Post‑conditioning with sevoflurane induces heme oxygenase‑1 expression via the PI3K/Akt pathway in lipopolysaccharide‑induced acute lung injury

  • Authors:
    • Shuangping Zhao
    • Jiao Wu
    • Lemeng Zhang
    • Yuhang Ai
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  • Published online on: March 31, 2014     https://doi.org/10.3892/mmr.2014.2094
  • Pages: 2435-2440
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Abstract

The aim of the present study was to explore the regulatory mechanism of heme oxygenase‑1 (HO‑1) expression induced by sevoflurane (Sevo) in lipopolysaccharide (LPS)‑induced acute lung injury (ALI). Sprague‑Dawley rats were divided randomly into six groups: (A) Control, (B) 2.4% Sevo only, (C) LY294002 (PI3K inhibitor) only, (D) LPS + 2.4% Sevo, (E) LY294002 + LPS + 2.4% Sevo and (F) LPS only. The pathological changes in wet/dry weight ratio (W/D), the activities of superoxide dismutase, myeloperoxidase (MPO), malondialdehyde, and HO‑1, as well as the expression of intercellular adhesion molecule (ICAM‑1), HO‑1, phospho‑phosphatidylinositol 3‑kinase (pPI3K) and phospho‑Akt (pAkt) were recorded. Sevo post‑conditioning was able to effectively protect from ALI with decreasing pathomorphological scores, MPO activity, W/D and the mRNA and protein expression levels of ICAM‑1. Sevo promotes HO‑1 expression via the PI3K/protein kinase B (PI3K/Akt) pathway with activation of pPI3K and pAkt. Inhibition of the PI3K/Akt pathway by LY294002 partly eliminates the protective effects of Sevo. It is concluded that Sevo post‑conditioning has a vital role in inducing the upregulation of HO-1 expression via the PI3K/Akt pathway to alleviate ALI.
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June-2014
Volume 9 Issue 6

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Zhao S, Wu J, Zhang L and Ai Y: Post‑conditioning with sevoflurane induces heme oxygenase‑1 expression via the PI3K/Akt pathway in lipopolysaccharide‑induced acute lung injury. Mol Med Rep 9: 2435-2440, 2014
APA
Zhao, S., Wu, J., Zhang, L., & Ai, Y. (2014). Post‑conditioning with sevoflurane induces heme oxygenase‑1 expression via the PI3K/Akt pathway in lipopolysaccharide‑induced acute lung injury. Molecular Medicine Reports, 9, 2435-2440. https://doi.org/10.3892/mmr.2014.2094
MLA
Zhao, S., Wu, J., Zhang, L., Ai, Y."Post‑conditioning with sevoflurane induces heme oxygenase‑1 expression via the PI3K/Akt pathway in lipopolysaccharide‑induced acute lung injury". Molecular Medicine Reports 9.6 (2014): 2435-2440.
Chicago
Zhao, S., Wu, J., Zhang, L., Ai, Y."Post‑conditioning with sevoflurane induces heme oxygenase‑1 expression via the PI3K/Akt pathway in lipopolysaccharide‑induced acute lung injury". Molecular Medicine Reports 9, no. 6 (2014): 2435-2440. https://doi.org/10.3892/mmr.2014.2094