Role of Nogo‑A in the regulation of hepatocellular carcinoma SMMC‑7721 cell apoptosis

  • Authors:
    • Chun‑Qiu Hao
    • Yun Zhou
    • Jiu‑Ping Wang
    • Mei‑Jun Peng
    • Yu‑Mei Xie
    • Wen‑Zhen Kang
    • Li Sun
    • Ping‑Zhong Wang
    • Chun‑Ling Wan
    • Lin He
    • Lei Cai
    • Zhang‑Sheng Jia
  • View Affiliations

  • Published online on: March 13, 2014     https://doi.org/10.3892/mmr.2014.2050
  • Pages: 1743-1748
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Abstract

Nogo‑A has been identified as an inhibitor of neurite outgrowth specific to the central nervous system. However, little is known about the role of Nogo‑A in hepatocellular carcinoma (HCC), the most common primary malignant tumor with a high mortality rate. This study aimed to investigate the role of endogenous Nogo‑A in human liver cancer cells. Reverse transcription polymerase chain reaction was used to detect the expression of Nogo‑A in four liver cancer cell lines. A lentivirus vector was then constructed to mediate RNA interference (RNAi) targeting of Nogo‑A (LV‑Nogo‑A‑siRNA) and was confirmed to successfully suppress the expression of the Nogo‑A gene in SMMC‑7721 cells. Furthermore, Nogo‑A was observed to be highly expressed in liver cancer cell lines. RNAi of Nogo‑A using the LV‑Nogo‑A‑siRNA construct significantly decreased Nogo‑A protein expression and specifically inhibited the growth of SMMC‑7721 cells. This growth inhibitory effect may be attributed to an increase in G2/M phase arrest and apoptosis in SMMC‑7721 cells containing Nogo‑A‑siRNA. The results of this study demonstrate that Nogo‑A may represent a novel therapeutic target for the treatment of liver cancer, in addition to its potent roles in neural systems.
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May-2014
Volume 9 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Hao CQ, Zhou Y, Wang JP, Peng MJ, Xie YM, Kang WZ, Sun L, Wang PZ, Wan CL, He L, He L, et al: Role of Nogo‑A in the regulation of hepatocellular carcinoma SMMC‑7721 cell apoptosis. Mol Med Rep 9: 1743-1748, 2014
APA
Hao, C., Zhou, Y., Wang, J., Peng, M., Xie, Y., Kang, W. ... Jia, Z. (2014). Role of Nogo‑A in the regulation of hepatocellular carcinoma SMMC‑7721 cell apoptosis. Molecular Medicine Reports, 9, 1743-1748. https://doi.org/10.3892/mmr.2014.2050
MLA
Hao, C., Zhou, Y., Wang, J., Peng, M., Xie, Y., Kang, W., Sun, L., Wang, P., Wan, C., He, L., Cai, L., Jia, Z."Role of Nogo‑A in the regulation of hepatocellular carcinoma SMMC‑7721 cell apoptosis". Molecular Medicine Reports 9.5 (2014): 1743-1748.
Chicago
Hao, C., Zhou, Y., Wang, J., Peng, M., Xie, Y., Kang, W., Sun, L., Wang, P., Wan, C., He, L., Cai, L., Jia, Z."Role of Nogo‑A in the regulation of hepatocellular carcinoma SMMC‑7721 cell apoptosis". Molecular Medicine Reports 9, no. 5 (2014): 1743-1748. https://doi.org/10.3892/mmr.2014.2050