The tetraspanin CD151-ARSA mutant inhibits angiogenesis via the YRSL sequence Corrigendum in /10.3892/mmr.2023.13069

Peng,Dan; Zuo,Houjuan; Liu,Zhengxiang; Qin,Jin; Zhou,Yuanlin; Li,Pengcheng; Wang,Daowen; Zeng,Hesong; Zhang,Xin  A.

doi:10.3892/mmr.2012.1250

The tetraspanin CD151-ARSA mutant inhibits angiogenesis via the YRSL sequence

Corrigendum in: /10.3892/mmr.2023.13069

Authors:
- Dan Peng
- Houjuan Zuo
- Zhengxiang Liu
- Jin Qin
- Yuanlin Zhou
- Pengcheng Li
- Daowen Wang
- Hesong Zeng
- Xin A. Zhang
View Affiliations

Affiliations: Department of Nuclear Medicine of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R.China, Department of Cardiology of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R.China, Department of Physiology and Cancer Center, University of Oklahoma Health Science Center, Oklahoma City, OK, USA
Published online on: December 24, 2012 https://doi.org/10.3892/mmr.2012.1250
Pages: 836-842

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Previous studies have shown that the tetraspanin CD151 is essential for pathological or physiological angiogenesis. However, the cellular signaling mechanism and the role of the CD151 YRSL sorting motif in in vitro vasculogenesis remains unknown. In this study, the results showed that both CD151 and CD151-ARSA gene delivery were capable of increasing the expression of CD151 at the protein level in human umbilical vein endothelial cells (HUVECs). Moreover, there was no significant difference in CD151 protein expression between the CD151 group and the CD151-ARSA group. Overexpression of CD151 promoted HUVEC cell proliferation, migration and capillary network formation in vitro. However, in the CD151-ARSA group, the abilities of cell proliferation, migration and capillary network formation were all decreased, compared with the CD151 group. Furthermore, the activation of PI3K, Akt and ERK signaling pathways was attenuated in the CD151-ARSA mutant group compared with the CD151 group. This study suggests that the YRSL motif of CD151 plays a key role in CD151-induced angiogenesis. Our observations provide insights into a new mechanism of CD151 regulating angiogenesis via vesicle trafficking.

View Figures

View References

1	Fitter S, Tetaz TJ, Berndt MC and Ashman LK: Molecular cloning of cDNA encoding a novel platelet-endothelial cell tetra-span antigen, PETA-3. Blood. 86:1348–1355. 1995.PubMed/NCBI
2	Hasegawa H, Utsunomiya Y, Kishimoto K, Yanagisawa K and Fujita S: SFA-1, a novel cellular gene induced by human T-cell leukemia virus type 1, is a member of the transmembrane 4 superfamily. J Virol. 70:3258–3263. 1996.PubMed/NCBI
3	Sincock PM, Mayrhofer G and Ashman LK: Localization of the transmembrane 4 superfamily (TM4SF) member PETA-3 (CD151) in normal human tissues: comparison with CD9, CD63, and alpha5beta1 integrin. J Histochem Cytochem. 45:515–525. 1997. View Article : Google Scholar : PubMed/NCBI
4	Sincock PM, Fitter S, Parton RG, Berndt MC, Gamble JR and Ashman LK: PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function. J Cell Sci. 112:833–844. 1999.PubMed/NCBI
5	Yauch RL, Berditchevski F, Harler MB, Reichner J and Hemler ME: Highly stoichiometric, stable, and specific association of integrin alpha3beta1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration. Mol Biol Cell. 9:2751–2765. 1998. View Article : Google Scholar : PubMed/NCBI
6	Zhang XA, Kazarov AR, Yang X, Bontrager AL, Stipp CS and Hemler ME: Function of the tetraspanin CD151-alpha6beta1 integrin complex during cellular morphogenesis. Mol Biol Cell. 13:1–11. 2002. View Article : Google Scholar : PubMed/NCBI
7	Hemler ME: Integrin associated proteins. Curr Opin Cell Biol. 10:578–585. 1998. View Article : Google Scholar
8	Serru V, Le Naour F, Billard M, et al: Selective tetraspan-integrin complexes (CD81/alpha4beta1, CD151/alpha3beta1, CD151/alpha6beta1) under conditions disrupting tetraspan interactions. Biochem J. 340:103–111. 1999. View Article : Google Scholar : PubMed/NCBI
9	Zhang XA, Bontrager AL and Hemler ME: Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta(1) integrins. J Biol Chem. 276:25005–25013. 2001. View Article : Google Scholar : PubMed/NCBI
10	Wright MD, Geary SM, Fitter S, et al: Characterization of mice lacking the tetraspanin superfamily member CD151. Mol Cell Biol. 24:5978–5988. 2004. View Article : Google Scholar : PubMed/NCBI
11	Takeda Y, Kazarov AR, Butterfield CE, et al: Deletion of tetraspanin Cd151 results in decreased pathologic angiogenesis in vivo and in vitro. Blood. 109:1524–1532. 2007. View Article : Google Scholar : PubMed/NCBI
12	Lan RF, Liu ZX, Liu XC, Song YE and Wang DW: CD151 promotes neovascularization and improves blood perfusion in a rat hind-limb ischemia model. J Endovasc Ther. 12:469–478. 2005. View Article : Google Scholar : PubMed/NCBI
13	Zuo H, Liu Z, Liu X, et al: CD151 gene delivery after myocardial infarction promotes functional neovascularization and activates FAK signaling. Mol Med. 15:307–315. 2009.PubMed/NCBI
14	Liu WF, Zuo HJ, Chai BL, et al: Role of tetraspanin CD151-alpha3/alpha6 integrin complex: Implication in angiogenesis CD151-integrin complex in angiogenesis. Int J Biochem Cell Biol. 43:642–650. 2011. View Article : Google Scholar : PubMed/NCBI
15	Zheng ZZ and Liu ZX: Activation of the phosphatidylinositol 3-kinase/protein kinase Akt pathway mediates CD151-induced endothelial cell proliferation and cell migration. Int J Biochem Cell Biol. 39:340–348. 2007. View Article : Google Scholar : PubMed/NCBI
16	Zuo H, Liu Z, Yang J, et al: Activation of the ERK signaling pathway is involved in CD151-induced angiogenic effects on the formation of CD151-integrin complexes. Acta Pharmacol Sin. 31:805–812. 2010. View Article : Google Scholar : PubMed/NCBI
17	Zuo H, Liu Z, Liu X, et al: Assessment of myocardial blood perfusion improved by CD151 in pig myocardial infarction model. Acta Pharmacol Sin. 30:70–77. 2009. View Article : Google Scholar : PubMed/NCBI
18	Bretscher MS and Aguado-Velasco C: Membrane traffic during cell locomotion. Curr Opin Cell Biol. 10:537–541. 1998. View Article : Google Scholar : PubMed/NCBI
19	Kobayashi T, Vischer UM, Rosnoblet C, et al: The tetraspanin CD63/lamp3 cycles between endocytic and secretory compartments in human endothelial cells. Mol Biol Cell. 11:1829–1843. 2000. View Article : Google Scholar : PubMed/NCBI
20	Duffield A, Kamsteeg EJ, Brown AN, Pagel P and Caplan MJ: The tetraspanin CD63 enhances the internalization of the H,K-ATPase beta-subunit. Proc Natl Acad Sci USA. 100:15560–15565. 2003. View Article : Google Scholar : PubMed/NCBI
21	Caswell PT, Vadrevu S and Norman JC: Integrins: masters and slaves of endocytic transport. Nat Rev Mol Cell Biol. 10:843–853. 2009. View Article : Google Scholar : PubMed/NCBI
22	Liu L, He B, Liu WM, Zhou D, Cox JV and Zhang XA: Tetraspanin CD151 promotes cell migration by regulating integrin trafficking. J Biol Chem. 282:31631–31642. 2007. View Article : Google Scholar : PubMed/NCBI
23	Ohno H, Stewart J, Fournier MC, et al: Interaction of tyrosine-based sorting signals with clathrin-associated proteins. Science. 269:1872–1875. 1995. View Article : Google Scholar : PubMed/NCBI
24	Bonifacino JS and Traub LM: Signals for sorting of transmembrane proteins to endosomes and lysosomes. Annu Rev Biochem. 72:395–447. 2003. View Article : Google Scholar : PubMed/NCBI
25	Auricchio A, Hildinger M, O’Connor E, Gao GP and Wilson JM: Isolation of highly infectious and pure adeno-associated virus type 2 vectors with a single-step gravity-flow column. Hum Gene Ther. 12:71–76. 2001. View Article : Google Scholar : PubMed/NCBI
26	Berditchevski F and Odintsova E: Characterization of integrin-tetraspanin adhesion complexes: role of tetraspanins in integrin signaling. J Cell Biol. 146:477–492. 1999. View Article : Google Scholar : PubMed/NCBI
27	Fitter S, Sincock PM, Jolliffe CN and Ashman LK: Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion. Biochem J. 338:61–70. 1999. View Article : Google Scholar : PubMed/NCBI
28	Conner SD and Schmid SL: Regulated portals of entry into the cell. Nature. 422:37–44. 2003. View Article : Google Scholar : PubMed/NCBI
29	Carmeliet P: Mechanisms of angiogenesis and arteriogenesis. Nat Med. 6:389–395. 2000. View Article : Google Scholar : PubMed/NCBI
30	Rey S and Semenza GL: Hypoxia-inducible factor-1-dependent mechanisms of vascularization and vascular remodelling. Cardiovasc Res. 86:236–242. 2010. View Article : Google Scholar : PubMed/NCBI
31	Ulrich F and Heisenberg CP: Trafficking and cell migration. Traffic. 10:811–818. 2009. View Article : Google Scholar : PubMed/NCBI
32	Mellman I and Nelson WJ: Coordinated protein sorting, targeting and distribution in polarized cells. Nat Rev Mol Cell Biol. 9:833–845. 2008. View Article : Google Scholar : PubMed/NCBI