721. 2011. View Article : Google Scholar : PubMed/NCBI
22	Zhang MS, Hu AH, Qiu H, Xiong HH and Chen Y: The correlation between IGF-II and Bcl-2 expression in colorectal adenocarcinoma. Med Oncol. 29:928–932. 2012. View Article : Google Scholar : PubMed/NCBI
23	An YL, Nie F, Wang ZY and Zhang DS: Preparation and characterization of realgar nanoparticles and their inhibitory effect on rat glioma cells. Int J Nanomedicine. 6:3187–3194. 2011. View Article : Google Scholar : PubMed/NCBI